Online ISSN: 2515-8260

Keywords : peripheral blood smear


Comparison of anemia characterization between automated CBC analysis and conventional peripheral blood smear assessment

Dr. Mehak Kashyap, Dr. Naveen Kakkar, Dr. Neelam Gupta

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 2, Pages 380-388

Introduction: Complete blood count (CBC) by the automated hematology analyzers and microscopic
examination of peripheral smears have traditionally been used in the diagnosis of anemias. The advent
of automated hematology analyzers has improved accuracy and precision of test results and has reduced
subjective errors. This study aimed at
i) Comparing anemia characterization between automated CBC analysis and conventional peripheral
blood smear assessment.
ii) Studying RBC histogram patterns in various categories of anemia.
Materials and methods: Blood samples from 500 adult anemic patients were run in Sysmex XP-100
fully automated, 3-part differential hematology analyzer. In all patients, blinded peripheral blood smear
examination by two observers was done. Anemia categorization by peripheral blood smears and
automated red cell data was compared.
Results: The number of patients with normocytic normochromic anemia on automated CBC was 280
and on peripheral smear examination was 269. On automated CBC, 137 patients had microcytic
hypochromic anemia whereas 107 patients had microcytic hypochromic picture on microscopy.
Significantly higher number (p<.05) of patients (76) with microcytic normochromic morphology was
diagnosed on blood smear compared to automated counts (17). When RBC volume and hemoglobin
content were considered together, a Kappa value of. 447 was obtained indicating moderate agreement
between the automated and manual (peripheral blood smear) assessment of anemia.
Conclusion: Patients with most anemia types can be accurately diagnosed by automated CBC analysis.
The peripheral blood smear has limitations in cases with borderline MCVs and mild hypochromia which
may be missed. It, however, still remains the cornerstone in the identification of abnormal RBC
morphology seen in hemolytic anemias.

Study of Pancytopenia in Peripheral Blood Smears

Samir Ranjan Bhowmik, Ranbir Singh Chawla, Ramanuj Singh

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 2730-2733

Background:Pancytopenia is decrease in all the cellular constituents of the peripheral blood resulting in anaemia, leukopenia, and thrombocytopenia. Pancytopenia is characterised by anaemia, bleeding, and infection. The causes of pancytopenia vary widely. Hence, the present study was conducted to assess the profile of pancytopenia and its various causes.
Materials and Methods: The present cross-sectional observational study was conducted in Department of Pathology of a medical college hospital. A total of 40 cases of anaemia suffering from pancytopenia were included. Detailed history, clinical examination and investigations were done to determine the cause of pancytopenia.
Results: Most of the patients belonged to the age group of 21-30 years (37.5%). 57.5% were males. Megaloblastic anaemia was seen in 57.5% cases, hypoplastic/aplastic anaemia in 17.5% and hyersplenism in 7.5%.
Conclusion: Megaloblastic anaemia and aplastic anaemia were major causes of pancytopenia. Proper investigation is must in patients of pancytopenia