Online ISSN: 2515-8260

Keywords : Female Infertility

Biochemical and endocrinological aspects of female infertility: Descriptive comparative study

Dr. Arun William, Dr. Dinesh Roy D, Dr. Viji Krishnan

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 2, Pages 2038-2043

Infertility is a disease of the reproductive system and its treatment can affect all aspects of people’s lives, which can cause various psychological-emotional disorders or consequences including frustration, hopelessness, depression, guilt, anxiety and feelings of worthlessness in life. In order to evaluate Biochemical and endocrinological aspects of female infertility, a test-control study was designed. For the study 150 clinically diagnosed infertile female subjects and 150 age matched healthy females with one or more children were involved in the study as control. Level of uric acid concentration was comparatively higher among test group (5.5mg/dL) than the control subjects (4.4mg/dL) (p<0.005). The inflammatory marker (hsCRP) demonstrated a very high concentration among the test subjects than the control group (1.0±0.59) (p<0.005). It was evident that, study group expressed an FSH concentration of 30±10 and control with a FSH level of 12.8±6.00 (p=<0.005).


Dr. Monika Shukla, Dr. Monika Puranik, Dr. Swapnil Puranik, Dr. Krati Khandelwal, Dr. Megha Jain, Dr. Pranav K Dave

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 11463-11474

Background: Female infertility is quite prevalent these days and with development of newer treatment options, Magnetic Resonance Imaging (MRI) is frequently performed where diagnosis is arduous. The aim of our research was to study the indications of MRI pelvis and its accuracy with respect to hysteroscopy/laparoscopy in evaluation of female infertility and to aid in determination of cases requiring interventional or surgical correction.
A retrospective cross sectional study was carried out for two years eight months between March2019 and November 2021. Amongst 183 infertile women(with primary and secondary infertility) of age group 19-40 years were evaluated, 82 patients who were not conclusively diagnosed by Ultrasonography (USG) or Hysterosalpingography (HSG) were referred for MRI.The MRI findings were compared with hysteroscopic/laparoscopic findings which were considered final. Data was analyzed for indications of MRI and its diagnostic accuracy in female infertility.
Results: On MRI, we found 27 cases of Mullerian anomalies, 16 cases of leiomyoma(Multiple/submucosal), 12 cases of adenomyosis , 11 cases of infective etiology [pelvic inflammatory disease (PID) / pyosalpinx/ hydrosalpinx],  9 cases of endometriosis, 3 cases of endometrial polyp, 3 cases of uterine synechiae. MRI was able to correctly diagnose 78 out of 82 cases. Our MRI findings were confirmed with hysteroscopy or laparoscopy. In majority of cases, MRI findings were same as the surgical findings. Only in few(n=3)cases, findings onMRI were discordant with final surgical diagnosis.
Conclusion: Our study shows that MR imaging is highly accurate noninvasive method of choice for diagnosis of uterovaginal & tubo-ovarian lesions associated with female infertility, especially where the diagnosis is controversial on USG and HSG. Its diagnostic accuracy approaches that of hysteroscopic/ laparoscopic findings in 96.2% of the cases. The superior soft tissue resolution and multiplanar capability of MRI helps in better delineation of the morphology and orientation of pelvic structures. Hence MRI plays a decisive role in management of female infertility and can come to rescue in complex cases.

Screening of CD8 Gene in Females with Idiopathic Infertility: A Cross Sectional Study in Thi-Qar

Enaas S. Jawad; Muslim N. Saeed

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 38-42

Background: Infertility affects a major proportion of the individuals in the reproductive
age group. About 40-50% of infertile cases are contributed by the female factors.
Several cases of female infertility remain unexplained. There are several genetic factors
associated with female infertility. Among these genetic factors, the most important
factors are gene mutations, chromosomal abnormalities, and epigenetic factors. There
are several single gene mutations reported in female infertility. The present study was
conducted to screen the CD8 gene in unexplained cases of female infertility in the Thi-
Qar province.
Material and methods: In the cross sectional, comparative study conducted over the
year of 2018, 42 females were recruited. Among the 42 females, 11 were diagnosed to
have primary infertility, 11 had secondary infertility, and 20 were healthy fertile controls.
Venous blood was collected in EDTA coated vials by venipuncture. DNA was extracted
and the CD8 gene was PCR amplified and sequenced to screen for any mutations.
Result: Only 1 out of 22 patients (4.55%) showed a sequence change in the CD8 gene.
This patient showed one transition mutation (C418T) and one transversion mutation
(T419G). These mutations were absent in the controls.
Conclusion: Our study did not confirm the role of CD8 gene mutations in idiopathic
female infertility. However, further extensive studies should be conducted to
understand the role of CD8 gene mutations in idiopathic female infertility.