Online ISSN: 2515-8260

Keywords : Escherichia coli


Dr. Swetha Munivenkatappa, Dr. Vasavi Mounika Sampathi, Dr. Srinivas MG, Dr. Kandregula Pavani

European Journal of Molecular & Clinical Medicine, 2023, Volume 10, Issue 2, Pages 1040-1047

Asymptomatic bacteriuria (ASB) is common during pregnancy and must be identified and treated to prevent poor maternal and obstetric outcomes. This study aims to determine the prevalence of asymptomatic bacteriuria among pregnant women in our antenatal clinic. The study also looks at any associated factors predisposing to the development of ASB, to identify the causative pathogens and their antibiotic susceptibility profile. Methods: It is a hospital based observational study involving 100 pregnant women with no symptoms of UTI at various gestational age. ASB was identified by urine culture and sensitivity studies. Results: 15% of the participants were found to have ASB. There was no significant difference in the prevalence of ASB based on age groups, number of pregnancies, gestational age or presence of anemia. Gram negative organisms were the cause of ASB in two thirds of cases. Escherichia coli is the most common isolate followed by Staphylococci. Gentamycin, cefotaxime and nitrofurantoin were the most efficacious of the antibiotics studied. Conclusion: ASB is quite prevalent in the pregnant population. E.coli continues to be the leading cause of ASB over the decades but Gram positive organisms are increasingly being recognized as pathogens responsible for ASB during pregnancy. Antibiotic susceptibility pattern differ widely among different study populations and antibiotic stewardship is a must for appropriate treatment and to prevent resistance development.

Isolation of bacteria from pus samples and antibiotic sensitivity pattern in a tertiary care hospital, Lucknow

Dayavanti Kumari, Neeti Mishra, Khyati Tiwari

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 7, Pages 8492-8497

Introduction: In hospitals pyogenic infection is one of the major cause of morbidity. Increasing multidrug resistant strains has made treatment of such infection difficult. For correct antibiotic use, every pus samples should undergo culture and sensitivity.
Aim:Toisolatebacteriafrom pus samples and antibiotic sensitivity pattern in various pus isolates.
i)                    To study the distribution pattern and prevelance of bacteria causing pyogenic infection.
ii)                  TostudyAntibioticSensitivity Pattern of organism isolated from pus.
Methods: The study was conducted in the Department of Microbiology, T.S. Misra Medical College & Hospital, Lucknow, for a duration of 6 months. 256 pus samples from various wards with suspected pyogenic infection were studied. Bacteria were isolated, identified and antibiotic profile was determined from pus samples using standard protocol.
Results: In our study, out of 256 pus samples studied, 159 (62.10%) samples were positive for growth. Gram positive bacteria outnumbered gram negative isolates. Commonest isolate was Staphylococcus aureus, followed by Coagulase Negative Staphylococcus and Pseudomonas aeruginosa. All Staphylococcus aureus were sensitive to Vancomycin and Linezolid. All Pseudomonas aeruginosa were sensitive to Colistin. In Enterobacteriaceae group most effective antibiotic were Colistin and Tigecyclin.
Conclusion: It is important for a clinician to send all the pus samples for microbiological analysis and their antibiogram before putting cases on antibiotic so that emergence of drug resistance can be minimized.

To Assess Genotyphic Methods in the identification of ESBL producing Uropathogenic Escherichia coli

Kanaparthi Anil Kumar, Dr. Madhurendra Rajput

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 6, Pages 435-444

Urinary tract infections (UTIs) are the most common bacterial infections affecting approximately 11% of adult women each year globally, with approximately 60% of women experiencing UTI during their lifetime. Extended-spectrum β-lactamases (ESBLs) are extremely broad spectrum β-lactamase enzymes, which can be produced by Gram-negative bacteria. They are mainly found in a family of Enterobacteriaceae. ESBLs are produced by the mutation of the TEM-1, TEM-2, and SHV-1 β-lactamases.

Antibacterial effect of Swertia chirata against multi-drug resistant strains S. aureus and E. coli: in vivo and in vitro study

Bindu Sati, Sanjay Bhatt .

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 1106-1115

Misuse and overuse of antibiotics results in widespread multi-drug resistance. The major cause of increased mortality and morbidity rates is Multi-drug resistance to antibiotics and it becomes a global heath challenges now a days, therefore it becomes a necessity and need for researchers to seek for alternative and natural sources of antimicrobials. In the present study an experimental trial (In-vitro and In-vivo) was undertaken to examine the antibacterial activity of extracts of Swertia chirata leaf against multi drug resistant (MDR) E.coli and S.aureus. Broth dilution test for MIC and Disc diffusion method for antimicrobial susceptibility test were applied to investigate Antibacterial activity of plant methanolic extracts. Twenty five albino mice weighted between 180-200g were used for bacterial inhibitory activity (In- Vivo). Plant extract showed broad spectrum of antibacterial activity against S.aureus in comparison to E.coli and showed MIC values of 20µg/ml. Swertia chirata plant extract contains lesser inhibition zone against E. coli (8.7±0.80) and showed highest zone of inhibition against S.aureus (16.2±0.60). It can be concluded that Swertia chirata plant extract was significantly better (P<0.05) against S.aureus than E. coli. The number of bacteria in treated groups was very low, These findings have cleared demonstrated that the clearance of bacteria from the blood of infected mice by sub-MIC of plant extract was significant. the findings of present study provide a suitable evidences for the use of a drug composed of plant extract as a new generation of drugs to attack the antibiotic resistance of bacteria.

ClinicalStudy ofBacteriologicalPatterns andAntibiotic Sensitivity in Secondary Peritonitis

Naveen Banoth, Venkatesh A

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 2461-2472

Background:Intra-abdominal infections have been treated with various antibiotic regimens. These infections have been treated with single-agent and combination treatments. But no one therapy has been proven superior. The study's main goal is to examine the bacterial patterns in peritoneal fluid from surgical peritonitis patients and assess their antibiotic sensitivity and resistance.
Materials and Methods: It is a cross sectional observation research conducted in January 2020 to May 2021 which was conducted in JSS Hospital Mysuru. Analyzed data was in rates, proportions, and percentages. The sample includes 100 cases of secondary peritonitis caused by hollow viscus perforation, where preoperative peritoneal fluid samples were analysed for bacterial culture and sensitivity.
Results: Out of 100 samples, 50 had bacterial growth. The most common bacteria were E. coli. These were Acinobacter (6%), Candida (4%), Citrobacter (1%), Klebsiella (11%) and Serratia (2%). (4 percent). 77.5 percent of E. coli were sensitive to Ceftriaxone, 75% to Piperacillin-tazobactam, and 99.1 percent to Meropenem. In 40% of cases, E.coli was multidrug resistant. Most Klebsiellapneumoniae were responsive to ceftriaxone, piperacillin-tazobactam, and meropenem. Ciprofloxacin, Ceftriaxone, and Meropenem were all sensitive to Proteus mirabilis. 25.2 percent of cases were multidrug resistant bacteria.
Conclusion: The results of this investigation identify the organisms usually isolated from peritoneal fluid, their susceptibility and resistance to broad spectrum antibiotics. It shows the common gramme negative isolates and the current antibiotic resistance concern in these individuals. The most common microorganism found in gastrointestinal perforations is Escherichia coli. In most cases, numerous gramme negative bacilli develop polymicrobially. Antibiotic-resistant microorganisms, notably multidrug resistant Escherichia coli, are increasing in number. Third generation cephalosporins are becoming more resistant. In multidrug resistant organisms, Meropenem and Tigecycline seem to be the best options.

Escherichia coli Isolated from Horses and Study the Effect of the Peganum harmaline Extract In Vitro and In Vivo and Antibiofilm Effect In Vitro

Aseel Mohammed Hamzah

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 202-207

Out of a hundred horses, fecal samples Escherichia coli was isolated from 37 samples of
different ages, The isolated samples had been used to examine the effect of peganum
harmaline on isolated E.coli in vitro and in vivo.
The goal of this research was to determine the antimicrobial activity of peganum
harmaline extract by means of (ethanol: methanol 1:1) in opposition to Escherichia coli at
various 40,20, 10, 5, 2,5, 1,25 and 0,625 mg/ml concentrations in each plastic and glass
tube. The values of MIC (minimum inhibitory concentration) and MBC (minimum
bactericidal concentration) for the extract against E.coli were equal to (0.625 mg/ml) for
MIC and (10 mg/ml) for MBC on bacteria which cultured on glass tube while the MIC
value was 40 mg/ml and MBC was10 mg/ml on plastic tube. The effect of Peganum
harmaline on the formation of E.coli biofilm was investigated and the biofilm inhibitory
concentrations were 40-6.25mg / ml in vitro. In vivo, a group of laboratory mice used, the
LD50 of peganum harmaline extract was tested orally by up and down method and found
to be 1030 mg/kg body weight, the extract used as 103 mg/kg bodyweight treatment after
causing E.coli infection at 1×108 CFU/ml in laboratory mice the treated persist for two
weeks in group one and three weeks in the second group as well as three week treated in
the third group from the second days after infection while the control group left without
treated and the mice sacrificed after second, third and fifth days of infection. The result
shows histopathological changes in all treated groups, especially in the third group and
that refers to the antibacterial effect of the peganum harmaline ethanolic extract when
compared to the infected control group.