Online ISSN: 2515-8260

Keywords : bacteria


STUDY of MICROBIAL AIR CONTAMINATION IN BIOLOGICAL LABORATORIES/ COLLEGE of SCIENCE

Dunia K. Salim

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 9, Pages 123-129

Objective: A study on indoor air play important roles in human health by polluted indoor labrotory and causes some diseases such as infections skin, eyes, respiratory system , allergy and biotoxicity. Air is made up of enormous of microorganisms mainly bacteria. Their estimation is important as an indication of cleanliness of any particular environment. The presence both bacteria and fungi in indoor air is problem of health protection due to a long time period that workers and students stay indoors. Determination of level microbial pollution indoors is necessary to assess the health hazard for indoor air quality control. This study provides information on microbial contamination level of indoor air of biological labrotary was estimated. Material and Methods: Collected air samples from seven bio-laboratories and low, up corridors locations during April-June 2019. Air samples were taken three months : in the in the afternoon. Results: The higher of percentage of isolated airborne bacteria Staphylococcus aureus and Staphylococcus epidermis, it was 58.57% and 26.84% for all biological laboratories respectively at study location, while the lowest percentage were Klebsiella sp. and Protus sp., it was 11.98% and 4.29% for all biological laboratories, respectively. Conclusion: It is concluded from the current study that is advisable that estimate to check microorganisms loads in Laboratories and prevent their increase and spread

Oil Contaminated Soils And Their Biological Recultivation

Z.A. Jabbarov; B.T. Jobborov; Sh.A. Xalillayev; V.Kh. Sherimbetov

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 6, Pages 2797-2810

The following studies have shown the results of low, moderate, strong and very strong soil contamination as well as the change of soil morphological characteristics, the strains separation of oil-breaking bacteria and the development of recultivation technology. According to the results, the changes in soil morphological characteristics differ in contamination degree, and the morphological characteristics of very strong soil contamination (more 25 g/kg) have dramatically changed. The oil-breaking bacterial strains such as MFD-100 Pseudomonas stutzeri, MFD-200 Pseudomonas caryophyllis, MFD-5000 Bacillus subtilis are separated from oil-contaminated soils. Based on these strains, the three-stage recultivation technology has been developed, and as a result of the conducted recultivation, the soil cover is purified up to 81.8%. Soil purification rate is divided into the following efficiency indicators: 1 - soil purification rate 0-20%, 2 - soil purification rate 20-40%, 3 - soil purification rate 40-60%, 4 - soil purification rate 60-80% and 5 - soil purification rate 80-100%. After the soil recultivation, the productivity indicators such as nutrient and humus content as well as pH environment are improved.

In vitro study of antibacterial activity of Entada spiralis Ridl. crude extract on selected skin infection-causing bacteria

Raihanah Hasaniah Abdullah; Risyawati Mohamed Ismail

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 3828-3832

In Malaysia, Entada spiralis Ridl. from Leguminoceae family grows wildly and the scientific study of it has not been explored deeply. Entada spiralis Ridl. is a liana or woody climber plant which is locally known as "akarbeluru" or "Sintok". This study is performed to evaluate the antibacterial activity of Sintok extract from the stem bark of Entada spiralis against skin bacteria infections by the disc diffusion method. Two types of skin bacteria known as Staphylococcus aureus and Streptococcus pyogenes were selected and tested against the water extracted Sintok. Sintok concentration of 1600mg/ml gave the highest inhibition zone diameter against Streptococcus pyogene rather than Staphylococcus aureus with a diameter of 25.5 mm and 18. mm, respectively. This study indicates clear evidence supporting the traditional use of Entada spiralis in treating skin infections related to bacteria

Association of bacterial/viral infections withneutrophil-lymphocyte ratio, monocyte-lymphocyte ratio, and platelet-lymphocyte ratio in patients presenting with fever

Tri Yulia Rini; Satriawan Abadi; Sudirman Katu; Syakib Bakri; Haerani Rasyid; Hasyim Kasim; Andi Fachruddin; Risna Halim; Arifin Seweng

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 1500-1509

Background of the Study: Bacterial and Viral infections are often hard to be distinguished in daily clinical practice. Biological markers obtained from a routine examination play an important role to minimize time in providing diagnose and giving therapy. Recently, the use of Neutrophil-lymphocyte ratio (NLR), monocyte-lymphocyte ratio (MLR), and platelet-lymphocyte ratio (PLR) is greatly considered to differentiate types of infection found in the patients presenting with fever.
Method: This study uses prospective cohort study design and involves patients presenting with fever who are admitted to the ICU. The initial NLR, MLR, and PLR is examined and categorized into types of infection found. The ANOVA test and t-test are performed to find out the difference among study groups with the value of α = 0,05.
Result : This study involves 207 patients (92 male patients [44%]) presenting with fever with the average age of 45,6 ± 14,6 years old. The majority of cases (135 cases [66,5%]) in patients with fever results from bacterial infection; The study also finds 47 cases (34,8%) of typhoid fever and 30 cases (22,2%) of pulmonary tuberculosis. Dengue hemorrhagic fever (DHF) is the most commonly found viral infection with 52 cases (76,4%). The significant diagnose of bacterial infection shows higher value of NLR and MLR than that of viral infection (P < 0,001); Urinary tract infection has the highest value of NLR and MLR, amounting to 9,4 ± 3,6 and 0,23 ± 0,20, respectively. In general, the value of PLR is lower than that of viral infection (P < 0,001).
Conclusion: Neutrophil-lymphocyte ratio, MLR and PLR have benefit to predict diagnosis for the patients presenting with a fever. Bacterial infection is associated with the high value of NLR and MLR, and PLR generally has a lower value in viral infection cases.

ROLE OF PROBIOTICS IN PREVENTION OF OBESITY

N. Naveenaa; Jayalakshmi Somasundaram; Leslie Rani. S

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 3213-3223

Probiotics can be defined as live microorganisms that may beneficially affect the host upon ingestion by improving the balance of the microflora. It has various health benefits like alternation in weight, increasing the immunity, and also in digestion. Many of us would think that bacterial products aren’t helpful but in contrast they are enormously useful. The common species involved are Lactobacillus, Bifidobacterium. At present the alarming issue for everyone might be weight gain. The probiotic diet helps in modification of gut microbiota and it inhibits the absorption of dietary fat and increases fat excretion via faeces. Most of the attempts done to study the effects of probiotics with Lactobacillus strains on weight reduction was found to be positive. Probiotic administration on women over a period of three months had a significant reduction in weight compared to the pills. So probiotics are far better than artificially prepared pills. The aim of the review is to analyze whether a probiotic diet helps in reduction of weight and how far it could play the beneficial role in reduction of weight.