Online ISSN: 2515-8260

Keywords : PD-1

Assessed Valuate of Soluble Programmatic Cell Death Ligand 1 (PD-1) in Sera of Hepatitis C Virus in Iraqi Patients

Seenaa Wdaah AlSalih; Alaa Raisan Rashid; Syakirah Samsudin; Moatasem AlSalih

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 57-63

Objective: The present study aims at detecting the concentration of human PD-1 in
samples taken from hepatitis C virus patients related to the healthy control group which
studying the relation between viral load associated with (PD-1) concentration.
subject of the treatment group content from 68 samples which is selected randomly
from the patients with HCV, subdivide into 32 males and 36 females aged 23-76 years
with 30 healthy individuals divided 20 males and 10 females. During first April to end of
June 2020.
ELISA kit from (Shanghai Yehua Biological Technology Company, China) was used to
measure programmed death concentration. While Real time-PCR technique (Device
Smart Cycler, USA) was used for calculating the viral load, according to Sacace
Biotechnology kit. The result was a high concentration of PD-1 in patients 295.709±29.36
and 168.337 ± 80.906) compared to healthy (106.014±63.90, 110.176 ± 36.681).
Respectively, and significant difference. and we found that PD1 concentration was
directly proportional to viral load
Conclusion: We have found that there is an enhancement in PD-1 concentration in
patients associated with healthy control groups, and We also found that PD1
concentration was directly proportional to viral load, Whenever, increased the viral load,
had risen the PD 1 concentration.

Study the role of cytokines (IL-6 and TGFᵦ-1) and apoptosis-related proteins (PD-1 and STAT3) in the outcome of HCV infection in Wasit Province/Iraq

Nada M. Sadoon; Kadhum J. Gattia; Ahmed D. Jabbar

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 4361-4378

Hepatitis C virus is one of the public health disorders in the world. It’s blood-borne, causes acute disease and may extend to chronic infection, if HCV is untreated, it can progress and lead to fibrosis, cirrhosis and cancer of the liver and then death. Since the virus targets the immune system, here in this finding two cytokines (IL-6 and TGFᵦ-1) and two apoptosis-related proteins (PD-1 and STAT3) were tested serologically by Enzyme Linked-Immunosorbant Assy (ELISA) technique to detect the changes in their levels during the infection. The results revealed that IL-6 and TGFᵦ-1were abnormally raised in patients with HCV compared with healthy peoples (P<0.001). Apoptosis-related proteins involved in this study were PD-1 and STAT3, both were also elevated in HCV patients rather than control (P<0.001). The age group (46-55) of patients was the most group displayed higher levels in IL-6, TGFᵦ-1, PD-1 and STAT3. In conclusion, IL-6 was good indicator on the liver inflammation during HCV, TGFᵦ-1stimulates the transformation of the infected hepatocytes to be fibrotic, cirrhotic or cancer cells. PD-1 considered as a checkpoint for the impairment of T cell during infection, continuous stimulation of STAT3 by IL-6 and other factors contribute to HCV progression.