Online ISSN: 2515-8260

Keywords : ELISA


Serodiagnosis of Human Herpesvirus-8 among Iraqi Blood Donors

Zainab B. Mohammed; Shatha F. Abdullah

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 69-74

Human herpesvirus type 8 (HHV-8) or known as Kaposi sarcoma-associated herpesvirus
(KSHV) is the etiologic agent for all clinic-epidemiological forms of Kaposi’s sarcoma (KS).
Many studies have been documented that blood transfusion plays an important role in
HHV-8 transmission. This study was prepared to determine the frequency of HHV-8
antibodies among blood donors. A cross-sectional study was conducted on ninety blood
donors who attended the Iraqi National Centre for Blood Transfusion in February 2019 to
detect HHV-8 IgG antibodies using an enzyme-linked immunoassay method. Anti-HHV8-
IgG were detected in 78 out of 90 (86.6%) serum samples. A significant association has
been found between anti-HHV-8 detection and associated risk factors in blood donors such
as sexual relationships (legal and illegal), occupation, surgical and dental operations, blood
transfusion, cupping, tattooing, smoking and numbers of blood donation. Our results
confirmed that a high percentage of HHV8-IgG among blood donors may indicate an
increased threat to HHV-8 infection via blood transfusion.

Determination of levels of Cytokines (HGF ,TNF- ,CXCL-13) in serum of hepatitis B patients in Wasit Province

Sabreen Hadi Hanash; Kareem Hamed Ghali; Ahmed DarwishJabbar

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 4279-4294

HBV infection is one of the major concerns and a constant threat to public health. Cytokines are used in biological studies as vital signs in the diagnosis, treatment, and follow-up of progression a number of diseases such as CHB and HCC . This study aimed to evaluation the levels of HGF , TNF- α , CXCL-13 in HBV patients .Studies have shown that the hepatocyte growth factor is the engine for the development of HCC caused by infection with HBV, and may serve as an effective biomarker for targeted therapy. The study included 100 patients with positive HBs Ag (100% positive). Three cytokines (HGF , TNF- α , CXCL-13) were analyzed in the
serum of patients ,and the results showed highly significant increase inthe levels of those cytokines in patients comparing to healthy individuals(p≤0.01). Also ,the results showed there is a highly significant difference(P<0.001) of HGF levels when comparing between acute andchronicHBV patients .Our results concluded HGF , TNF- α , CXCL-13 play a key role in development of HBV.