Online ISSN: 2515-8260

Keywords : antioxidant enzymes


Oxidative Stress And Enzymatic Antioxidant Defense Systems With Special Reference To Vitamin E Supplementation To The Sedentary Exercising Females.

Chandana Bera, Dr. Manila Jain, Subarna Ghosh, Bijay Kumar Mahaseth

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 501-507

Background: Exercise increases the production of free radicals, while vitamin E acts as a
scavenger of free radicals, while vitamin E acts as a scavenger of free- radicals. Thus exogenous
vitamin E supplementation has been shown to be effective against exercise- induced oxidative
stress.The study aims to investigate the effect of vitamin E (400mg) supplementation on the
endogenous antioxidant systems of the body viz. superoxide dismutase (SOD), glutathione
peroxidase (GPX) and catalase (CAT) of hundred healthy sedentary females of 18-21 years age
group and comparable height and weight. Material & Methodology: Endurance capacity of
each subject was determined by exercising them on Magnetic Break Bicycle Ergometer at a fixed
workload of 600KgM/ min till exhaustion. SOD, GPX and CAT levels were analyzed at pre and
post exercise levels. The subjects were divided into two groups --- control group (n=50) and the
group receiving 400 mg of vitamin E (Evion 400) (VE) supplementation for 15 days. The same
experimental procedure was repeated after the supplementation tenure. Results :Results
indicates that exercise caused a significant decrease in the CAT level (P<0.05) and an increase in
SOD level (P<0.001) and GPX level (P< 0.05). However, these changes in the level of SOD, GPX
and CAT were minimized after exogenous vitamin E supplementation.Conclusion: The entire
result signifies that vitamin E is highly effective in combating exercise- induced oxidative stress
in sedentary females.

Impact of Beta carotene and antioxidant minerals supplementation in combination on the exercise induced oxidative stress in sedentary females.

Subarna Ghosh, Chandana Bera, Dr. Manila Jain

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 5216-5222

Background: Physical exercise may be associated with a 10-20 fold increase in whole body oxygen
uptake. Oxygen flux in the active peripheral skeletal muscle fibers may increase by as such 100 to 200
fold during exercise. Studies during the past 2 decades suggest that during strenuous exercise, generation
of reactive oxygen species (ROS) is elevated to a level that overwhelms tissue antioxidant defense
systems. The result is oxidative stress. The present study aims to investigate the effect of supplementation
of beta carotene, minerals and antioxidants in combination on the endogenous antioxidant system of the
body (viz., glutathione peroxidase GPX, super oxide dismutase SOD and catalase CAT) of hundred
females of 18-21 years age, and comparable height, and weight. Methods: Endurance capacity (min) of
each subject was determined through exercise on a Magnetic Break Bicycle Ergometer at a fixed
workload of 600 kgM/min till exhaustion. GPX, SOD and CAT levels were analyzed at pre and post
exercise levels. The subjects (N=100) were divided into two groups – control group (n=50) receiving
placebo supplementation and the experimental group (VM+Se) receiving supplementation of beta carotene
and antioxidant minerals combinedly in capsular form (n=50) for 15 days. The same experimental
procedure was repeated after supplementation. Results: Results indicate that significant increase in
endurance capacity (P<0.001) and post exercise significant decrease in GPX (P<0.05) and SOD (P<0.001)
and no significant changes in CAT (P>0.05) after beta carotene and antioxidant minerals combinedly
supplementation. Conclusion: Beta carotene and antioxidant minerals seem to be beneficial to control
oxidative stress and enhances endurance capacity.