Online ISSN: 2515-8260

Keywords : Vitamin B12


EFFECT OF GLYCEMIC CONTROL ON VITAMIN B12 METABOLISM IN TYPE 2 DIABETES MELLITUS PATIENTS

Dhiraj Mahaseth, Vikas Kumar Gupta, Bijay Kumar Mahaseth, Ashish Kumar Sharma .

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 4981-4987

Background: Diabetes mellitus is a metabolic disorder due to absolute or relative insulin deficiency. Polyuria in type 2 DM leads to loss of important water soluble nutrients in urine. In view of widespread deficiency of vitamin B12 in our country and increasing prevalence of diabetes mellitus type 2, it is considered worthwhile to assess vitamin B12 status of patients with diabetes mellitus type 2.
Material and methods: Present study comprises of 60 subjects of both sexes whom 30 are healthy controls and 30 are clinically confirmed cases of diabetes mellitus, age group ranges from 40-70 yrs. Serum separated from plain vial after centrifugation was used for estimation of serum vitamin B12 by ELISA, Plasma glucose estimation by GOD-POD Method, End Point, Glycated hemoglobin by Boronate Affinity chromatography (NycoCard).
Result: In our study it was found that serum vitamin B12 (218.24 ± 68.31 pg/ml) was significantly lower in type2 diabetes mellitus patients compared to serum vitamin B12 (254.20 ± 64.89 pg/ml) of controls. Serum vitamin B12 level (190.20 ± 19.44 pg/ml) is significantly lower in diabetes mellitus patients who were suffering from the disease for more than 3.5 years as compared to serum vitamin B12 level (257.18 ± 88.74 pg/ml) of diabetes mellitus patients who were suffering from the disease for less than 3.5 years.
Conclusion: Thus, it can be concluded that there is significant vitamin B12 deficiency in type 2 diabetes mellitus patients.

A Hospital Based Prospective Study to Estimate the Prevalence of Vitamin B12 Deficiency in Vegetarian Outpatients Between 18-60 Years of Age Presenting at a Tertiary Care Centre

Devendra Dadhich, Darshan Kumar Bhargava, Mukesh Verma, Rajesh Meena, Ummed Singh

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 10954-10962

Background: Vegetarianism has been well known and commonly found in India since
ancient times. Animal products provide the only dietary source of vitamin B12. Vegetarianism
is a well-known risk factor for vitamin B12 deficiency. The aim of this study to determine the
prevalence of vitamin B12 deficiency among vegetarian outpatients, between the age groups
of 18 and 60 years, visiting the internal medicine outpatient department of a tertiary level
teaching hospital.
Materials& Methods: A hospital based prospective study in department of medicine at
government S.K. Medical College, Sikar, Rajasthan, India during one year period. A
prevalence study for any factor is best done in the community – in a lot of situations that
would reduce the bias of a hospital study. This being a hospital study has its own limitations.
However as far as possible we wanted to choose a population in our hospital survey that
would most closely reflect the community or the general population at largeVegetarian
patients were defined as patients that had been consuming a diet devoid of any form of meat,
at least for three completed years prior to the date of recruitment. At the point of contact with
the subject in the outpatient department he or she also underwent certain biochemical and
haematological tests that included serum vitamin B12 and folate levels, and basic
haemograms.
Results: In our study population, 61.25% had levels below 200, 22.5% had levels
between200and300and16.25%hadlevelsabove300 pmol/L. The mean vitamin B12 levels in
the three groupsrespectively were 144.8, 269.2 and 233.4. There was no statistically
significant differencebetweenthe groupsbyANOVA. At various laboratory parameters among
the three varieties of dietconsumers – however the numbers were too small for any statistical
analysis betweenthem. Mean corpuscular values of less than or equal to 100fl were
European Journal of Molecular & Clinical Medicine
ISSN 2515-8260 Volume 9, Issue 3, Winter 2022
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categorised asnormocytic category and anything higher than that was considered as
qualifying formacrocytosis. A folate level cut-off at 9.5 was also taken to look for prediction
ofvitaminB12 deficiency.
Conclusion: The vegetarian diet can be sustainable at all stages of life and in all
physiological conditions, including infancy, pregnancy, lactation, senescence and sports.
However, underestimating the correct supplementation of cobalamin (Cbl) can nullify these
benefits. It is also necessary that the diet be balanced and nutritionally adequate to reduce the
risks of other deficiencies which could indirectly affect the absorption of Cbl.