Online ISSN: 2515-8260

Keywords : fungi


STUDY of MICROBIAL AIR CONTAMINATION IN BIOLOGICAL LABORATORIES/ COLLEGE of SCIENCE

Dunia K. Salim

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 123-129

Objective: A study on indoor air play important roles in human health by polluted indoor labrotory and causes some diseases such as infections skin, eyes, respiratory system , allergy and biotoxicity. Air is made up of enormous of microorganisms mainly bacteria. Their estimation is important as an indication of cleanliness of any particular environment. The presence both bacteria and fungi in indoor air is problem of health protection due to a long time period that workers and students stay indoors. Determination of level microbial pollution indoors is necessary to assess the health hazard for indoor air quality control. This study provides information on microbial contamination level of indoor air of biological labrotary was estimated. Material and Methods: Collected air samples from seven bio-laboratories and low, up corridors locations during April-June 2019. Air samples were taken three months : in the in the afternoon. Results: The higher of percentage of isolated airborne bacteria Staphylococcus aureus and Staphylococcus epidermis, it was 58.57% and 26.84% for all biological laboratories respectively at study location, while the lowest percentage were Klebsiella sp. and Protus sp., it was 11.98% and 4.29% for all biological laboratories, respectively. Conclusion: It is concluded from the current study that is advisable that estimate to check microorganisms loads in Laboratories and prevent their increase and spread

Antibacterial Finishing and Dyeing Affinity Enhancement of cellulose-based fabrics via pre-treatment by chitosan nanoparticles treatment

Fatma A. Mohamed; Maysa M. Reda

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 10, Pages 2378-2392

Chitosan nanoparticles were prepared as green antibacterial finishing material and enhance antimicrobial activity of cellulose-based fabrics such as cotton and viscose. Two new bifunctional dye Bis (monochlorotriazine) (MCT) and hetero bifunctional (SES/MCT) reactive dyes have been prepared in previous work. Dimedone moiety (5,5-Dimethylcyclohexane-1,3-dione) and applied in cellulose-based fabrics with various chitosan nanoparticles concentrations imparting them antimicrobial activity. In this work the effect of chitosan nanoparticles on dye ability of cellulose-based fabrics were studied from evaluation of the colour strength expressed in K/S values of the dyed fabrics at different concentration of chitosan nanoparticles and the two synthesized used dyes. Results showed that chitosan nanoparticles treated cellulose-based fabrics have higher affinity towards dyeing than untreated cellulose-based fabrics. Also, the antimicrobial properties of the treated fabrics were higher than that of untreated fabrics and give higher exhaustion without using any salts and alkalinity. So, chitosan nanoparticles exhibit cellulose based fabrics high antimicrobial and having dyeing affinity than untreated one. Also, treated dyed fabrics showed very good to excellent fastness properties.

Isolation and Identification of fungi from post-harvest stored onion varieties and their control measures with organic formulations

Vinay Kumar; Neeraj .

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 2664-2675

The research work entitled “Studies on preventive measures to reduce the postharvest losses in onion (Allium cepa L.)” was carried out at NIFTEM, Sonepat, Haryana, India during Rabi seasons of 2014-15 and 2015-16. The research work was carried out with objectives of studying the effect of application of organic formulations (applied as pre-harvest foliar spray viz., Neem based formulation (Besara) at the rate of 20ml/L; Panchgavya at the rate of 100ml/L; Trichoderma viride (Bio Shield) at the rate of 50g/L) on growth period of selected onion varieties (Pusa Madhavi, Pusa Riddhi, Pusa Red, NHRDF Red and Agri found Light Red) grown during Rabi season in Haryana region. The formulations were applied as four pre-harvest foliar sprays starting from 30 days after transplanting and repeated at an interval of 15 days. The main aim of this experiment was to isolate and identify the pathogenic fungi causing spoilage of stored onion bulbs under cold and ambient conditions. Additionally, the efficacy of organic formulations against the isolated pathogenic fungi was studied under both the onion storage conditions. Fungal isolates responsible for disease and rotting as identified under cold storage condition were Penicillum purpurogenum, Penicillum griseofalvum, Penicillum citrinum and as Aspergillus niger under ambient storge contion. Under in-vitro condition, Neem based formulation (Besaara) was most effective for inhibition of fungal growth. The efficacy of organic formulations in restricting the fungal growth was higher with higher concentration (i.e. at 20%).