Online ISSN: 2515-8260

Keywords : blaSHV


Molecular Characterization of Extended Spectrum Beta-Lactamase Producing Bacteria Isolated from a River Surface Water

Fazlul MKK; Mohamad Farouk Abdullah; Nomani Kabir; Saeid Reza Doustjalali; Negar Shafiei Sabet; Srikumar C; Rashid MA; Jassim M. SaadounMohammed A. KadhimSalah M. S. Al-Atab; Nazmul MHM

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 1, Pages 1570-1580

The existence and prevalence of multi-drug resistant extended-spectrum βlactamase (ESBL)-producing bacteria in the river water is a major cause of numerous diseases worldwide. In this study, the molecular characterization of antimicrobial-resistant bacteria producing ESBL encoding genes was investigated for a better understanding of the risk factors and public health issues. The potential ESBL-producing bacterial species were detected using 16S ribose ribonucleic acid (rRNA) polymerase chain reaction (PCR). This served as a screening step to detect potential ESBLs encoding genes which were confirmed by phenotypes (DDST and E-test) and genotypes (PCR) assays with the presence of the bla genes; TEM, CTX-M, OXA-1 and SHV. Furthermore, all the confirmed bacterial isolates producing ESBL encoding genes were analysed for antibiotic susceptibility patterns against 10 different classes of antibiotics as a choice of therapy using antibiotic susceptibility testing (AST) by the disc diffusion method. The highest bacterial isolates were confirmed as Enterobacteriaceae (89.5%), predominantly Escherichia coli and Klebsiella pneumoniae. Among the 20 bacterial isolates, 12 (60 %) bacteria possessed one or more ESBLs encoding genes. Relatively high occurrence rates of β-lactamase genes; bla TEM 35%, bla SHV 20%, bla OXA-15% and bla CTX-M 10% were recorded. All the ESBLs encoding isolates showed high resistance to penicillin’s, third-generation cephalosporins, monobactams, cephamycins and carbapenems. High occurrences of ESBLs producing bacteria in the environment pose a threat to exposed communities. Therefore, early detection of MDR beta-lactamase mediated resistance genes are essential to avoid numerous diseases due to the dissemination of ESBLs producing strains.

Molecular Study And Antibiotic Susceptibility Patterns Of Some Extended Spectrum Beta-Lactamase Genes (ESBL) Of Klebsiella Pneumpniae In Urinary Tract Infections

Ashwak B. AL-Hashimy; Weam K. Al-Musawy

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 8, Pages 14-22

The present study included the collection of 121 samples from MSU for investigating the presence of K.pneumoniae in UTIs, the samples have been collected from Al-Shaheed Mohammed Baqir AL-Hakeem hospital and private laboratories in Baghdad province. The study was carried out through March 2019 to the beginning of June 2019. The samples were identified based on the morphological and microscopically characteristics of the colonies when they were culturing on number of culture media as well as biochemical tests, molecular identification were also used as a final diagnostic test for isolates that were positive as they belong to K.pneumoniae bacteria during previous tests based on the blaTEM, blaSHV and blaCTX-M genes which has specific sequences for K.pneumoniae bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance. The results of the final diagnosis showed that 38 isolates belong to target bacteria, The examination of the sensitivity of all bacterial isolates was done for selected 38 isolation towards the 16 antibiotics by a Vitek2 compact ASTN system and the isolates were resistant for a number of antibiotics used such as; Amikacin (5.26%), Imepenem (5.26%), Ertapenem (7.89%), Meropenem (10.52%), Gentamicin (21.05%), Ciprofloxacin (26.32%), Cefoxitin (39.47%), Trimethoprim/Sulfamethoxazole (50%), Ceftriaxone (52.63%), Fosfomycin (55.26%), Piperacillin/Tazobactam (57.89%), Nitrofurantoin (57.89%), Ceftazidime (65.79%), Cefuroxime (71.05%), Cefixime (73.68%) and Ampicillin (100%). The presence of Extended Spectrum Beta-Lactamase genes in 38 K.pneumoniae isolates were 65.8 % of the ESBL genotypes expressed blaSHV genes followed by 52.6 % blaTEM and 42.1 % for blaCTX-M.

Molecular Study And Antibiotic Susceptibility Patterns Of Some Extended Spectrum Beta-Lactamase Genes (ESBL) Of Klebsiella Pneumoniae From Urinary Tract Infections

Weam K. Al- Musawy; Ashwak B. AL- Hashimy

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 6, Pages 796-804

The present study included the collection of 121 samples from midstream urine for investigating the presence of K.pneumoniae in urinary tract infections, the samples were collected from Al-Shaheed Mohammed Baqir AL-Hakeem hospital and private laboratories in Baghdad province. The study was carried outthrough March 2019 to the beginning of June 2019. The samples were identified according the morphological and microscopically characteristics of the colonies after culturing on number of culture media as well as biochemical tests, molecular identification were also used as a final confirmed diagnostic test for isolates that were diagnosed as K.pneumoniae bacteria based on the blaTEM, blaSHV and blaCTX-Mgenes which has specific sequences for K.pneumoniae bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance. The results of the final diagnosis showed that 38 isolates belong to target bacteria, The examination of the sensitivity of all bacterial isolates was done for selected these isolates towards 16 antibiotics by aVitek2 compact ASTN system and the isolates were resistant for a number of antibiotics used such as; Amikacin (5.26%), Imepenem (5.26%), Ertapenem (7.89%), Meropenem (10.52%), Gentamicin (21.05%), Ciprofloxacin (26.32%), Cefoxitin (39.47%), Trimethoprim/Sulfamethoxazole (50%), Ceftriaxone (52.63%), Fosfomycin (55.26%), Piperacillin/Tazobactam (57.89%), Nitrofurantoin (57.89%), Ceftazidime (65.79%), Cefuroxime (71.05%), Cefixime (73.68%) and Ampicillin (100%). The presence of Extended Spectrum Beta-Lactamase genes in 38 K.pneumoniae isolates were 65.8 % of the ESBL genotypes expressed blaSHV genes followed by 52.6 % blaTEM and 42.1 % for blaCTX-M.