Online ISSN: 2515-8260

Keywords : Hospital acquired infection


MOLECULAR DETECTION of PANTON VALENTINE LEUKOCIDIN (PVL) GENES in METHICILLIN-RESISTANCE STAPHYLOCOCCUS AUREUS (MRSA) ISOLATED FROM BURNS INFECTION

Thanaa R. Abdulrahman; Dhefaf R. Mahdi; Jabbar S. Hassan

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 254-259

Background: Methicillin resistance Staphylococcus aureus (MRSA) infection related to community has been enhanced through the world. One of the significant cytotoxins elaborated by a few strains of S. aureus is the Panton Valentine leukocidin (PVL), coded by two genes, lukS- PV and lukF-PV which is element of toxin that created pores in the membranes of cells, the role of PVL in the disease process severity of Staphylococcus aureus till known is debated. Amis: This study was planned to detect the Panton Valentine leucocidin (PVL)genes and MRSA isolated from Burn wound infection in community and hospital acquired settings. Material and methods: Cross-sectional study involved 200 patients who presented with burn wounds from third-degree or fourth-degree. The bacteria from all samples were undergone to Cefoxitin disc diffusion testing utilization a 30 μg cefoxitin disc for methicillin resistant determination. Following DNA extraction, conventional polymerase chain reaction (PCR) was used to detect PVL genes. Results: Twenty-eight samples were positive culture for Staph. aureus, of which 19 (67.8%) patients presented with CAI, and 9 (32.1%) belong Hospital acquired infection A total of 23 (82.1%) showed methicillin resistance. Out of 28 isolates, 7(25%) isolates were harboring PVL gene, all of which were Methicillin resistance community acquired infection. None of S. aureus isolates from hospital acquired infection had PVL. Conclusion: This study indicated high prevalence of PVL among community acquired infection MRSA isolates, and the lack of this gene in Hospital acquired infection

ROLE OF TASK FORCE COMMITTEE IN HOSPITAL INFECTION CONTROL DURING EPIDEMICS AND PANDEMICS

Nirubama.K .; A.S.Smiline Girija; Ezhilarasan D

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 2021-2029

Pandemics are defined as large- scale outbreaks that can greatly increase mortality and morbidity over a wide geographic area and cause significant economic, political and social disruption due to spread of infectious disease. Risk of pandemic and epidemic conditions have occurred throughout history and appear to be increasing in frequency, particularly because of the increasing emergence of viral disease from animals. Other associated risk factors include older age, longer hospital stays, immunosuppression, frequent encounters with healthcare facilities, mechanical ventilatory support, multiple underlying chronic illnesses, recent invasive procedures, indwelling devices and being in a critical care unit. Management of acute infections transmitted to susceptible patients via cross infections and emergence of antimicrobial- resistant pathogens need attention in a hospital set-up. Task forces committee are organisations of small groups of resources and people brought together to establish a specific objective, to complete the expectation that the group will disband when the objective has been completed. The task force committee is actively involved in resolving issues on hospital acquired infection during pandemic and epidemic outbreak of disease and this review underpins the same.