Online ISSN: 2515-8260

Keywords : period


MORPHOFUNCTIONAL ACTIVITY OF NEUROSECRETER CELLS IN THE ARCUATIC NUCLEUS OF HYPOTHLAMUS DURING THE PERIOD POST-REANIMATION DISEASE

Karabayev Aminjon Gadaevich; Yusupov Mirzo Murodovich; Ismatova Marguba Shaukatovna; Nurimov Paxlavon Baxtiyorovich; Urokov Gayrat Mardikulovich

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 948-953

In the postresuscitation period through the I-II-III-IV-V-stages and long-term periods (1 and 3 months) of postresuscitation illness, when modeling a 10-minute clinical death, we studied the relationship between the reactivity of the autonomic nervous system and the morphofunctional activity of neurosecretory cells of the arcuate nucleus of the hypothalamus of white mature rats - males, weighing 180-220 grams, who underwent a 10-minute arrest of systemic circulation (clamping of the vascular bundle of the heart according to the method of V.G. Korpachev). Sham-operated animals served as control. Physiological, morphological, morphometric, histochemical, cytophotometric studies of the state of reactivity of the autonomic nervous system and morphofunctional activity of neurosecretory cells of the arcuate nucleus of the hypothalamus were carried out. We used image analysis using a Leiss microscope with an electronic micro-attachment connected to an Intel computer, and the content of neurosecretory substances was studied using a cytophotometer. In the postresuscitation period, during stage I, hyperhydration of the cytoplasm of neurosecretory cells of the arcuate nucleus of the hypothalamus was revealed against the background of the predominance of the tone of the parasympathetic nervous system. During stage II of the predominance of the tone of the parasympathetic nervous system, starting from stage III of the disease, against the background of the predominance of the tone of the sympathetic nervous system, hyperhydration of the nuclei of neurosecretory cells of the arcuate nucleus of the hypothalamus is observed with an increase in the release of neurosecret into the blood, i.e., compensatory-adaptive reactions were observed. Compensatory-restorative processes with a shift towards depletion of neurosecretory cells of the arcuate nucleus were observed starting from stage IV and in the remote periods of postresuscitation disease against the background of the predominance of the tone of the sympathetic nervous system and overhydration of the nuclei.

Reactivity of the supraoptic, arcuate nucleus of the hypothalamus and the B- and D-basophilic cells of the adenohypophysis in the early postreanimation period

Karabayev Aminjon Gadaevich; Nurimov Paxlavon Baxtiyorovich; Urokov Gayrat Mardikulovich; Bobokandova Mexriniso Fazliddinovna; Xasanova Sabina Baratovna.

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 954-957

In the early postresuscitation period, we studied the relationship of neurosecretory cells of the supraoptic nucleus and paraventricular nucleus with the B and D basophilic cells of the adenohypophysis in the early stages (I-II-III stages) of postresuscitation disease in animals that underwent a 5-minute clinical death by clamping the vascular bundle of the heart using method B G. Korpacheva (1982). Sham-operated animals served as control. Physiological, morphological, morphometric, histochemical studies and morphofunctional activity of neurosecretory cells of the supraoptic and arcuate nuclei of the hypothalamus, as well as Band D-basophilic cells of the adenohypophysis were carried out. We used image analysis using a Leiss microscope with an electronic micro-attachment connected to an Intel computer, and the content of neurosecretory substances was studied using a cytophotometer. In the early postresuscitation period (stages I-II), there is an increase in morphofunctional activity, which is more pronounced in the NSC SOY, ARN and D-basophilic cells of the adenohypophysis, at the III-stage post-resuscitation disease, there is a further increase in the morphofunctional
activity of NSC SOS ARN and B-basophilic cells adenohypophysis, with a shift towards depletion.