European Journal of Molecular & Clinical Medicine

Infertility in women is common over the world, and lipid abnormalities are thought to play a role. The goal of this study was to determine the plasma lipid profile and atherogenicity indices among infertile women who visited assisted reproductive technology clinics. In 140 infertile women and 50 healthy age-matched women of proven fertility, the serum lipid profile (total cholesterol, triglycerides, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, and computed indicators of atherogenicity) was assessed.Using reagents provided by Randox Laboratories, Crumlin, Antrim, UK, the lipid profile was determined using the spectrophotometric method. The unpaired Students' test was used to compare the mean values of measured parameters between cases and controls. Age (p<0.001), total cholesterol, triglycerides, low-density lipoprotein, AIP, certain cardiac risk ratios, and atherogenic coefficients were considerably greater (p<0.001) in infertile women than in control participants, but high-density cholesterol was significantly lower (p<0.001). The difference in mean BMI between the patients and controls was not statistically significant. Except for the high density/low-density ratio, all atherogenicity indices were considerably greater in infertile women seeking assisted reproductive technology for conception than in control participants. This group of people has greater atherogenicity indices, which may predispose them to cardiovascular disease. As a result, it is recommended that lipid profiles and atherogenicity indices be evaluated on a regular basis.

The role of an interaction between PLCγ and the proteins during fertilization is still unclear. To recognize whether fertilization includes PLCβ- Cа2+ and PLCγ release mechanisms, GST-SH2 fusion protein was designed and injected into the egg of Asterina miniata sp. In this study, the mixture of SH2-pGEMT-Easy vector was combined with the component cell of DH5α, the results proved the success of SH2 domains insertion into SH2-pGEMT-Easy vector using AmPLCγ plasmid. AmPLCγ was treated with EcoR1 to make a GST-SH2 fusion protein. The designed new SH2 primer including EcoR1 and Xho 1 digestion site and clone them into pGEX vector system, could be directly detect the interaction protein with SH2 domain with an easy purification