Online ISSN: 2515-8260

Keywords : LDH


Correlation of Clinical, Hematological and Biochemical Parameters in Women with Severe Preeclampsia and Maternal Outcome: An Observational Study

Guruprasad Hosamani,Dr. Niranjan C. S., Anuja Sagamkunti, Sushrit A. Neelopant, Rashmi M. B

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 292-301

Background: To find correlation of clinical, hematological and biochemical parameters
in women with severe preeclampsia and maternal outcome and to study the maternal
and perinatal outcome in severe preeclampsia and.
Materials and Methods: The study was done in the Department of Obstetrics and
Gynaecology, Raichur Institute of Medical Sciences, Raichur. 140 patients with blood
pressure ≥ 160/110 mm of hg with proteinuria or Blood pressure ≥ 140/90 mm of hg
with proteinuria of ≥ 2+ were involved in the study. Statistical analysis was done by
applying chi-square.

An Observational Study of the Effect of Laparoscopic Cholecystectomy on Liver Function Test

Hari Om Dhaker, Shyam Bhutra, Yogendra Singh Chundawat, Naresh Kumar, Nani Dhaker, Saranshi Singh

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 2308-2314

Background: Cholecystectomy is a common treatment of symptomatic gallstones and other gallbladder conditions. As it gained worldwide popularity, it has become one of the most common operations performed in general surgical practice. In this study we aimed to investigate to determine changes in liver function tests after laparoscopic cholecystectomy.
Material & Methods: A prospective observational study done on 100 patients who underwent elective laparoscopic cholecystectomy were taken up for the study in the department of General surgery at JLN medical college and hospital, Ajmer, Rajasthan, India during period between January 2020 to December 2021. All patients who gave consent for the study underwent a standard clinical and laboratory evaluation including USG. Pre-operative investigations included liver function tests i.e. prothrombin time, bilirubin (total and direct), alanine transaminase, aspartate transaminase, alkaline phosphatase, total proteins, serum albumin, GGT, and LDH. The subjects under inclusion criteria have taken up for the study. The liver function tests have repeated 24 hours, 3rd day, 7th days, 10th days after the operation and compared.
Result: The mean age of the study population was 43.3 years with a standard deviation of 13.35 yrs. A negative t-STAT shows that the value of serum total protein, serum albumin was falling after the laparoscopic procedure. A statistically significant increase in the bilirubin(total and direct), SGOT, SGPT, GGT, LDH, and serum alkaline phosphatase levels were noted after laparoscopic cholecystectomy and no statistical significance in prothrombin time.
Conclusion: We concluded that statistically significant increase in the bilirubin (total and direct), SGOT, SGPT, GGT, LDH, and serum alkaline phosphatase levels after Laparoscopic cholecystectomy. There was a decrease in total protein and albumin and no change for prothrombin time. However, further studies with larger sample sizes and multi-center trials would yield more definite results, along with meta-analyses. We strongly suggest the consideration of confounding factors such ascomorbidities, duration of surgery, CO2 pressure, utilized anesthetic agents, and also longer period follow-ups. 

To determine the relationship between biochemical indicators and the severity of COVID-19

Dr Saleha Shaheen

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 5861-5865

Aim: To determine the relationship between biochemical indicators and the severity of COVID-19.
Methods: Blood samples were obtained by competent doctors and nurses in accordance with ICMR
guidelines. The samples were then forwarded to the central biochemistry laboratory for additional
analysis. The Access-2 completely automated chemical analyzer was used to test ferritin, quantitative
CRP, and IL-6, while the AU 480 analyzer was used to assess LDH.
Results: We included 200 confirmed covid-19 patients in our trial. All of the patients were above the
age of 18. The prevalence of HTN, diabetes, CKD, COPD, and Cardiac Disease in the study patients
was 40%, 32%, and 15%, respectively. 20 and 30 percent, respectively. In the current research, the
mean values of IL-6, ferritin, CRP, and LDH were shown to be higher in covid patients, with the rise
being greater in patients on intubation. The mean and standard deviation of biochemical parameters in
non-ICU, ICU, and intubation patients.
Conclusion: Serum CRP, LDH, IL-6, and ferritin levels that are increased may be employed as
laboratory indicators for a bad prognosis in COVID-19.

Lactate dehydrogenase as an indicator of liver tumors

Dr. Akshatha LN, Dr. Sandeep Kumar, Dr. Ramesh G3, Dr Rachana, Dr. Ramchandra C

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 1, Pages 826-830

Background: Lactate dehydrogenase (LDH, L-lactate, NAD+ oxidoreductase, EC1.1.1.27) is a
family of at least six NAD + -dependent isoenzymes (LD1-LD5 and LD6/LDX). It is recognized
as being one of the most common enzymes in nature. It belongs to the class of oxidoreductases
and it is characteristic for the final stage of anaerobic glycolysis. Aim of the study: To evaluate
the efficacy of lactate dehydrogenase as an indicator of liver tumors. Materials and methods:
The present study was performed in the Department of Biochemistry, Kidwai Institute of
Oncology, Bengaluru, Karnataka, India. For the study, a total of 100 patients were selected. The
age of the patients ranged from 18 to 40 years. Briefly, 20 μL of each patient serum was mixed
first with 1000 μL of buffer (pH 7.35) supplied by the manufacturing company and incubated for
1-5 min at 25 °C. A volume of 250 μL of substrate that composed of nicotinamide-adenine
dinucleotide (0.75 mmol/L) and sodium azide (0.095%) was added to the mixture. After mixing,
the absorbance at 340 nm was read by UV-visible spectrophotometer (CECiL, CE 1021,
England) after 1 min. CK levels were assayedaccording to Humazym M-test as described by the
instructions of the produced company. Results: It was observed that number of male patients
was 45 and number of female patients was 55. The mean age of the participating patients was
31.35 years. High LDH level are observed in large number in females (30 patients) than in males
(24 patients). Increased LDH levels in blood could not be indicator for liver diseases only, but it
may be resulted from damage in muscular tissues. 30 females and 24 males who possessed high
level of LDH were investigated for CK, as monitor for muscular injury. Additionally, 6 male
patients and 9 female patients showed increased levels of LDH and CK in their blood which
means they may sever from muscular damage. Conclusion: From the results of the present study,
this can be concluded that LDH can be used as a good biomarker for diagnosis of liver,
muscular and even cancer diseases. There is little variable between males and females in the
elevated values of LDH. Patients with normal values of CK and high level of LDH suggested to
have unidentified cancer disease, except liver cancer, and for that other specific tests are
required.