European Journal of Molecular & Clinical Medicine

Background: Psoriasis is a chronic relapsing, immune-mediated inflammatoryskin disease of unknown etiology. The role of thrombomodulin (TM) in different autoimmune inflammatory diseases was explored previously in some studies. Thus; the aim of this study was to assess TM expression in the affected skin of patients with psoriasis.
Methods: This prospective case-control study was carried out on 30 patients with chronic plaque psoriasis and 30 age and gender matched apparently normal subjects as a control group. Skin biopsies were taken for immunohistochemical evaluation of TM expression.
Results: There was a highly significant difference between the two studied groups regarding the expression of TM in the skin. Patients group had a higher expression of TM in both epidermis and dermis compared to controls with a highly statistically significant difference, P-value< 0.01.

Background: Psoriasis is a chronic inflammatory skin disease has a strong genetic predisposition. Aim of the work: To study the role of TNFAIP3 rs610604 (C/A) polymorphism in psoriasis, and its effect on Etanercept response. Subjects and methods: One hundred patients with psoriasis, in addition 100 apparently healthy individuals as a control group were included in this study. Restriction Fragment Length Polymorphism-Polymerase Chain Reaction (RFLP-PCR) was performed to evaluate different TNFAIP3 gene polymorphism. TNFAIP3 serum levels were assessed by enzyme-linked immunosorbent assay. Results: Current study showed low serum level of TNFAIP3 protein in psoriatic patients when compared with control group (P<0.01). A considerably higher risk of psoriasis was observed in individuals who had TNFAIP3 rs610604 SNP CC and C allele than people carrying AA genotype and A allele. In relation to the response to treatment, this study observed that the mutant homozygous genotype CC was more frequent in non -responder (71.4%) than responder (1.3%) patients (P<0.01). Conclusion: TNFAIP3 rs610604 (C/A), might be useful for prognosis of psoriatic patients

The choice of therapy in mild to moderate level of psoriasis includes topical therapy. In silico is an analog computerized experiment which is similar to in vivo and in vitro biological experiment. Cyperus rotundus or nut grass has antipsoriatic activity so that it can be used as a topical herbal medicine in reducing psoriasis severity. The aim of this study was intended to evaluate the content of Cyperus rotundus using in silico analysis in psoriasis treatment. Active compound of extracted Cyperus rotundus is taken from the knapsack database. Simplified Molecular Input Line Entry System (SMILE) format is taken from the pubchem database. The predictions on skin barrier repair, immunosuppressors, anti-inflammatory and antipruritic agents are performed by using a pass server. The molecular mechanism of active compounds in the human body is taken from the search tool for interacting chemicals (STITCH) which is experimentally predicted; then it is computationally analyzed. Further pathway analysis uses cytoscape software. There are 21 active compounds on Cyperus rotundus. The potential of Cyperus rotundus as immunosuppressor, anti-inflammatory, and antipruritic is predicted to have activity that is examined computationally, but this has not been proven in laboratory experiment or has small potential. The highest potential of Cyperus rotundus bioactivity in improving skin barrier which has the most function is beta-selinene with an average value of probable to be active (Pa) 0.715 predicted to have high potential computationally and laboratory tests. Overall, Cyperus rotundus is a good option for psoriasis therapy in improving skin barrier function, immunosuppressors, anti-inflammatory and antipruritic because it has beneficial effectiveness in terms of the availability and safety.

Background/Rationale: Skin is the most visible organ that defines our appearance and plays a significant social communication role to a great extent. Human beings are often concerned with their external physical appearance, which is directly related to the health of the skin, and if any disease triggers a change in the skin's natural appearance, it causes distress to the individual. Chronic diseases such as Vitiligo, Psoriasis, and Pemphigus severely dagrades the quality of life ( QOL) of affected individuals. ,Psychiatric morbidity was shown by Indian patients as stress ,anxiety depression, lower self-esteem, adjustment disorders, and sleep disturbance, and are vulnerable to various psychiatric disorders such as severe depression and suicidal ideations. The involvement of a chronic illness is typically associated with lower health-related quality of life ( HRQOL), and severity of the disorder often affects HRQOL. Objectives: To evaluate the quality of life of patients having Psoriasis, Vitiligo and Pemphigus Vulgaris and To explore the correlation between disease severity and quality of life. Methods: Prospective Cross sectional Study will be carried out in 65 patients with chronic skin diseases, attending Out Patient Department of Dermatology, AVBRH, JNMC, Sawangi, Wardha, will be Registered after having considered different conditions for inclusion and exclusion. A detailed history will be taken, severity index will be calculated and questionnaire, DLQI, HRQOL will be given to the patients. Results: The result would be undertaken in SPSS software. Conclusion: The conclusion will be based on findings for study protocol.