Online ISSN: 2515-8260

Keywords : IL-6

Association of IL-4 and IL-6 Gene Polymorphism with Pulmonary Tuberculosis

Nktel Faaz AL-Saad; Mohammed A.K Alsaadi; Ali Hmood Al-saadi

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 75-81

The case-control study was aimed to evaluate the effect of IL-4 and IL-6 gene
polymorphism on susceptibility to pulmonary tuberculosis in patients in Babylon province
and also measure the serum level of these cytokine in in sera of studied group. The
genotype association of rs2243250 (C/C, C/T, T/T) were significant (P<0.05) with (46.7,
38.3, 15)% respectively for PTB cases and (36.7, 26.6, 36.7)% respectively for control
group. Allele's frequency for patients and control for IL-4 (rs2243250) were also
significant (P<0.05) and the C allele represent the risk allele for PTB patients with odd
ratio (95% CI) of 1.93(1.15-3.24), while the T allele represent protective allele for control
group 0.52 (0.31-0.87). The genotype association of rs1800796 (C/C, G/C, G/G) were
significant (P<0.05) with (43.3, 38.3, 18.4)% respectively for PTB cases and (50, 15, 35)%
respectively for control group, whereas, the allele's frequency for patients and control of
IL-6 (rs1800796) were insignificant with (P>0.05). There was a significant difference in
mean level of both IL-4 and IL-6 serum concentration between pTB patients and controls
(P<0.01). Also, there were an insignificant effect of IL-4 (rs2243250) and IL-6 (rs1800796)
gene polymorphism on the difference in serum level of both IL-4 and IL-6 respectively in
studied groups (P>0.05).

Anti-Inflammatory and Gene Expression Studies of Terminalia chebula Extracts against Interleukins and TNF

Mustafa M. Farhan; Saeed Arrak Turkey; Nadhim M.H .

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 229-237

Aims: Since ages, several plant extracts and Ayurvedic formulations were used to treat
ailments and such studies were well documented in the recent decades. Even some of
the plants were screened for their efficacy as immunomodulators to restore and
rejuvenate the immune system. The present study deals in screening for the possible
healing effects of Terminalia chebula on IL-2 and IFN-γ levels.
Methods: The raw and dried fruits of the sample were pulverized finely and extracted with
methanol. Following which their aqueous solutions are reextracted with hexane, ethyl
acetate and chloroform to study the possible cytotoxic effects. Lipopolysaccharide (LPS)-
stimulated macrophage cells were used throughout the study to measure the effect of
extracts on nitric oxide (NO) production using Griess method. Expression of
Cyclooxygenase-2 (COX2) and tumor necrosis factor (TNF)-α were studied by real time
PCR quantification along with estimation of IL-1β and IL-6 cytokine levels using the
enzyme-linked immunosorbent assay (ELISA).
Results: The chloroform extract showed maximum NO inhibition at about 18.31±
1.6μmol/L. In accordance to the above result, COX2 and TNF-α were found to be
downregulated by 12 and 7 fold respectively at 100μg/mL (P < 0.005). Chloroform extract
significantly reduced IL-1β levels to 21.23 ± 0.21pg/ml (100μg/mL) and also lowered the
levels of IL-6 to 45.67± 3.31pg/ml (100μg/ml)
Conclusion: The present study confirmed the positive effect of the chloroform extract in
reducing the NO secretion and also by showing an inhibition in the expression of COX2,
IL-1β, IL-6, and TNF-α. Thus to conclude Terminalia chebula could be used as the best antiinflammatory
candidate drug in addition to the many chemical compounds available in the
medical markets.

Study the role of cytokines (IL-6 and TGFᵦ-1) and apoptosis-related proteins (PD-1 and STAT3) in the outcome of HCV infection in Wasit Province/Iraq

Nada M. Sadoon; Kadhum J. Gattia; Ahmed D. Jabbar

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 4361-4378

Hepatitis C virus is one of the public health disorders in the world. It’s blood-borne, causes acute disease and may extend to chronic infection, if HCV is untreated, it can progress and lead to fibrosis, cirrhosis and cancer of the liver and then death. Since the virus targets the immune system, here in this finding two cytokines (IL-6 and TGFᵦ-1) and two apoptosis-related proteins (PD-1 and STAT3) were tested serologically by Enzyme Linked-Immunosorbant Assy (ELISA) technique to detect the changes in their levels during the infection. The results revealed that IL-6 and TGFᵦ-1were abnormally raised in patients with HCV compared with healthy peoples (P<0.001). Apoptosis-related proteins involved in this study were PD-1 and STAT3, both were also elevated in HCV patients rather than control (P<0.001). The age group (46-55) of patients was the most group displayed higher levels in IL-6, TGFᵦ-1, PD-1 and STAT3. In conclusion, IL-6 was good indicator on the liver inflammation during HCV, TGFᵦ-1stimulates the transformation of the infected hepatocytes to be fibrotic, cirrhotic or cancer cells. PD-1 considered as a checkpoint for the impairment of T cell during infection, continuous stimulation of STAT3 by IL-6 and other factors contribute to HCV progression.