Online ISSN: 2515-8260

Keywords : RADP typing


Genotyping Diversity of Proteus vulgaris by using RAPDPCR and Study of Some Factors on the Activity of Bacteria

Lamees A. Abdul-Lateef

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 182-193

P. vulgaris causes variety of infection such as, UTIs, wound infections, burn infections,
bloodstream infections, and respiratory tract infections, bacteremia and brain abscesses. A
total 120 specimen, were collected from clinical samples (urine, vagina, wound, and burn).
only 18 isolates of Proteus vulgaris were recovered. The relationship between 18 Proteus
vulgaris isolated using RAPD-PCR was performed. The ten primer showed polymorphism
among the isolates generating 86 bands, 54 of which were polymorphic with sizes ranging
between 150-30000bp. The mean percentage of ten primer 62.79%. Proteus
vulgaris isolates identified were screened for biofilm production by TCP method. Among
the isolates, 55.55% were biofilm producer and 44.44% were non/weak biofilm producer.
Regard the effect of NAC (10%) on biofilm formation was also studied, this study proved
that NAC effectively reduced the viability of biofilm formation from (55.55) to (5.55%). On
the other hand, the effect of NAC and green tea at concentration (0, 1, 5, 10, and 15
mg/mL) on urease was also investigated, it was state the high concentration of it could
cause inhibition to urease production