Online ISSN: 2515-8260

Keywords : streptococcus mutans

Antimicrobial effect of mushroom and ozone gas individually or combined

Shaimaa Ahmed Alrafee, Maha Ahmed Niazy, Khaled Hussein Metwaly .

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 3083-3089

Purpose: The purpose of this in-vitro study was to evaluate the effect of mushroom extract and ozone gas on streptococcus mutans.
Materials and methods: Suspensions of streptococcusmutans (NCTC 10449) in salt buffer were used to check the antimicrobial activity of aqueous extracts of mushroom and ozone. The test samples were divided as follow:Group I: Aqueous extract of mushroom, Group II: ozone gas, Group III: Aqueous extract of mushroom+ ozone gas. After exposure to different treatment, aliquots of the samples were spread on agar plate which was incubated at 37 C for 2 days. The number of bacterial colonies (CFU) on the plates was counted.
Results: There was a significantdifference between different groups (f=31.69, p<0.001). The highest value of bacterial count was recordedfor ozone therapy (8.97±0.18), followed by mushroom extract (8.69±0.45), while the lowest value of bacterial count was recorded for mushroom extract + ozone therapy (3.36±0.12).
Conclusions: mushroom extract + ozone gas show synergistic effect on streptococcus mutans.

Comparison of the Effects of the Leaf Extract and Gum of Pistacia atlantica and Chlorhexidine on the Growth Initiation of S. mutans: An in vitro Study

Saeedeh Mokhtari; Ataollah Ahrari; Zahra Hosseini

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 2688-2697

Background: Dental caries is one of the most prevalent infectious diseases, the main etiologic factor of which is Streptococcus mutans. The present study aimed to evaluate the effect of the leaf extract and gum of Pistacia atlantica on the growth inhibition of S.mutans in comparison to chlorhexidine (CHX).
Methods: In the present in vitro study, the antibacterial effects of the extract and gum of this plant were evaluated in comparison to that of CHX. The microbial sensitivity was evaluated by determining the diameter of the growth inhibition zone. In addition, the turbidity test was used to determine the minimum inhibitory concentration (MIC) of the materials against S.mutans.
Results: No growth inhibition zones for S.mutans were detected around the materials derived from P. atlantica, while a 24-mm growth inhibition halo formed around CHX. The MIC values for CHX and the leaf extract and gum of P. atlantica were 1.256, 1.8 and 1, respectively.
Conclusion: In general, the present study showed that the antibacterial effect of CHX on S. mutans was higher than those of P. atlantica derivatives. In addition, the P. atlantica leaf extract exhibited higher antibacterial activity compared to its gum, which is used as chewing gum in some regions