European Journal of Molecular & Clinical Medicine

To Study the presence of micronucleus in urothelial cells of patients with normal and abnormal cervical visual inspection and to correlate with cytological and histological findings in normal and various intraepithelial lesions of cervix and cervical carcinoma. To assess the role of urothelial MN score in predicting cervical cancer risk.
Materials and Methods: A total of 350 patients with the complaints of post coital bleed, lower abdominal pain, menstrual irregularities who come in gynaecological out patient department of Rajindra Hospital and Medical College, Patiala were included in our study. Patient’s cervix was visually examined, pap smear taken and midstream urine sample collected for MN scoring. Incidence of micronucleus in urothelial cells recorded as micronucleus cells per 1000 cells by 4 cytopathologists.
Results: The mean Urothelial MN score ± Standard deviation was found to be 2.80±1.58 in inflammatory cases,4.55±1.63 in ASCUS cases,5.67±0.58 in ASC- H cases ,5.61±0.50 LSIL cases, 6.09±0.30 in cases of HSIL,6.50±0.53 in invasive carcinoma. A step-wise increase was observed in MN score from inflammatory to IC categories.
Conclusion: Urothelial MN score is a reliable and easy test that can be used along with routine cervical PAP to assess the risk of premalignant  and  malignant transformation in the cervix as a biomarker for predicting the risk of carcinoma.

Background: Cervical carcinoma is second most common cancer in India and a major
cause of death among women.Timely diagnosis and treatment allow significant reduction
of morbidity and mortality.The present study is an attempt to screen women attending
Gynaecology OPD at Index Medical College Indore for the cervical cancer using Pap
smear studies.
Objective: To evaluate the use of Pap smear screening method for detection of precancerous
lesions of the uterine cervix.
Methodology: In this study 110 women attending the Gynae OPD with complaints of
abdominal pain, irregular menses, discharge PV and post-coital pain or bleeding were
included. Pap smear was taken after history taking and clinical examination for all women
to screen the cervical cancer. Smear stained with Papanicolaou stain and studied in the
Department of Pathology.
Results: In the study, most women were in the age range of 21 to 50 years and
multiparous.Vaginal discharge was the chief complaint,followed by irregular menses and
lower abdominal pain .After Pap smear study,the test was negative for malignancy in 23 %
of women, 40% of women had bacterial vaginosis and 18% diagnosed as having
candidiasis.ASC-US, ASC-H, LSIL and HSIL were detected in 5.5%, 2%, 5.5% and 2 % of
women respectively.Women with ASC-US, ASC-H, LSIL, HSILunderwent colposcopy and
biopsy.
Conclusion: Pap smear is a simple, non-invasive, cost-effective, highly sensitive and
specific method for diagnosing precancerous lesions of cervix.By instituting a well-planned
screening program, cervical cancer related morbidity and mortality can be reduced