Online ISSN: 2515-8260

Keywords : Hepatitis C virus

Prevalence of Hepatitis B and C in Thi-Qar Province - Iraq from 2015-2019

Rana A. Othman; Yahya A. Abbas

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 43-48

Background: Hepatitis B virus and hepatitis C virus are two of the most commonly
transmitted infectious agents by Blood transmitting so it is still remains a considerable
global health problem, this prospective cross-sectional study was conducted
between 2015 and 2019 at the directorate of main Blood Bank, dialysis center,
thalassemia center and public health laboratory in Thi-Qar Province -Iraq, during that
period a total of 1323 patients, 948 (71.7%) males and 375 (28.3%) females, they
suffering from signs and symptoms of liver diseases.
The aim of study: There were no adequate epidemiological studies on the prevalence
of viral hepatitis, especially in last years and there were no national solutions to limit
it’s, we conduct this study to determine the prevalence of both types at Thi-qar
Sampling and methods: This study was conducted in January 2015 to December 2019,
on main blood bank, patients referred to the thalassemic center, renal dialysis unit and
public health laboratory at Thi-Qar province, the study was conducted on 176848
individuals, from those (1323) cases, (515) blood donors, (45) thalassemic patients, (91)
renal failure patients and public health laboratory (672), the following data were
recorded: Age, sex, date, resident, type of infection, data were collected by from
statistical units. Data were analyzed using SPSS for windows (version 23.0, SPSS lnc,
Chicago, III), for comparison between groups as appropriate; P ≤ 0.01 was considered
statistically significant.
The results: The results of infection were found 1323(0.7%), its distributed as :672
(50.8%) were public health laboratory patients, 515 (38.9%) blood donors, 91 (6.9%)
renal dialysis patients and 45(3.4%) thalassemic patients, the results was showed
higher infection in males more than females, with high prevalence in age groups (31-
40) and (41-50) years, with significant difference (P<0.01) .
Conclusions: despite the findings revealed that Thi-Qar is acceptable percentage rate
of prevalence of infection, but may be increased the infection by Immigration from
endemic areas, especially for business , transfusion of blood, a major roots of
infections by renal dialysis, thalassemic patients were a risk groups because
administration of blood.

Assessed Valuate of Soluble Programmatic Cell Death Ligand 1 (PD-1) in Sera of Hepatitis C Virus in Iraqi Patients

Seenaa Wdaah AlSalih; Alaa Raisan Rashid; Syakirah Samsudin; Moatasem AlSalih

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 57-63

Objective: The present study aims at detecting the concentration of human PD-1 in
samples taken from hepatitis C virus patients related to the healthy control group which
studying the relation between viral load associated with (PD-1) concentration.
subject of the treatment group content from 68 samples which is selected randomly
from the patients with HCV, subdivide into 32 males and 36 females aged 23-76 years
with 30 healthy individuals divided 20 males and 10 females. During first April to end of
June 2020.
ELISA kit from (Shanghai Yehua Biological Technology Company, China) was used to
measure programmed death concentration. While Real time-PCR technique (Device
Smart Cycler, USA) was used for calculating the viral load, according to Sacace
Biotechnology kit. The result was a high concentration of PD-1 in patients 295.709±29.36
and 168.337 ± 80.906) compared to healthy (106.014±63.90, 110.176 ± 36.681).
Respectively, and significant difference. and we found that PD1 concentration was
directly proportional to viral load
Conclusion: We have found that there is an enhancement in PD-1 concentration in
patients associated with healthy control groups, and We also found that PD1
concentration was directly proportional to viral load, Whenever, increased the viral load,
had risen the PD 1 concentration.

Hepatitis C Seroprevalence Among A Tertiary Hospital Based General Population In Northern India

Heena Sharma; Vasim Mahdi Zaidi; Dr.Gomty Mahajan; Suman Kumari

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 4088-4094

Hepatitis C is a disease caused by enveloped, RNA virus belongs to Flaviviridae family. It causes inflammation of the liver that lead to liver cirrhosis and finally hepatocellular carcinoma. Its genome consists of open reading frame (ORF) codes for structural and non-structural proteins. The ORF have 5´ and 3´ UTR regions. Hepatitis C virus is cause of post transfusion hepatitis. Symptoms of HCV include fatigue, dark urine, belly pain, joint pain, itchy skin, sore muscles and jaundice. It is a blood-borne transmitted agent. Use of unsafe therapeutic injections also leads to HCV infection. Diagnosis of HCV infection can be done by various methods like Enzyme Immunoassay (EIA) and Recombinant Immunoblot Assay (RIBA). The aim of this study is to estimate seroprevalence of Hepatitis-C in both sexes and different age groups in hospital based general population. And to study the trends of HCV infections in a tertiary hospital located at Northern India.
A prospective study was conducted for four months (January-April) at Tertiary Hospital in Northern India. Total numbers of 1643 blood samples were screened for the presence of anti-HCV antibodies in patient’s serum. Samples were tested by HCV TRI-DOT rapid test. Positive samples were retested by SD BIOLINE HCV rapid test and confirmed by ELISA.Out of 1643, 102 (6.2%) samples were HCV positive. Among seropositive samples, 48 were males (2.9%) and 54 were females (3.2%). HCV seropositivity was shown by 40 IPD patients (2.4%), 52 OPD patients (3.1%) and 10 ICU patients (0.6%). Among departments, patients from Recovery showed (0.6%), General ward (1.0%), Private room (0.2%), Neurology lab (0.1%) and Emergency (0.3%) showed HCV seropositivity. On analyzing age-wise seropositivity, it was found that maximum seropositivity was seen in 30-40 years (2.9%) followed by >55 years (1.4%), 40-55 years (1.2%) and<30 years (0.5%). Analysis of patients on the basis of risk factors showed that 17 had history of surgical operation (1.0%), 18 had history of blood transfusion (1.0%), 6 had history of dental procedure (0.3%), 25 showed history of injecting drug use (1.5%), 21 used contaminated syringes (1.2%) and 15 patients was under haemodialysis (0.9%). Professional health worker should protect themselves while handling infected blood. Counselling and testing should be done for those who are at risk for infection.