European Journal of Molecular & Clinical Medicine

Pseudomonas aeruginosa is identified as an opportunistic pathogen since it predominantly creates nosocomial infections in immune compromised people; total of 20 samples of P. aeruginosa obtained from a variety of locations in Baghdad hospitals isolated from: burns, wounds and sputum collected from October 2021 to the April 2022, isolates cultured by Using culture conditions, biochemical assays, and the VITEK-2 compact system. For all bacterial isolates, Biofilm production was detected using the micro titration plate technique, with the majority of strains (50%) producing moderate biofilms, 35% producing strong biofilms, and 15% producing weak biofilms. When it comes to virulence genes, In 20 isolates, three genes were identified, the algD gene was found in 17 isolates (85%), pelA was found in 17 isolates (85%), and pslD was found in 15 isolates (75%). The genes algD, pslD, and pelA were chosen as virulence factors, the genotypic algD/pslD/pelA – pattern, which is responsible for the phenotypic pattern of biofilm development. There were statistically significant variations in genotypic pattern predominance across biofilm forming isolates from various sources, Using Chi-square analysis, a very significant relationship between biofilm forming capability and genetic pattern (p value 0.764) was discovered

Introduction: Biofilm protects Enterococci from host immune response and antibiotics.
Biofilm-producing Enterococci cause recurrent, chronic, and antibiotic-resistant
infections. According to the National Institute of Health, 80% of infections are related to
biofilm-forming microbes. Apart from biofilm-forming ability, Enterococcus spp. are
known to produce various virulence factors. Biofilm formation is a major mechanism of
adaptation that protects bacteria from antibiotics, due to several characteristics.
Materials and Methods: This is a prospective, descriptive and observational study
conducted in the Department of Microbiology, Index Medical College, Hospital and
Research center Indore from January 2019 December 2021. All isolates of Enterococcus
species during the study period will be included. Enterococci isolated from clinical
specimens like pus, wound swab and aspirates etc. received in Microbiology
Department. All samples were processed by standard bacteriological procedures. Gram
staining was done for pus, wound swab and aspirates and findings were recorded.
Culture was done on 5% sheep blood agar and MacConkey agar. Inoculated plates were
incubated at 37°C for 18- 24 hours.