European Journal of Molecular & Clinical Medicine

Diabetesis characterized by chronic hyperglycemia & disturbances of carbohydrate, lipid and protein metabolism. In subtypes of Diabetes Mellitus, type II diabetes mellitus is the most common endocrine and metabolic disorder. DM is a condition of increased oxidative stress and requires antioxidant.
Aim: To assess the association between of oxidative stress and lipid abnormalities in type 2 diabetes mellitus patients in comparison with healthy subjects.
Materials & Method: This is a case control study, conducted in Department of Biochemistry,
Era’s Lucknow Medical University, Lucknow, U.P., India between January 2022 to June 2022.  
A total of 90 cases of type II diabetes mellitus & 90 cases of healthy subjects between  the age
of 30 and 60 years were enrolled in study. Fasting blood glucose, glycated hemoglobin  (HbA1c),
 total cholesterol (TC), triglycerides (TG), high density lipid (HDL) and  malondialdehyde (MDA)
 levels were assessed.
 
Results: The mean age of type II diabetes mellitus cases were higher 55.2±10.1yr and 45±14 yrs in healthy subjects. The body mass index was higher 29.7±3.8 kg/m² and 24.8±5.3 in healthy controls. Significant difference was observed in FBS, Hb1Ac, TC, TG, LDL, HDL along with VLDL & MDA. With TC, LDL, VLDL, FBS, HbA1c shows positive correlation between MDA and HDL.
Conclusion: Increased blood glucose levels along with dyslipidemia in patients with diabetes causes oxidative stress leading to atherosclerosis.Early detection and treatment of lipid abnormalities may be used to minimize risk for atherogenic cardiovascular disorder & cerebrovascular accident in patients with diabetes.

Recurrent pregnancy loss (RPL), known popularly as recurrent miscarriage, is a clinical condition of two or more failed pregnancies, affecting 12-15% of total pregnancies. Besides genetic and anatomical abnormalities, stress and anxiety impart a vital role in repeated abortions. The Perceived Stress Scale, an extensively used psychological tool for assessing the perception of stress in an individual, was taken for evaluating the role of psychological stress among subjects with RPL. The oxidative stress marker, malondialdehyde (MDA) and several other clinical parameters were also analyzed among 150 women with RPL and 110 healthy women with one or two children. The study concluded that RPL individuals are shown to have higher rates of oxidative and psychological stress with elevated MDA concentration and PSS score respectively. 

Background: Diabetes mellitus (DM) has become a major and serious threat to global human health. The present study assessed high-sensitivity C-reactive protein, malondialdehyde and their association with glycated hemoglobin in type II diabetes patients.
Materials & Methods: Group I comprised of 96 type II diabetics and group II had 96 healthy control subjects. HbA1c, serum insulin, hs- CRP, Homeostasis model assessment for insulin resistance, serum creatinine and malondialdehyde (MDA) levels were measured.
Results: Group I had 56 males and 40 females and group II had 48 males and 48 females. HOMA- IR was 4.8 in group I and 1.7 in group II, MDA level in group I was 5.07 µmol/L and in group II was 1.31 µmol/L. Creatinine level was 0.98 mg/L in group I and 0.85 mg/L in group II, cystatin C was 0.85 mg/L in group I and 0.92 mg/L in group II. The mean Hs-CRP (mg/L) level was 4.6 in group I and 1.9 in group II. The difference was significant (P< 0.05).
Conclusion: Type II diabetes mellitus patients had elevated level of malondialdehyde and high-sensitivity C-reactive protein as compared to healthy control subjects

Background:Diabetes mellitus is a syndrome characterized by chronic hyperglycemia and the most common complications such as atherosclerosis, nerve damage, renal failure, male impotence and infection. The present study was conducted to assess total antioxidant status in relation to oxidative stress in type II diabetes.
Materials & Methods: 90 type II diabetes patients and healthy subjects of both genders were subjected to assessment of malondialdehyde levels and total antioxidant status.
Results: Group I had 50 males and 40 females and group II had 45 males and 45 females. The mean malondialdehyde (MDA) level in group I was 3.68 µM and in group II was 1.90 µM. The mean total antioxidant status (TAS) in group I was 0.48 mM and in group II was 1.68 mM in group II. The difference was significant (P< 0.05).
Conclusion: Type II diabetes mellitus patients exhibited decreased TAS status and increased MDA levels. It may be considered as an early marker of the pathogenesis of complications in type 2 diabetes mellitus.

Lipid peroxidation products are capable of inhibiting the activity of enzymes of glycolysis and oxidative phosphorylation, inhibiting the synthesis of protein and nucleic acids, inhibiting many membrane-bound enzymes and thus exerting a pronounced damaging effect on cells and the body as a whole. It is of particular interest to study the intensity of lipid peroxidation in the body during myocardial infarction, which was the price of this study.