Online ISSN: 2515-8260

Keywords : 3D printing

Cytotoxic evaluation of directly 3D printed aligners and Invisalign

S Fayyaz Ahamed; S Mohnish Kumar; R K Vijaya kumar,; A S Apros Kanna; K Indrapriya dharshini

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 5, Pages 1129-1140

Background: Direct 3D printing of aligner trays involve printable materials; the study aims to investigate the in-vitro cytotoxicity of the direct-printed aligner using photopolymer resins and SmartTrackInvisalign tray for varying time intervals on 3T3 mice fibroblast cells using MTT assay.
Materials and Methods: Directed printed aligner trays using two 3D printing materials with SmartTrackInvisalign tray were compared in this study. Samples were placed in Dulbecco’s Modified Eagle’s Medium (DMEM; 0.1 mg/mL) for 1,3,5& 7 days interval. Cell viability percentage was calculated, and data were analyzed using a one-way analysis of variance and post hoc tests (α = 0.05).
Results: All materials exhibited slight cytotoxicity on MFCs with a visible trend of a significant increase in cell viability from day 1 to 7. Among the groups, the higher cytotoxicity was by E-Guard clear, and Dental LT, and the least cytotoxicity by Smartrack material. The highest level of cell viability and no cytotoxicity was exhibited by Invisalign (94.07% ± 3.00 of cell viability) at day 7. No statistically significant difference in viability percentage was seen between Dental LT and E-Guard material.
Conclusions: SmartTrackInvisalign material (polyurethane) was found to be more biocompatible, followed by directly printed aligner materials (polymethylmethacrylate). Cytotoxicity was found to be more on the first day for all materials and gradually decreases as day’s progress. The results indicate the increased leaching of material during the initial period of use though the level of cytotoxicity is slight.

A Review on surface enhancement approaches for thermoplastics developed through Fused Deposition Modeling

Vinay Shah; Raman Kumar; Jasgurpreet Singh Chohan

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 7, Pages 4485-4497

As the Demand of low cost, tailor-made products are increasing in the contemporary
industries, the research and development of different material processing techniques has
been intensified. Fused deposition modeling (FDM) is most successful and popular
additive manufacturing technique which used thermoplastic polymers as raw materials.
This paper aims to study and analyze the fundamental working process, applications,
limitations and challenges for FDM technology in present and future. Poor surface finish,
which is one of intrinsic defect of this technology, has been considered as major barrier
against implementation in critical application areas. Various chemical, mechanical and
pre-processing techniques are elaborated along with detailed literature review. The impact
of finishing operations in materials properties and overall cost of product has also been
discussed in detail. The study also discussed the current and future challenges along with
remedies which would help this technology to sustain in competitive environment


Neha N; Dr. Jayalakshmi Somasundaram; Dr. Subhabrata Maiti; Dr.Jessy P

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 1482-1497

3D printing otherwise known as additive manufacturing, rapid prototyping or layered manufacturing is a relatively new, quickly growing and rapidly expanding method of manufacturing that has got numerous applications in healthcare and also in many other fields. Recently, it has become a subject of great interest in planning surgeries. Additive manufacturing method involves the production of a 3D model by laying down or adding successive layers of material. 3D printers are equipment that produces 3D models using CAD technology or 3D scanners. It has received more importance with the advancement in 3D imaging and modelling technologies such as CBCT, intraoral scanning and CAD/CAM in dentistry. Different techniques are employed in 3D printing namely stereolithography, photopolymer jetting, power binder printers, direct light processing, selective laser sintering, fused deposition modelling, electron beam melting, etc. Dental laboratories are able to produce 3D printed restorations, crowns, bridges, orthodontic appliances, surgical guides and implants quickly with higher precision and accuracy. This is done by methods that combine oral scanning, CAD/CAM designing and 3D printing. The rate of success of 3D printing has improved the quality and accuracy of dental treatment. With the application of 3D printing, it has become possible to replicate the desired complex geometry which was not feasible with conventional techniques. Thus 3D printing has led to a transformation in digital dentistry with its extensive learning and penetrating opportunities and a wide range of applications. The aim of this article was to review the techniques and current applications of 3D printing in dentistry.

Abstracts: 5th Annual Congress of the European Society for Translational Medicine (EUSTM-2017), 20–22 October 2017, Berlin, Germany

Aamir Shahzad; Randall J. Cohrs

European Journal of Molecular & Clinical Medicine, 2017, Volume 4, Issue 1, Pages 48-98

Regenerative medicine is a promising field with the potential to overcome the increasing need for donor organs either by stopping disease progression (e.g. with cells, genes or biologics) or by providing novel organ options. Furthermore, regenerative medicine strategies are unlike other treatments in that they are meant to persevere and treat the underlying injury rather than symptoms. This requires a level of persistence and safety and long term efficacy not always previously required for new therapies. In the past decade, clinicians have been able to utilize cell and gene therapies in unprecedented numbers, but with mixed results. At the same time, scientists have engineered organs (bladder, esophagus and blood vessels) that are considered simple structurally and functionally. However, regenerative medicine is yet to fully succeed with cells or genes or to fabricate fully functional solid organs such as kidneys, livers, lungs, and hearts. Yet, development of organs in the laboratory is proceeding both via 3D printing and use of decellularized scaffolds