Online ISSN: 2515-8260

Keywords : Antimicrobial


Potential Synergistic Antimicrobial Activity of Extract from Peelings of Common Local Fruits: An In-Vitro Analysis

Laarni M. Villanueva- Magsombol; Carina R. Magbojos- Magtibay

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 4175-4185

The objective of this study is to investigate the potential synergistic antimicrobial activity of methanolic extract from peelings of Philippines’ common local fruits, namely, orange, mango, and papaya. Peelings from these local fruits were properly washed, dried and powdered. Extracts from the peelings were obtained using methanol as the solvent, concentrated in a rotary evaporator set and were finally reconstituted in 20% dimethylsulphoxide (DMSO).The preliminary determination of antimicrobial effect of the individual fruit peelings was tested using plate count method. A two-fold dilution of the individual formulated extracts were done and combined, after which the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using Staphylococcus aureus and Escherichia coli isolates were determined. MIC results of the mango extract showed best antimicrobial effect, which worked at a minimum concentration of 6.25mg/ml on both isolates. This was confirmed by the MBC, which showed the absence of growth of viable microorganisms after 24-hour incubation on nutrient agar. Orange and papaya were found effective against S. aureus at a minimum concentration of 50mg/mL. However, these extracts did not demonstrate individual antibacterial activity against E. coli. The combination of mango and papaya was the most effective among the three sets of extracts used in the synergistic antibacterial activity. It was found to be effective against E. coli at a minimum concentration of 6.25mg/mL and against S. aureus at 25-100mg/mL. Other combination of extracts such as orange-mango and orange-papaya exhibited synergistic antibacterial activity at higher concentrations. Moreover, orange and mango combination had a better synergistic activity against E. coli than S.aureus while orange and papaya if combined has greater synergistic activity against S. aureus than E. coli. From this study, it can be concluded that the combination of the extracts of mango and papaya has a more potential synergistic antimicrobial activity against S. aureus and E. coli.

Study Of Specific Activity Of The Hemostat Liquid Extract

Feruza Saydalieva; Nodira Yunuskhodjaeva; Ziyoda Fayzieva; Manzura Mullajonova; Rokhila Umurzakova; Shakhzoda Ramazonova

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 3362-3374

Our studies have suggested that the mechanism of action of the sum of flavonoids (liquid extract "Hemostat") from pylori, pylori, nettle and nettle in a ratio of 1: 1: 1 on the blood coagulation process is associated with its effect on the number, adhesion and platelet aggregation, as well as the retractive ability of platelets. It was revealed that the liquid extract "Hemostat" has a pronounced hemostatic effect and in this respect it is noticeably superior to the known preparation of the liquid extract of the shepherd’s bag.

Antimicrobial applications of green synthesized zinc oxide nanoparticles

R. Devanathan; B. Vivekanandhan; S. Rengadurai; R. Lakshmipathy; G.L. Balajia

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 4, Pages 1314-1321

Zinc oxide (ZnO) nanoparticles were fabricated by green process in presence of aqueous extract of Watermelon rind. High Performance Liquid Chromatography technique was used to assess the biomolecule content of the aqueous extract prepared from watermelon rind. Citrulline an essential amino acid present in watermelon rind was found to act as capping agent for fabrication of ZnO nanoparticles. The shapes of produced ZnO nanoparticles were found to be anisotropic with few nano rods which were confirmed by TEM analysis. The prepared ZnO nanoparticles were used as antimicrobial agent against pathogens. Higher activity was found against S. paratyphi B strains. Further, the ZnO nanoparticles were assessed for its heavy metal removal capacity from aqueous solution. The results suggest that watermelon rind can be a potential precursor for fabrication of ZnO nanoparticles with versatile applications.

Synthesis, Characterization And Biological Evaluation Of Schiff Base Of 5-Chloro Salicylaldehyde Hydrazine N-Methyl Carbo- Thioamide And Its Metal Complexes: Cytotoxicity, Dna Interaction & Incision, Antibacterial And Antifungal Studies

Pilli Jyothi; C. Gyana Kumari

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 8, Pages 4339-4355

A Schiff base ligand of N-methyl Thiosemicarbazide and its complexes with Co metal ion
of binary [Co(L)2] (Complex-A), and ternary, formulae [Co(L)(L1)(H2O)] (Complex-B),
[Co(L)(L2)(H2O)] (Complex-C) where L is Schiff base ligand, L1 is Bi-pyridine and L2 is
oxalic acid have been synthesized and the compounds had been characterized based on the
several spectroscopic results. The interaction of these compounds with DNA was
investigated by electronic absorption spectroscopy and fluorescence spectroscopy. Our
experiments indicated that these complexes could strongly bind to CT- DNA via
intercalation mode with Kb values of 1.2 x 104 M-1 to 5.7 x 104 M-1 and the nucleotide
incision against super coiled pBR322 DNA had been investigated by both photolytic and
oxidative techniques to reveal the efficient manner of cleavage by the metal complexes.
Furthermore, these complexes exhibited significant in vitro Cytotoxicity against HeLa &
MCF7 cell lines showing IC50 values around 46.03 to 62.05 μM. Finally, an antimicrobial
assay of these complexes over one gram-positive bacterium of Bacillus Subtilis and one
gram-negative bacterium of Escherichia Coli and also two fungal species had given a good
result.

Drug Induced Oral Pigmentation : A Review

Dr Vrushali B. Zamare; Dr Rahul R. Bhowate; Dr Vidya K. Lohe; Dr. Mrunal Meshram

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 1809-1815

Pigmented lesions of the oral mucosa are encountered on a routine basis in clinical practice. Oral health-care providers must assess several parameters associated with pigmented lesions. Oral mucosal color changes can be significant and gives diagnostic value of local or systemic disease though it requires clinical experience and thorough evaluation. Color changes in the oral mucosa can be attributed to the deposition of either endogenous or exogenous pigments as a result of various mucosal diseases. The various pigmentations can be in the form of blue/purple vascular lesions, brown melanotic lesions, brown heme‑associated lesions, gray/black pigmentations. Therefore, watchful examination of all the oral mucosa is frequently required. There are variable manifestation of oral pigmentation. The specific shade, extent, site, spreading of lesion gives diagnostic importance. Interactions between the dentist and the patient's medical specialist are very important to provide a definitive diagnosis as it increase the possibility to recognize these forms of lesions. Further and more work will be done to clarify the pathogenesis and to improve the diagnosis of the disease. The historical and current scientific history and the comprehensive research on the use of patients' medications have played a vital role. Such pigmentations can be caused by both systemic and topical drugs. Interactions between the dentist and the patient's medical specialist are very important to provide a definitive diagnosis as it increase the possibility to recognize these forms of lesions.

IN THE CONDITIONS OF COVID-19,SYNTHESIS AND ANALYSIS OF COORDINATION COMPOUNDS OF COPPER (II) CHLORIDE WITH MONOETHANOLAMINE AND P-NITROBENZOIC ACID

Khudoyberganov Oybek Ikromovich; Ibragimov Bakhtiyar Tulyaganovich; Ashurov Jamshid Mengnorovich; Ibragimov Aziz Bakhtiyarovich; Khasanov Shodlik Bekpulatovic; Azizjanov Khushnud Maksudovich

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 3147-3154

The article highlights the study of the synthesis of a complex
combination of p-nitrobenzoic acid with Cu2+from aromatic carbonic acid
derivatives. Influencing factors on the synthesis of complex compounds in the
room were studied. Specifically, the time dependence of the reaction, the effect
of temperature, the concentration dependence wasobserved in practice. The
synthesized complex was evaluated by means of IR-Fourier spectrometry,
elemental analysis, mass spectrometry, TG-DSK and X-ray structural analysis to
determine its chemical structure, as well as to justify that the new complex is
thermally and chemically steady.

ROLE OF COMMONLY USED ASIAN SPICES IN BOOSTING IMMUNITY AGAINST INFECTIOUS AGENTS

Kethan Umakanth; Geetha RV; Smiline Girija AS; Lakshmi T

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 452-458

Spices are normally used for adding flavour to the food. Especially Asians use a variety of spices or spice blends to make food aromatic, hot, savoury and sweet. Spices not only add aroma to the food but also come with notable nutritional values and give a lot of health benefits. The spices that are typically used in Asian cuisine are Cinnamon, garlic, cloves, cumin, basil, star anise, galangal, ginger, coriander, turmeric, cilantro, pepper, ajowain etc. The main aim is to try and understand the role of commonly used Asian spices on boosting immunity against infectious agents. A large number of plants are used to treat many diseases as they possess antimicrobial activity. However, Challenges include Extinction or disappearance of a few indigenous varieties, Low productivity, Insufficient quantities of quality species and Poor product quality. Many people do not know the importance of spices and its effect on the immune system. There is a need to understand the traditional systems, to help visualise the future better . there is a necessity to link the past and the present of medicine. This can help in giving new directions for a better understanding of different diseases etc. As this is a review various other related research articles are acquired using the Search engines such as PubMed, google scholar, google Cochrane databases and MESH. Their data is taken as the base for our study.