Online ISSN: 2515-8260

Keywords : acetonitrile, Artemether, isocratic, Lumefantrine


SIMULTANEOUS ESTIMATION OF ARTEMETHER AND LUMEFANTRINE IN COMBINATION DRUG PRODUCTS BY RP-HPLC METHOD

Meka.Srinivasa rao; Dr.K. Rambabu

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 11, Pages 8347-8367

This abstract illustrate an precise, isocratic RP-HPLC strategy have been
created by the creator for the concurrent estimation of artemether and lumefantrine in
unadulterated and advertised details by utilizing Inertsil C18 column (250×4.6 mm, 5μ)
improved mobile phase containing phosphate buffer(pH 4.5) and acetonitrile in the extent
of 40:60 %v/v and Discovery wavelength at 218nm. The retention times were 2.207min
and 3.733min for artemether and lumefantrine respectively. The linearity range was
found to be 10-30μg/ml for artemether and 20-60μg/ml for lumefantrine individually. The
developed method was Validated for specificity, system suitability, precision, linearity,
accuracy, Limit of Detection, Limit of Quantification, robustness, and Stability and the
examine results acquired for all the approval parameters by this proposed strategy were in
reasonable concurrence with ICH standards. Thus, the developed RP-HPLC method
represents another good alternative for the already existing HPLC methods especially
those using certain types of detectors which are not present in most of the laboratories