European Journal of Molecular & Clinical Medicine

Background:Liver dysfunction in dengue varies from mild injury with elevation of transaminases to severe hepatocyte injury. Despite hepatocytes are not a major target, hepatic dysfunction is the recurrent feature. Predictive hepatic serum markers represent a solution for the dynamic care of serious dengue and predicting disease prognosis. In the present study we pursued to mold the mode of hepatic involvement in children with dengue and its association with seriousness of disease.
Materials and Methods: This was a cross sectional analytical study conducted at Department of Pediatrics, Govt Medical College &hospital during the period of 18 months from October 2019 April 2021 on 120 children under 18 years of age diagnosed with dengue positive and similar age and sex matched controls were included in this study. After obtaining informed consent, Patient demographics, presenting symptoms, clinical signs, laboratory parameters such as complete blood count, serum AST, serum ALT, ALP, total and direct bilirubin; serum albumin levels were collected. P-value <0.05 and CI 95% were considered significant in all correlation analyses between transaminases and platelet count.

It is the most common and widespread arboviral infection in the world today caused by
Dengue virus. Dengue viruses (DV) belong to the family Flaviviridae and there are four
serotypes of the virus referred to as DV-1, DV-2, DV-3 and DV-4.The course of the disease
has changed in the recent years from milder form to severe form like dengue hemorrhagic
fever & severe dengue fever and with increasing outbreaks frequency.Data was collected by
face to face interview from the parents of the children admitted in pediatric emergency ward
with diagnosis of dengue fever and severe dengue fever in Department of pediatrics. The
presence of bleeding manifestations like melena, epistaxis, hematemesis, petechiae,
hematurias more in DF with warning signs(37.5%) and Severe dengue fever(55.6%). There is
significant association between bleeding manifestations and severity of dengue
fever(p<0.001). Thrombocytopenia was found in 83.8% in dengue fever, 93.8% in DF with
warningsignsand81.5%inseveredenguefeveranditisnotstatisticallysignificant(p=0.308).

Dengue virus, a mosquito-borne flavivirus, is the causative agent, currently one of the most
significant emerging disease challenges to global health. The dengue virus are single-stranded
positive-sense RNA viruses with a genome of about 11000 bases that codes for three
structural proteins, C(core protein), M (membrane protein), E (envelope protein); 7
nonstructural proteins, NS1, NS2a, NS2b, NS3, NS4a, NS4b, NS5; and short non-coding
regions on the 5' and 3' ends. This was a case control study design in which 201 cases of
dengue fever admitted in the pediatric emergency ward, were enrolled. After taking written
informed consent, data were collected in a predesigned semi structured questionnaire
regarding Socio-Demographic profile, medical history, clinical and hematological profile and
outcome. 8(4%) of cases had positive family h/o dengue fever, 3(1.5%) cases had positive
past h/o dengue fever and 8(4%) cases had positive h/o travel to dengue endemic areas.
201(100%) cases had fever, 94(46.8%) had persistent vomiting,91(45.3%) had
anorexia,78(38.8%) had abdominal pain, 59(29.4%) had headache, 30(14.9%) had rashes, 16
(8%) had facial puffiness, 14(7%) had convulsions and 12(6%) had abdominal distension.