Online ISSN: 2515-8260

Keywords : VesiculoBullous lesions

Clinicopathological Study of Non Infectious Vesiculobullous lesions of Skin and Mucous Membranes

Dr. Nisha H Mehta, Dr Premnath N Hiryur

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 4, Pages 1885-1910

INTRODUCTION: The vesiculobullous lesions of the skin are a group of heterogeneous diseases characterized by the formation of vesicles/blister and are associated with variable clinical manifestations and have multiple causes.
The immunobullous disease are mainly a consequence of pathogenic autoantibodies which target those antigens which mainly function to maintain either cell to cell adhesion within the epidermis or adhesion of stratified squamous epithelium to dermis or mesenchyme.
MATERIALS AND METHODS: The study was prospective and observational case series carried out in the Department of Pathology, SBKS MI&RC, Sumandeep Vidyapeeth, Piparia, Vadodara in the time period of January 2019 to July 2020. In the study 30 punch biopsies of patients taken from their cutaneous or mucocutaneus lesions were fixed, processed and stained stained with Hematoxylin and Eosin and examined light microscopy.
RESULT: Total 30 cases as per inclusion criteria were included in present study. Peak incidence is seen in the age group of 40-50 years with a slight female prepondarance. The most common morphological presentation was in the form of blister/vesicle. Pemphigus vulgaris is the most common lesion(47%) encountered followed by Pemphigus Foliaceous in 3 (10%) cases and Bullous Pemphigoid in 3 (10%) cases, Dariers Disease and Hailey Hailey Disease showed 2 (6.6%) cases each, a single case each of Epidermolysis Bullosa Simplex, Dermatitis Herpetiformis, Irritant Contact Dermatitis and Pemphigus Herpetiformis.
CONCLUSIONS: The highest incidence of vesiculobullous disorders is seen in the age age group of 40- 50 years and M:F ratio being 1:1.14. Pemphigus Vulgaris is the most prevalent disorder. On light microscopy, the most common site of blister formation is suprabasal. The diagnosis could be made on the basis of clinical features or histopathological features alone (70%) or their correlation in 90% of the cases.


V. SaiAbhishek, KattaRamya, NugalaSindhura, Rayapa Reddy T, D. Krishna Dharani

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 4, Pages 2855-2861

Background: Skin  is  the  largest  organ  of  the  body  and  various diseases  can commonly  involve  the  skin  out  of  which vesiculobullous  lesions  form  one of predominant  group. Histopathological study of bullous skin lesions andto knows the incidence of bullous lesions and to study bullous lesions in relation to age and sex.
Materials and Methods: A prospective study of vesiculobullous disorders were studied over aspan of 24 months from July 2019 to June 2021.  A total of 48 skin biopsies from patients with vesiculobullous skin lesions were sent to the Department of Pathology, Guntur Medical College, Guntur. Punch biopsies were taken for histopathological diagnosis. H& E staining was done.
Results: In the present study pemphigus foliaceus is the most common vesiculobullous disorder (35.41%) followed by pemphigus vulgaris in 18.75% of cases. Majority of patients presented between 31-40yrs of age with female prepondarance.  Subcorneal blister was noted in pemphigus foliaceus and suprabasal blister was seen in pemphius vulgaris.Dermoepidermal junction separation was seen in bullous pemphigoid, dermatitis herpitiformis, and bullous SLE.
Conclusion: Vesiculobullous disorders represent a heterogeneous group of dermatoses with female preponderance and peak incidence inthird decade. Pemphigus foliaceus constituted the most common subtype of vesiculobullous disorder in this study followed by Pemphigus vulgaris.

Role of Tzanck Smear microscopy for detection of vesiculobullous skin lesions and active genital herpes- a pilot study in tertiary care hospital, Patna, Bihar

1Randhir Kumar, 2Kranti C Jaykar, 3Nidhi Prasad, 4Shailesh Kumar, 5Rakesh Kumar, 6Namrata Kumari .

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 3326-3331

Background: Cytology is a diagnostic technique used to evaluate the properties of individual cells. Tzanck smear is a quick, simple, sensitive cytology procedure that can be done with little patient discomfort and expense.
Objective: The purpose of this study was to determine the value of Tzanck smear in evaluating vesiculobullous illnesses and active genital herpes in relation to clinical diagnosis.
Methods: A cross-sectional study was carried out to determine the value of Tzanck smear in evaluating various vesiculobullous skin lesions in comparison to clinical diagnosis. For statistical analysis, the Fisher's exact test was applied.
Results: The majority of the 70 patients evaluated had viral infections or auto immune vesiculobullous diseases. On cytology, all patients with viral infection had multinucleated giant cells, and all patients with pemphigus had a large number of acantholytic cells as well as an inflammatory infiltrate. All of the patients with bullous pemphigoid had a significant number of eosinophils. The presence of acantholytic cells, multinucleate large cells, and eosinophils had a highly significant connection with clinical diagnosis.
Conclusion: Tzanck smear, while not a replacement for traditional histology, can aid in establishing the clinical diagnosis with ease and speed.