Online ISSN: 2515-8260

Keywords : liver


CORRECTION OF METABOLIC PROCESSES UNDER EXPOSURE TO THE PESTICIDE PARARGYTE WITH THE ADDITION OF A PLANT PREPARATION COMPLEX

Aziza Nishanova; OishaAlaviya .; AdilovUtkir .; KhamrakulovaMukaddaskhon .; SadikovAskar .; ImanovFurkat .

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2769-2784

Introductory information/relevance (Background): Widespread
prevalence of toxic hepatitis from chemical factors, the growth of chronic forms
of the disease, leading to a decrease in working capacity of the working
population, determine the multidimensionality of the problem and the relevance
of the development of new methods of prevention and treatment using a set of
herbal medicines.
Objective: Experimental study of the influence of the pesticide parargyte
on the functional state of hepatocytes and correction of metabolic processes with
the use of decoction from the complex of plant preparations.
Methods: Experimental studies were carried out on 146 white male rats
weighing 190-210. The mechanism of biological action of hepatotropic factors
was studied in acute and chronic experiments and correction of metabolic
processes with the addition of plant antioxidants.
Results (Findings): Experimental studies revealed that under the
influence of the pesticide propargit was observed a disturbance of carbohydrate
energy metabolism in the liver in all study periods. Application of a complex of
plant preparations to poisoned animals normalized the carbohydrate energy
metabolism and improved the functional state of the liver.
Conclusion: Application of the complex of herbal medicines - nettle
leaves, black currant fruits, rose hips, mountain ash fruits, green tea with
pesticide poisoning has a hepatoprotective effect on liver metabolism.

Determination Of Residual Pesticide In The Liver Of Rats Poisoned With Indoxacarb Pesticide

Parpieva Mashkhura; Mirkhamidova Parida; Tuychieva Dilfuza

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 4497-4505

An aqueous solution of indoxacarb pesticide was sent to rats by the Peroz method at a dose of 1/10 LD50 once via zond and the amount of residual pesticides in the rat was determined at 5, 10, 20, 30, 40 days after poisoning. For the analysis of residual pesticide quantities, the HPLC MS (6420) Tripl Quad LC/MS (Agilent Ttchnologies, USA) device was used. As a method of ionization, APCI(Atmospheric pressure chemical ionization) method was used.

CLINICAL JUSTIFICATION FOR THE USE OF PHYTO TEA "ANTIOXIDANT" IN PATIENTS WITH TOXIC LIVER DAMAGE

KhamrakulovaMukaddaskhon .; AdilovUtkir .; SadikovAskar .; NarkhojaevaShahnoza .; SaginbekovKadirjan .

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2738-2746

Background: The broad development of the chemical industry, the use of
pesticides in agriculture has led to an increase in hepatitis. At the same time,
different substances in their structure can cause liver function failure.
Objective: The objective of this work was to assess the functional state of
the liver of animals poisoned with the pesticide Bagira, using in their diet dishes
made from local food and additional introduction of a complex of local herbal
medicines into the diet.
Methods: The study involved patients with toxic hepatitis who had
industrial contact with chemicals. The total number of patients was 80 males,
aged 35 to 78 years.
Results (Findings): Based on the results of our research, the antioxidant
phyto tea helps to eliminate cytolytic, cholestatic, inflammatory syndrome,
improve the protein-synthetic function of the liver, improve the functional
activity of the liver, remove toxins from the body, maintain the function of
organs and systems. It is a natural, safe, powerful antioxidant.
Conclusions: 1) Studies have shown that patients diagnosed with chronic
hepatitis have disorders of the functional state of the liver, as well as the
gastrointestinal and nervous systems.
2) Change of biochemical indicators indicates a violation of metabolic, proteinsynthetic,
biliary, antitoxic functions of the liver: increase of bilirubin fractions,
the activity of alanine transferase (ALT), AST, the level of alkaline phosphatase,
gamma-glutaminetranspeptidase (GGT) in serum.
3. The use of phyto tea "Antioxidant" in patients with toxic liver damage
contributed to the increase of liver functional activity.

Influence Of Karate On The Activity Of Enzymes Of The Anti-Oxidizing System Of Rat Liver Protection And Ways Of Their Correction

Parida Mirkhamidova; Dilfuza Tuychieva; Dilnoza Bobokhonova; Mashkhura Parpieva

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 3757-3765

As a result of research, it has been established that synthetic karate pyrethroid causes functional changes in antioxidant enzymes in microsomes and mitochondria. An important aspect of our study was the comparison of the taxation rate when animals were poisoned with a karate pesticide and when animals were protected from poisoning with an antioxidant of plant origin. The results obtained indicate that the plant antioxidant factor (PAF) has protective properties and accelerates the restoration of enzyme activity.

Effect Of Pesticides On The Content Of Cytochrome P-450 Of The Monooxygenase System And On The Ultrastructure Of Hepatocytes In Rat Embryos

Tuychieva Dilfuza; Mirkhamidova Parida; Parpieva Mashhura; Babakhanova Dilnoza; Alimova Rano

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 2473-2483

Our results show that the pesticides butylcaptax, dropp and karate are highly toxic pesticides, when exposed to these pesticides, the activity of the enzyme of the P-450 monooxygenase system is disrupted. Also, the research results showed that when poisoning with the above pesticides, ultrastructural, and therefore functional changes in the subcellular components of embryonic hepatocytes occur. When using RAF (plant antioxidant factor), enzyme activity and other indicators are significantly restored, which indicates that RAF has antioxidant properties.

PECULIARITIES OF THE CHEMICAL AND PHYSICAL FACTORS INFLUENCE OF THE PRODUCTION ENVIRONMENT ON THE CARBOHYDRATE AND ENERGY METABOLISM OF THE EXPERIMENTAL ANIMALS’ LIVER

MukaddaskhonKhamrakulova .; SabirovaGulchehra .; SadikovAskar .; SadikovKhumoyun .; NavruzovErnazar .

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2810-2822

The energy supply of liver functions is carried out mainly by aerobic mitochondrial
processes. We have studied some aspects of carbohydrate energy metabolism in
mitochondria of the liver at the chronic influence of dichloroethane complex of factors,
noise and vibration on experimental animals’ organism. The research aims to study some
parameters of carbohydrate energy metabolism in mitochondria of the liver under the
chronic influence of dichloroethane, noise and vibration. The researchers carried out
experiments on 98 purebred white male rats weighing 160-180 g. Dichloroethane in the
form of 10% oily solution was injected into the animal’s stomach in a dose of 1/20 LD50
(255 mg/kg), in conditions of noise (95-110dBa) and vibration (2-16dBa) during 15 and 90
days. After the experiments end in 15, 30, 60 and 90 days after exposure cessation to
chemical and physical factors, the rats were decapitated and determined some indicators of
carbohydrate and energy metabolism in the liver and blood of experimental animals.
Presented results of experimental studies show that at repeated exposure to dichloroethane,
noise and vibration there was observed an increase in the intensity of anaerobic glycolysis
in blood, which is confirmed by the accumulation of lactic acid and reduction of glycogen.
Similar changes were observed in the reduction of activity of alkaline phosphatase in the
liver. Inhibition of enzyme activity (dehydrogenase) in all studied biospheres can be
explained by the influence of dichloroethane, noise and vibration on enzyme functional
activity. Inhibition of enzymes GDG, MDG, SDG and alkaline phosphatase in the liver is
observed at multiple effects of dichloroethane, noise and vibration. As a result of the
reduction of redox enzymes, under-oxidized products of anaerobic glycolysis of lactic,
pyruvic acid are accumulated and the level of glycogen is reduced. Prolonged exposure to
various chemical and physical factors in concentrations causing external effects in the
experimental animals’ organism is manifested by hidden changes in several physiological
reactions, biochemical indicators of the individual organs function and systems.

A Mini Review On Liver Injuries Among Humans Due To Drugs And Consumption Of Alcohol

Sumaira Yousuf; Mahendra P. Singh

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 4268-4276

Liver is the most important vital organ that carries out metabolism of body e.g. formation of cholesterol and triglycerides, synthesis of glycogen, formation of blood clotting proteins and bile formation. Hepatic damage and subsequent liver failure due to both unintentional and intentional acetaminophen overdose has afflicted patients for decades and includes the metabolic cornerstone pathways that exist within hepatocytes in the microsomes. The side effects of drugs on the hepatocytes have been overlooked for years. Drug induced liver injury is the most adverse effect observed in clinical practices. It accounts for around half of acute liver disorder. Anti tuberculosis drugs and acetaminophen overdose are the main issues that result in death from curable diseases. Metabolism of isoniazid and acetaminophen (APAP) results in the formation of toxic metabolites that results in the oxidative stress and glutathione (GSH) store depletion leads to hepatocellular necrosis and steatosis. Alcohol consumption is also the most common cause of liver cirrhosis. Liver peroxidation is considered to be the major mechanism of alcohol-induced hepatotoxicity. To overcome this oxidative stress, there is need of antioxidants that can balance the redox homeostasis of liver by lowering the APAP overdose effect. Consumption of natural antioxidants derived as secondary metabolites from plants in the form of polyphenols and flavonoids prevent oxidative stress. Medicinal plants are best remedies used for hepatotoxicity caused due to oxidative stress. They are cost friendly, easily available and have no mutagenic effect. Herbal medicines form important part of traditional healthcare system of India.

Liver Micro-Circulator Back After Its Partial Resection For 15 Day Of Life

Askarov T. A.; Olimkhujayev F. Kh.; Akhmedov M. D.; Fayziev Y. N; Ashurmetov A. M.

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 8, Pages 4099-4106

In an experiment on 180 white mongrel male rats, 31-34% (left lateral lobe)
of the liver was resected on the 15th day of life. We found that after resection of the liver,
constantly occurring liver complexes were detected, which consist of 3 types of lobules. The
initial link of blood outflow from the hepatic lobes is the initial hepatic venules, which are
formed from the fusion of sinusoids in the subcapsular zone of the liver. After resection,
the main process is the formation of new structural and functional units – liver lobes in the
subcapsular area of the organ.