Online ISSN: 2515-8260

Keywords : Oral Squamous Cell Carcinoma (OSCC)

An Immunohistochemical assessment of Hypoxia Inducing Factor 1 alfa expression in Oral Epithelial Dysplasias And Oral Squamous Cell Carcinomas.

Dr.P.Ranga Raju, M.D.S. Dr. N. Govind raj Kumar, M.D.S., Dr. Nagaraj Anand, M.D.S. Dr. P. Ahalya M.D.S. .

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 4, Pages 2266-2291

HIF 1α is the intrinsic survival factor of dysplastic and tumour cells, to overcome the oxygen and nutrient deficits in the progression of OED and proliferation of OSCC. The entire spectrum of HIF 1α in OED and OSCC is not yet understood. The present study was done to evaluate the nature of expression of HIF 1α in OED and OSCC.
AIM:The study aims to evaluate the IHC expression of HIF 1α in OED and OSCC and also to investigate whether HIF 1α can be a predictive marker for malignant conversion of oral premalignant lesion.
OBJECTIVES:To analyse the IHC expression of HIF 1α quantitatively and qualitatively in OED and OSCC and to compare HIF 1α expression in OED and OSCC.
Materials and methods: Neutral buffered formalin fixed paraffin embedded blocks of 30 OED, 30 OSCC and 10 normal buccal mucosa cases that fall into WHO criteria were retrieved from the archives in the Department of Oral Pathology, Vishnu Dental College Bhimavaram. Clinical data was taken from the submitted biopsy forms. IHC staining of HIF1α was done according to the protocol. Quantitative, Qualitative and Semiquantitative assessment of the staining was done with the help of image J software and statistically analysed by SPSS software.
 Results:  Of the 30 stained HIF1α OED cases, 25 expressed HIF 1α positivity, of which 9, 7 and 9 were mild, moderate and severe OED respectively, accounting for 83.3% of staining.HIF 1α stained 30 OSCC cases, showed positivity in 22 cases, of which 7, 8 and 7 were Well, Moderately and Poorly Differentiated OSCC  respectively, accounting for 73.3% of staining.
Conclusion: HIF1α a major contributor to tumor progression, is different in its IHC expression during different stages of OED and OSCC. Further studies could predict clinical outcome and survival of patients.

Automated classification of Oral Squamous cell carcinoma stages detection using Deep Learning Techniques

Dr. Abinaya. R; Aditya. Y; Dr. Bala Brahmeswara Kadaru

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 4, Pages 1111-1119

Deep learning have earned major popularity in the today world by captured best results in medical analysis field. This research explained the stages of Oral squamous cell carcinoma using the convolution neural network model in deep Learning. Whenever the pathologist examine the photomicrograph image they faced a lot of difficulties to process and finding the stages of oral squamous cell carcinoma into poorly differentiated, medium differentiated and low differentiated. To avoid the difficulties of stages differentiation, the convolution neural network model has been implemented in this research. In the methodology part of Deep learning basically needs large number of data to perform good result so in this work image augmentation was performed to improve the better performance level of deep learning. Finally segmentation has been implemented and the segmented values are given to the convolution neural networks and it gives better accuracy of 85% when compared with all other deep learning techniques

―Comparison of resection performed by Peroral and Transoral route on surgical margins in patients with oral squamous cell carcinoma.‖

Dr Chirag Sunil Patil; Dr. Anendd Arroon Jadhav

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2070-2078

Abstract: Backround: Lip-split incision has been traditionally used to manage the primary
oral cavity tumour to ascertain clear surgical margins. Small tumour excision is routinely
carried out per orally; however, there is a paucity of data vis-à-vis with the oncological
safety of per oral approach in T2/T3 excision of oral squamous cell carcinoma (OSCC).
Objective of the study: We compared per oral access over the transoral access in terms of
assessing the tumour free surgical margins. Settings & Design: A prospective,
interventional, double-blind, randomized control study was conducted among 20
histopathologically proven participants with previously untreated OSCC scheduled for
curative intent. Subjects & Method: Participants were randomly allocated to the two
intervention used. The primary outcome variables evaluated were the adequacy of the
surgical margin. Additionally, the primary tumour resection time between two
interventions was assessed. Results: The primary tumour resection time will be
significantly extended in Peroral access & no significant difference will be seen pertaining
to the adequacy of a safe surgical margin between two intervention. Peroral access will be
a choice of surgical approaches that preserve function, minimize complications and
maximize cosmetic outcome. Conclusion: Surgical approaches that minimalize
complications, preserve function with the optimal cosmetic outcome should be utilized
whenever feasible. Peroral access may offer significant advantages like avoiding transoral
trauma, fastens the healing time & affords better & faster recovery.