Online ISSN: 2515-8260

Keywords : liver disease


Study of Coagulation Profile in Liver Disease Patients at A Tertiary Care Hospital

Almas Sahir Chopdar; Sneha Suryaji Sangrame; Savita Sanjay Patil; Leena Ashok Nakate

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 5, Pages 595-603

Background: The study of coagulation can be traced back to about 400 BC and to the father of Medicine, Hippocrates. The liver is the cornerstone of the coagulation system. The liver is the site of synthesis of fibrinogen and factors Ⅱ, Ⅴ, Ⅶ, Ⅸ, Ⅹ, Ⅺ and Ⅻ. Von Willebrand factor (VWF) is synthetized by the endothelium. When both cellular and plasmatic coagulation are defective, represents a hallmark of advanced liver disease. There is substantial overlap in the hemostatic abnormalities observed in the patients with acute infectious or toxic hepatitis, chronic hepatitis, and cirrhosis, the severity of hepatocellular dysfunction is typically more informative than the etiology.
Aim & Objective:
1. To determine the coagulative abnormalities among liver disease patients admitted in medicine wards.
2. To study the association of coagulation abnormalities with the extent or severity of liver disease.
Methods: Study design: A Cross sectional study. Study setting: Department of pathology. Study duration: June 2019 to June 2020.
Study population: All patients of acute and chronic liver disease admitted to medicine wards in tertiary care hospital such cases included in the study.
Sample size: 200
Results: most common age group was between 31-40yrs of age, i.e., 32% of total patients. 174 (87%) were male population and 26 (13%) were female population.78(39%) cases of cirrhosis, 46(23%) cases of hepatitis, 44(22%) cases of Alcoholic liver disease, and 32(16%) cases of other liver diseases. patients showed coagulation abnormalities considering different parameters i.e., PT, APTT, Platelet count individually or in combinations, and 11(5.5%) patients showed normal test results. About 87% (174/200) had prolonged PT. Mean PT in present study was 28.33±22.29. P value was <0.05 i.e., 0.013 which was statistically significant
Conclusions: Cirrhosis was the most common pathology amongst the study subjects. The PT was most abnormal test among all tests performed. The platelet count and APTT were the least frequently abnormal test. Hence PT was most significant coagulation test among other tests. The proportion of raised PT was highest in alcoholic liver disease and lowest in case of other liver diseases. Coagulation abnormalities were significantly associated with the extent of liver diseases.

Lactate dehydrogenase as an indicator of liver tumors

Dr. Akshatha LN, Dr. Sandeep Kumar, Dr. Ramesh G3, Dr Rachana, Dr. Ramchandra C

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 1, Pages 826-830

Background: Lactate dehydrogenase (LDH, L-lactate, NAD+ oxidoreductase, EC1.1.1.27) is a
family of at least six NAD + -dependent isoenzymes (LD1-LD5 and LD6/LDX). It is recognized
as being one of the most common enzymes in nature. It belongs to the class of oxidoreductases
and it is characteristic for the final stage of anaerobic glycolysis. Aim of the study: To evaluate
the efficacy of lactate dehydrogenase as an indicator of liver tumors. Materials and methods:
The present study was performed in the Department of Biochemistry, Kidwai Institute of
Oncology, Bengaluru, Karnataka, India. For the study, a total of 100 patients were selected. The
age of the patients ranged from 18 to 40 years. Briefly, 20 μL of each patient serum was mixed
first with 1000 μL of buffer (pH 7.35) supplied by the manufacturing company and incubated for
1-5 min at 25 °C. A volume of 250 μL of substrate that composed of nicotinamide-adenine
dinucleotide (0.75 mmol/L) and sodium azide (0.095%) was added to the mixture. After mixing,
the absorbance at 340 nm was read by UV-visible spectrophotometer (CECiL, CE 1021,
England) after 1 min. CK levels were assayedaccording to Humazym M-test as described by the
instructions of the produced company. Results: It was observed that number of male patients
was 45 and number of female patients was 55. The mean age of the participating patients was
31.35 years. High LDH level are observed in large number in females (30 patients) than in males
(24 patients). Increased LDH levels in blood could not be indicator for liver diseases only, but it
may be resulted from damage in muscular tissues. 30 females and 24 males who possessed high
level of LDH were investigated for CK, as monitor for muscular injury. Additionally, 6 male
patients and 9 female patients showed increased levels of LDH and CK in their blood which
means they may sever from muscular damage. Conclusion: From the results of the present study,
this can be concluded that LDH can be used as a good biomarker for diagnosis of liver,
muscular and even cancer diseases. There is little variable between males and females in the
elevated values of LDH. Patients with normal values of CK and high level of LDH suggested to
have unidentified cancer disease, except liver cancer, and for that other specific tests are
required.

Identification and Prediction of Liver Disease using Logistic Regression

Neeraj Varshney; Ashish Sharma

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 4, Pages 106-110

Identification of disease at a beginning stage is very essential for higher treatment. It’s a awfully complicated task for medical researchers to predict the illness within the early stages because of delicate symptoms. Typically the symptoms turn out to be evident once it's too late. to beat this issue, this project aims to boost disease designation victimization machine learning approaches. The most objective of this analysis is to use categorization techniques to spot the liver patients from healthy people. This project conjointly aims to match the categorization techniques supported their presentation factors. To serve the medical community for the designation of disease between patients, a graphical computer interface is urbanized victimization python (Node RED). The GUI will be promptly used by doctors and medical practitioners as a screening tool for the disease.