Online ISSN: 2515-8260

Keywords : antimicrobial susceptibility

A Study of Aerobic Bacteriological Profile and Antimicrobial Susceptibility Pattern of Isolates in Patients with Chronic Osteomyelitis at Tertiary Care Hospital

Abhishek Binnani, Yogendra Taneja, Prabhakar Kumawat, Bhagirath Ram Bishnoi, B P Sharma, Anjali Gupta, Kirti Shekhawat

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 2210-2217

Introduction:  In recent times, there has been change in bacteriological profile of chronic osteomyelitis cases as well as susceptibility of antibiotics.
Objectives: 1. To study Demographic characteristics of Chronic Osteomyelitis cases, 2. To isolate causative Aerobic bacteria (other than Mycobacteria) 3. To analyze Antimicrobial Susceptibility Pattern of isolated aerobic bacteria.
Methodology: A cross sectional hospital-based study of 1 year duration was conducted at Department of Microbiology, S. P. Medical College, Bikaner, Rajasthan. After obtaining informed verbal consent, 110 chronic osteomyelitis cases were recruited to study following nonprobability consecutive sampling. Their pus samples were taken and studied for staining, presence of enzymes, biochemical reactions, fermentation tests, antibiotic susceptibility testing. Obtained results were analysed and presented through tables and diagrams.
Results: 50% cases had S. aureus and 18% had P. aeruginosa in their pus samples. Majority of patients were male and almost 1/3rd cases belonged to 16-30 years age group. S. aureus isolates were sensitive to Cotrimoxazole & Linezolid. Most of the isolate of Klebsiella pneumoniae (83.33%) were sensitive to Meropenem. All isolates of Pseudomonas aeruginosa (100%) were sensitive to Polymyxin B and Colistin C.
Conclusion: Study of bacteriological profile and antimicrobial susceptibility pattern helps significantly in clinical management decisions.

To evaluate the bacteriological profile and antibiotic sensitivity pattern of isolates from blood culture

Dr. Pratibha Sharma,Dr. Ranganath T Ganga

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 5872-5878

Aims:To evaluate the bacteriological profile and antibiotic sensitivity pattern of isolates from blood
Methods: In all, 610 patients with a suspected blood stream infection were recruited for this research
from the different OPDs, IPDs, and ICUs located within the hospital. Standard microbiological
procedures, including colony character, gram staining, motility testing, and standard biochemical
tests, were used throughout the process of isolating and identifying the organisms. Antimicrobial
susceptibility test Kirby Bauer's disc diffusion technique was used on Mueller Hinton agar (MHA) in
accordance with the recommendations provided by the CLSI to test antimicrobial sensitivity.
Results:During the course of this investigation, a total of 610 blood samples were obtained for the
purpose of blood culture; of them, 120 (19.67 percent) were culture positive. From a total of 120
positive cultures, 49 were gram-positive bacteria (40.83 percent), 64 were gram-negative bacteria
(53.33 percent), and 7 were Candida spp. Klebsiella species, which accounted for 36.37 percent of the
total 120 isolates, was the most prevalent organism, followed by Staphylococcus aureus, which
accounted for 18.33 percent, and Escherichia coli, which accounted for 2.5 percent of the total.
Vancomycin (90.91 percent) and linezolid (90.91 percent) exhibited the greatest activity when
compared to the other antibiotics that were utilised for susceptibility testing for gram-positive isolates.
Conclusion:Klebsiella species was the most prevalent organism, followed by Staphylococcusaureus
as the second most prevalent organism. Imipenem was the medicine that was shown to be the most
effective against gram-negative bacteria, while Vancomycin and Linezolid were found to be the most
effective against gram-positive bacteria.