Online ISSN: 2515-8260

Keywords : ELISA

IgE Reactivity to Latex in sera of Indian Patients: An Original Research

Dr. Heena Tiwari; Dr. Harshada Inamdar; Dr. Rahul VC Tiwari; Dr. Anil Managutti; Dr Rakhi Bharat; Dr. J. Sreedevi

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 11, Pages 5936-5941

Aim: The purpose of our research was to assess and analyse IgE cross-reactivity in Indian patients sera in relation to latex extract.
Methodology: Sera from 27patients with latex allergy and control sera from nonsensitive individuals were studied for latex-specific IgE antibodies. Two antigen preparations were used from different rubber tree saps. All patients had skin prick test results that were positive to latex antigens, and all sera were evaluated by enzyme-linked immunosorbent assay (ELISA) with the various antigens.
Results: There were considerable differences in the reactivity of patient sera with the different antigens. Only 50% of the sera from patients with later allergy demonstrated significant levels of IgE to later as determined by enzyme-linked immunosorbent assay.
Conclusion: The results indicate that reagents such as rubber tree sap, which contain multiple clinically significant antigenic components, should be included in evaluation of latex allergy and that differences in patient populations may result in serologic variances

Equine Chorionic Gonadotropin (eCG) Polyclonal Anti Body Production in Madura Bulls for Estrus Synchronization and Superovulation Program in Ruminants

Herry A. Hermadi; Sunaryo H. Warsito; Erma Safitri

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 10, Pages 1263-1269

This study aimed to produce polyclonal anti-body (Abpo) equine chorionic gonadotropin (eCG) in Madura bulls. The Abpo-eCG was an alternative hormone for the superovulation technique and estrus synchronization of ruminant. The isolation of sera eCG derived from local Indonesian pregnant mare sera at 1-3 months age had beed collected from jugular veins, and then purification with charcoal was done by chromatography Sephadex G100. After that, the produce of Abpo eCG have done in two Madura bulls carried out injected with 3,000 IU eCG from local Indonesian pregnant mare sera 3 times at 10 day intervals. Furthermore, Abpo eCG was isolated from serum taken from the jugular vein of bull, then extraction was done by adding absolute charcoal and ethanol and centrifuged at 3000 rpm for 15 minutes at 4oC. Next step, the identification of Abpo-eCG that producted from Madura bull by SDS-PAGE 12% method, Western Blot and Elisa indirect to find out the highest level at the 7th week of 408.50 mlU /mL. Then purified by CM Sephadex G-100 coloumn chromatography technique. The final product was made as a Frozen Dry dosage form.

Serodiagnosis of Human Herpesvirus-8 among Iraqi Blood Donors

Zainab B. Mohammed; Shatha F. Abdullah

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 69-74

Human herpesvirus type 8 (HHV-8) or known as Kaposi sarcoma-associated herpesvirus
(KSHV) is the etiologic agent for all clinic-epidemiological forms of Kaposi’s sarcoma (KS).
Many studies have been documented that blood transfusion plays an important role in
HHV-8 transmission. This study was prepared to determine the frequency of HHV-8
antibodies among blood donors. A cross-sectional study was conducted on ninety blood
donors who attended the Iraqi National Centre for Blood Transfusion in February 2019 to
detect HHV-8 IgG antibodies using an enzyme-linked immunoassay method. Anti-HHV8-
IgG were detected in 78 out of 90 (86.6%) serum samples. A significant association has
been found between anti-HHV-8 detection and associated risk factors in blood donors such
as sexual relationships (legal and illegal), occupation, surgical and dental operations, blood
transfusion, cupping, tattooing, smoking and numbers of blood donation. Our results
confirmed that a high percentage of HHV8-IgG among blood donors may indicate an
increased threat to HHV-8 infection via blood transfusion.

Determination of levels of Cytokines (HGF ,TNF- ,CXCL-13) in serum of hepatitis B patients in Wasit Province

Sabreen Hadi Hanash; Kareem Hamed Ghali; Ahmed DarwishJabbar

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 4279-4294

HBV infection is one of the major concerns and a constant threat to public health. Cytokines are used in biological studies as vital signs in the diagnosis, treatment, and follow-up of progression a number of diseases such as CHB and HCC . This study aimed to evaluation the levels of HGF , TNF- α , CXCL-13 in HBV patients .Studies have shown that the hepatocyte growth factor is the engine for the development of HCC caused by infection with HBV, and may serve as an effective biomarker for targeted therapy. The study included 100 patients with positive HBs Ag (100% positive). Three cytokines (HGF , TNF- α , CXCL-13) were analyzed in the
serum of patients ,and the results showed highly significant increase inthe levels of those cytokines in patients comparing to healthy individuals(p≤0.01). Also ,the results showed there is a highly significant difference(P<0.001) of HGF levels when comparing between acute andchronicHBV patients .Our results concluded HGF , TNF- α , CXCL-13 play a key role in development of HBV.

Hepatitis C Seroprevalence Among A Tertiary Hospital Based General Population In Northern India

Heena Sharma; Vasim Mahdi Zaidi; Dr.Gomty Mahajan; Suman Kumari

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 4088-4094

Hepatitis C is a disease caused by enveloped, RNA virus belongs to Flaviviridae family. It causes inflammation of the liver that lead to liver cirrhosis and finally hepatocellular carcinoma. Its genome consists of open reading frame (ORF) codes for structural and non-structural proteins. The ORF have 5´ and 3´ UTR regions. Hepatitis C virus is cause of post transfusion hepatitis. Symptoms of HCV include fatigue, dark urine, belly pain, joint pain, itchy skin, sore muscles and jaundice. It is a blood-borne transmitted agent. Use of unsafe therapeutic injections also leads to HCV infection. Diagnosis of HCV infection can be done by various methods like Enzyme Immunoassay (EIA) and Recombinant Immunoblot Assay (RIBA). The aim of this study is to estimate seroprevalence of Hepatitis-C in both sexes and different age groups in hospital based general population. And to study the trends of HCV infections in a tertiary hospital located at Northern India.
A prospective study was conducted for four months (January-April) at Tertiary Hospital in Northern India. Total numbers of 1643 blood samples were screened for the presence of anti-HCV antibodies in patient’s serum. Samples were tested by HCV TRI-DOT rapid test. Positive samples were retested by SD BIOLINE HCV rapid test and confirmed by ELISA.Out of 1643, 102 (6.2%) samples were HCV positive. Among seropositive samples, 48 were males (2.9%) and 54 were females (3.2%). HCV seropositivity was shown by 40 IPD patients (2.4%), 52 OPD patients (3.1%) and 10 ICU patients (0.6%). Among departments, patients from Recovery showed (0.6%), General ward (1.0%), Private room (0.2%), Neurology lab (0.1%) and Emergency (0.3%) showed HCV seropositivity. On analyzing age-wise seropositivity, it was found that maximum seropositivity was seen in 30-40 years (2.9%) followed by >55 years (1.4%), 40-55 years (1.2%) and<30 years (0.5%). Analysis of patients on the basis of risk factors showed that 17 had history of surgical operation (1.0%), 18 had history of blood transfusion (1.0%), 6 had history of dental procedure (0.3%), 25 showed history of injecting drug use (1.5%), 21 used contaminated syringes (1.2%) and 15 patients was under haemodialysis (0.9%). Professional health worker should protect themselves while handling infected blood. Counselling and testing should be done for those who are at risk for infection.

Comparative Study Of Rheumatoid Factor - Igm Autoantibody Testing By Latex Agglutination Nephelometry And Elisa In Patients With Rheumatoid Arthritis

Dr.C. Devi; Dr.R. Ravichandran; Dr. Logeswari Selvaraj; Dr.S. Ramesh; Dr.T. Aarthipriya

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 8, Pages 4521-4527

Objectives: To test Rheumatoid factor (RF) IgM autoantibody in Patients with
Rheumatoid Arthritis by various methods like latex agglutination, Nephelometer and
ELISA. Comparative analysis of the sensitivity and specificity of the tests performed.
Materials and Methods: The study was conducted for a period of six months from June
2018 to November 2018 in a tertiary care hospital in Chennai. 90 patients attending
Rheumatology OPD or admitted in the ward with the diagnosis of Rheumatoid arthritis,
satisfying the inclusion and exclusion criteria were taken up for the study.
Inclusion criteria: Clinically diagnosed Rheumatoid Arthritis patient as per revised ACR
1987 classification criteria. Duration of symptoms (1yr- early (RA) 1 Yr. (Established RA).
Exclusion Criteria: Those with systemic connective tissue diseases like SLE, Scleroderma,
MCTD, Sjogren syndrome, those with chronic liver diseases, tuberculosis, subacute
Bacterial endocarditis, Pregnancy, Lympho reticular malignancies are excluded for the
study. Those with onset 16 years of age are also excluded. Under aseptic precautions about
3ml of blood was collected from each Patient. Rheumatoid factor (RF) IgM was tested for
each patient by all three methods Latex agglutination, Nephelometry and ELISA.
Results: IgM rheumatoid factor (RF) was detected in the sera of 90 patients with
Rheumatoid Arthritis. The percentage of positivity for Rheumatoid factor by Latex
agglutination, ELISA and Nephelometry were 41,64&60 respectively. Sensitivity and
Specificity of ELISA when compared to nephelometry were 63 & 33% followed by latex
agglutination 41 &59%.
Conclusion: Though nephelometry is considered as gold standard, in this study ELISA was
highly sensitive more even than nephelometry in Rheumatoid factor detection followed by
latex agglutination.