European Journal of Molecular & Clinical Medicine

Relevance: The problem of genital prolapse, despite such a long history of the
disease, still does not have a definite solution. Currently, the problem of genital
prolapse in women remains relevant due to the high impact on the social part of life.
Genital prolapse and impaired function of neighboring organs significantly reduce the
quality of life. Every year there are more and more articles linking genital prolapse with
connective tissue pathology and with a change in collagen structure. This paper
describes the relationship of polymorphisms of the collagen genes COL1A1 and
COL3A1 and pelvic organ prolapse in women. This study aimed to analyze the effect of
rs1800012 and rs1107946 polymorphisms of the COL1A1 gene, rs1800255 of the
COL3A1 gene on the development of pelvic organ prolapse in women of the Belgorod
region.
Material and Methods: In 2018 we analyzed 268 women who were residents of the
city of Belgorod and the Belgorod region, among which 2 groups were identified.
Group 1 (N = 53) included women who had signs of pelvic organ prolapse. Group 2 (N
= 215) consisted of women with an objectively proven absence of prolapse. The study
was carried out in the gynecological department of Belgorod Regional Clinical Hospital
of St. Joasaph.
Results: In our study, it was found that the frequencies of polymorphisms of the
COL1A1 and COL3A1 genes in residents of the Belgorod region did not differ from
those in representatives of European populations. Thus, in the studied population of
Belgorod women, the allele A of the rs1800255 polymorphism of the COL3A1 gene
was characterized by a protective effect in relation to the predisposition to pelvic organ
prolapse.
Conclusion: In our study, a high association of rs1800255 polymorphism of the
COL3A1 gene to pelvic organ prolapse in women of the Belgorod region was
confirmed. At the same time, no connection between the rs1800012 and rs1107946
polymorphisms of the COL1A1 gene and genital prolapse was detected.

areca nut association with human being is since pre vedica period where it is used as a regular part of meals also it is considered as a token of gift in many cultures. Its deep association with traditions of our country we are not able to completely eradicate its use. Areca nut has its systemic effects on almost every system of our body. Thus its use should be curbed. Apart from its deleterious effects scientists have progressed to utilise its constituents as a treatment modality for cancers.

From the aboveground and root parts of Rumex confertus plants, the method
of triple ethanol extraction was used to extract the polyphenol tannic compounds extract -
ERc and evaluate its biological activity. It was established that ERc with a single oral
administration at a dose of 5000 mg/kg does not cause toxic effects in experimental
animals and their death, with prolonged oral administration at a dose of up to 500 mg/kg
also does not cause animal death and pathological changes in their organs. At
concentrations of 50-100 μg/ml, it inhibited the growth of Bacillus subtilis, Pseudomonas
aeruginosa and Staphylococcus aureu. At a concentration above 100 μg/ml, it showed a
cytotoxic effect on normal fibroblast cells, and at a concentration of 50 μg/ml ERc
significantly stimulated the proliferation of these skin cells, which allowed us to choose
this concentration as effective and introduce it into a collagen based RP. As a result, the
obtained RP under the name СC-Rc accelerated the time of complete epithelialization of
wounds by 1.5 times. At the same time, the commercial NeuSkin-F comparison drug
(Eucare Pharmaceuticals (P) Limited, India) consisting of collagen without drug additives
showed very low wound healing properties in our studies due to poor adhesion. In this
regard, our CC-Rc, which has significantly better congruence and adhesion to the wound,
showed an excellent result, did not require additional fixation.