Online ISSN: 2515-8260

Keywords : zebrafish embryo development


Tuan Van Ngo; Le Thi Lam .; Cang Ngoc Ly; Tram Thi Bich Tran; Huy Nghia Quang Hoang; Chi Nguyen Quynh Ho; Mai Thi Phuong Nguyen; Tram Le Ngoc Vo; Thuan Thi Tran; Nga Thi Nguyen; Long Thanh Le; Thao Thi Phuong Nguyen

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 2681-2687

This study aimed to assess the effects of lead treatment on zebrafish embryo development. Embryos were treated with lead in various concentration at 0.1 μg/l, 1 μg/l, 10 μg/l, 20 μg/l, 100 μg/l. The results demonstrated that there was no difference in ratio of live embryo between control group and lead treatment group for 24 h. However, the live ratio of embryo was reduced in the high concentration of lead treatment from 72 h to 168 h. Lead treatment induced an increase heart rate in zebrafish embryos for 48 h and 72 h. In addition, the down-regulation of GADD45A (Growth arrest and DNA damage-inducible protein 45 alpha), GADD45G (growth arrest and DNA-damage-inducible 45 gamma), SOD1 (superoxide dismutase 1) and SOD2 (superoxide dismutase 2) was observed in zebrafish embryos for 168 h of lead treatment.