Online ISSN: 2515-8260

Keywords : HIV

In-Vitro Screening of Reverse Transcriptase Activity of Selected Indian Medicinal Plants against Human Immunodeficiency Virus Type-1

Priyadarshini K; Kulandhaivel M; Sankareswaran M; Anbalagan S

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 11, Pages 5168-5175

Human Immunodeficiency Virus type-1 (HIV-1) is that the explanation for Acquired Immune Deficiency Syndrome (AIDS), a serious human viral disease.The high cost of the HAART regimen has impeded its delivery to over 90% of the HIV/AIDS population within the world. The aim of the present study was to evaluate the in vitro anti-HIV activity of selected Indian medicinal plant extracts.Extracts were prepared from dried plant Vitex negundo, Evolvulusalsinoides and Datura metel leaf with Hexane, Methanol, Ethanol, Chloroform, and Petroleum ether. The phytochemical activities and HIV-1 RT inhibition activity (HIV-1 RT colorimetric ELISA kit –Roche Kit method) was carried with the all solvent extracts of Vitex negundo, Evolvulusalsinoides and Datura metelwas determined. The methanolic extracts of Vitex negundo have given the maximum and positive result with 74.76±1.36, which proves that Vitex negundo has a potential antiviral activity against HIV. Phytochemical analysis conducted on the plant extracts revealed the presence of constituents which are known to exhibit medicinal also as physiological activities.The predominant components present altogether the extracts of Vitex negundo are flavonoids, Terpenoids and steroids. Thus this study seems to justify the normal use of plant for the treatment of communicable disease of viral origin.

An insight among students of health sciences towards HIV/AIDS: A cross sectional study in coastal Karnataka

Ankitha Prem; Mahima Mishra; Suhan .; Smitha Nayak; Suman VB; Anupama N; Priyanka Arun Shirali; Rashmi Kaup Shiva

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 6, Pages 26-37

The objective of this paper is to assess the level of knowledge about HIV/AIDS among Interns of a Dental and Medical College. The data is collected through structured questionnaire by cross sectional study method with the sample size of 246. The model is tested on a context of Interns of dental and medical students for the first time. The present study has used a reflective measurement model. Due to the model complexity, the use of Partial Least Square-Structural Equation Modelling approach is found to be appropriate for the purpose of analysis of constructs and their corresponding data. The structural model has proved that the path coefficient value and empirical t values of exogenous latent variables on endogenous latent variable are above that threshold value in relationship between knowledge and trust as well as perception and trust except attitude and trust. The testing of hypothesis confirms the strong relationship between knowledge and trust as well as perception and trust. Based on the results of Importance Performance Matrix Analysis results corporate need focus on changing the perception of interns primarily. Hence,medical college must aim at increasing the performance of the target construct trust so their first priority should be to improve the performance aspects captured by the construct perception performance by improving its total effects as this construct has high chance to improve its total effect which in turn improves its performance.


Sneha Sree. S; Abilasha Ramasubramanian; Jayanth Kumar

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 600-615

A virus is composed of nucleic acid either DNA or RNA, surrounded by a protein coat.It requires a living cell to multiply. A viral infection can lead to a spectrum of symptoms from asymptomatic to severe disease.Worldwide,5% of health-care-related infections are being transmitted. Dentists are considered being a high risk group for cross-infection.Therefore it is important that dental students have adequate knowledge and awareness of viral infections.The aim of the study is to evaluate the knowledge, awareness and attitude about oral manifestations of viral infections among dental students.A self explanatory questionnaire comprising 20 questions was presented to 205 students. The questionnaire enquired about oral manifestations of different viral infections and their precautionary infection control measures .Out of 205 students 86.34% were males and 13.66% were females,189 of them knew that the most common oral manifestation in HIV patients is candidiasis.92.6%agreed that a dentist could get Herpetic paronychia.When asked if treatment of patients with viral infections required special clinics 68.1% responded yes.Our study showed that the dental undergraduate students had adequate knowledge on oral manifestations of viral diseases but lacking knowledge about infection control protocol for the various diseases. Hence students should improve their attitude towards them in their dental practice.