Online ISSN: 2515-8260

Keywords : fine-needle aspiration cytology

A study of the role of electron microscopy in fine needle aspirates of neoplastic lesions

Lt Col Shivani Sangwan, Col Prabal Deb, Lt Col Preeti Patni, Brig N S Mani

European Journal of Molecular & Clinical Medicine, 2023, Volume 10, Issue 1, Pages 65-78

Introduction: Evolution of cytopathology in the past few decades as a distinct adjunct in screening and diagnosis of a neoplastic process, has paralleled the development of newer ancillary techniques like immunocytochemistry, flow cytometry, karyotyping and genetic studies. But a combination of LM and IHC will not identify every tumor and some diagnostic problems go unanswered. If the differential diagnosis on routine LM includes a tumor that is known to possess distinctive ultra structure, Electron Microscopy (EM) is clearly the method to select for confirmation as a subsidiary technique.
Aim: Owing to the restricted availability of TEM in most centres,  data pertaining to its utility in tumor cytopathology is limited. Hence, the current study is designed to study the role of Electron Microscopy (EM) in fine needle aspiration cytological examination of neoplastic lesions, to correlate the findings of EM with Light microscopic findings and to correlate the EM findings with immunohistochemical findings, wherever possible.
Materials and Methods: Electron microscopy was carried out in a
total of 50 cases where were subjected to FNAC for suspected neoplastic primary malignant or metastatic lesions. The cellular features of material aspirated were studied and ultrastructural details of cytoplasm including the plasma membranes, organelles and nuclear features were noted. The cases were grouped histogenetically and analysed. The final cytological diagnosis were tabulated and compared with the histopathological diagnosis.


Dr. Harsh Jakhetia, Dr. Smriti Pandey, Dr. Kamlesh Patel, Dr. Dhruvendra Pandey

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 1019-1026

Background: For the evaluation and assessment of both non-gynaecologic and gynaecologic
specimens, extensive use of LBC (Liquid-based cytology) has gained popularity in the recent
past. Better assessment morphologically is possible owing to the monolayer cell suspension.
Also, LBC allows assessment of nucleolar prominence, smaller cell size, fragmented cell
clusters, lost, reduced, or altered background material.
Aim: The present study was conducted to comparatively evaluate the utility and efficacy of
Liquid-Based Cytology over conventional smears for assessment of the breast lesion, and to
assess their use as an alternative to the conventional preparation.
Materials and Methods: The present prospective clinical study included 374 female subjects
of the FNAC (fine-needle aspiration cytology) obtained from subjects having palpable breast
lesions. The second pass was given, whereas, the first pass was used for either LBC or
conventional smear. Several criteria were used to compare representative LBC and
conventional smear. Individual scoring of each criterion was done followed by statistical
Results: The study results showed that statistically non-significant difference was seen
between LBS and conventional concerning nuclear and cytoplasmic details, presence of
monolayer,cytoarchitectural pattern, and cellularity, whereas, a statistically significant
difference was seen for background blood-debris and informative background.