Online ISSN: 2515-8260

Keywords : conventional smear


COMPARATIVE EVALUATION OF THE DIAGNOSTIC ACCURACY OF CONVENTIONAL SMEARS TO LIQUID-BASED CYTOLOGY FOR BREAST LESIONS

Dr. Harsh Jakhetia, Dr. Smriti Pandey, Dr. Kamlesh Patel, Dr. Dhruvendra Pandey

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 1019-1026

Background: For the evaluation and assessment of both non-gynaecologic and gynaecologic
specimens, extensive use of LBC (Liquid-based cytology) has gained popularity in the recent
past. Better assessment morphologically is possible owing to the monolayer cell suspension.
Also, LBC allows assessment of nucleolar prominence, smaller cell size, fragmented cell
clusters, lost, reduced, or altered background material.
Aim: The present study was conducted to comparatively evaluate the utility and efficacy of
Liquid-Based Cytology over conventional smears for assessment of the breast lesion, and to
assess their use as an alternative to the conventional preparation.
Materials and Methods: The present prospective clinical study included 374 female subjects
of the FNAC (fine-needle aspiration cytology) obtained from subjects having palpable breast
lesions. The second pass was given, whereas, the first pass was used for either LBC or
conventional smear. Several criteria were used to compare representative LBC and
conventional smear. Individual scoring of each criterion was done followed by statistical
evaluation.
Results: The study results showed that statistically non-significant difference was seen
between LBS and conventional concerning nuclear and cytoplasmic details, presence of
monolayer,cytoarchitectural pattern, and cellularity, whereas, a statistically significant
difference was seen for background blood-debris and informative background.

Study of cell block technique in the cytodiagnosis of serous fluids at a tertiary hospital

Ashvini Pandit Mane, Hemant R. Kokandakar, A.R. Joshi

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 12216-12225

Background: Conventional smear is a less complicated procedure than that of cell block technique, it has lower sensitivity due of overcrowding of cells, cell loss, lack of architecture and also abundance of inflammatory cells and scarcity of representative cells contribute to appreciable difficulties in creating conclusive diagnosis on conventional smears. The aim of this study was to check the diagnostic yields of cell block technique in the cytodiagnosis of serous fluids.
Material and Methods: Present study was cross-sectional observational study, conducted in samples of ascitic fluid, pleural fluid, pericardiac fluid that were received in the cytology section, collected from patients clinically and radiologically diagnosed as having serous effusion. After reporting the conventional cytological smear the representative received samples were processed for cell block preparations.
Results: Findings of cellularity differs significantly, indicating better yield of cellularity in cell blocks as compared to conventional smear method. Distribution of findings of background obscured by blood or proteinaceous material differs significantly, indicating less degree of background obscured by blood or proteinaceous material on cell block preparation. Distribution of findings of morphology differs significantly, morphology is better appreciated with the help of cell blocks as compared to conventional smear method. Distribution of findings of architecture differs significantly, indicating better appreciation of architecture on cell blocks. Conclusion: Cell block method, concentrates cellular rich material, and it can increases cellular yield for diagnosis. Cell block increases cellular architecture pattern like acini, cells in sheets, papillae, which has helped to identify possible type or primary site of malignancy