Online ISSN: 2515-8260

Keywords : gold nanoparticles

Gold Nanoparticles In Diagnosis & Management Of Oral Cancer & Pre-Cancerous Lesions.

Dr. Isha Balmuchu, Dr. Basavaraj T Bhagawati, Dr.Nishant Kumar, Dr Sharanamma B, Dr. Jyotsana

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 7, Pages 9263-9267

Gold is one of the first metals to have been discovered; the history of its study and application spans at least several thousand years. In particular, the optical properties of GNP are determined by their plasmon resonance, which is associated with the collective excitation of conduction electrons and localized in the broad region. The application of nanotechnology for the treatment of cancer is mostly based on early tumor detection and diagnosis by nanodevices capable of selective targeting and delivery of chemotherapeutic drugs to the specific tumor site. The nanotechnology‑based detection and diagnostic methods used are Magnetic resonance imaging (MRI), Optical coherence tomography (OCT), Photoacoustic imaging, Surface plasmon resonance scattering, Surface‑enhanced Raman spectroscopy, Diffusion reflection imaging, Quantum dots imaging, and Nano‑based ultrasensitive biomarker detection. Due to the remarkable properties of gold nanoparticles, they have long been considered a potential tool for the diagnosis of various cancers and for drug delivery applications. Recent advances in nanomedicine make it auspicious for cancer diagnosis and treatment. The attractive properties of gold nanomaterials, particularly, anti-angiogenic properties, are highly useful in a variety of cancer studies. In addition, they can bind many proteins and drugs and can be actively targeted to cancer cells by over-expressing cell surface receptors and they are biocompatible in nature with a high atomic number, which directs to greater absorption of kilovoltage X-rays and provides greater contrast than standard agents. Nano-based contrast agents for MRI, OCT, and photoacoustic imaging have lower toxicity, prolonged blood circulation half-life, and the ability to target unique cell surface molecules. The nano agents exhibit better image contrast properties and improved penetration depth than routine contrast agents. They can provide molecular-targeted imaging, analyze biomarkers at the nano-scale, enable intraoperative identification of surgical resection margins, and monitor oral cancer prognosis after treatment. Using nano-based techniques can help clinicians to detect and better monitor diseases during different phases of oral malignancy. This review will focus on such current modalities in the diagnosis & management of oral cancer and pre-cancerous lesions using gold nanoparticles.

Development, Analysis and Evaluation of Arsenic (III) Sensor

Hema. R; Sundararajan M

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 4, Pages 2533-2551

Pollution or contamination plays a major part in dreadful health hazards. Water, the most essential entity for life is being consumed without the knowledge of contaminants. Arsenic (As III) above 10μg/L is regarded to be poisonous to life and causes lung or skin cancer and cardiovascular geotaxis, mutagenic &carcinogenic effects. Contamination of As may not only occurs in under or drinking water but also in sea water which may be a health threat to aquatic lives. Arsenic decontamination need to be strictly adopted upon any form of water purification. A special technique for the determination by express sensing of arsenic ionic level in water was examined on a Smooth or Glassy carbon electrode (GCE) with nano Gold transformation in 0.5 M H2SO4. The nano Gold was setup using a cycling potential continuously ranging from 0.619 to-0.481 V (vs. HAuCl4.3H2O) Chloro Auric Acid in 0.5 M H2SO4 solution. The Gold nano particles are electro- deposited on GCE. The electrode was examined by Differential Pulse Voltammetry (DPV), Anodic Stripping Voltammetry (ASV), Linear Sweep Voltammetry (LSV) and cyclic Voltammetry (CV). After optimization, a LOD (Level Of Detection) of 0.9 parts per billion (ppb) was achieved with a 60s transformation at -0.94 V in 0.5 M H2SO4whereas the WHO’s (World Health Organization) maximum allowable arsenic level is 10 ppb, in drinking water. Mercury Mercurious Sulphate (MMS) was used as standard reference electrode. The developed sensor was evaluated for copper ion interference and was found to have no interfering effect due to ionic copper which is the most potential obstructing ionic species in ionic arsenic determination.

Antiangiogenic Effects Of Ktp Laser Activated Gold Nanoparticles In Prevention Of Neovascularization

Kareem Esam; Lilian Naoum; Maha Fadel; Mahmoud Saber

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 3513-3522

This study examines the antiangiogenic effect of activated naked gold nanoparticles (AuNPS) in dorsal skinfold window chamber rat model after laser coagulation of dorsal blood vessels, to investigate whether the antiangiogenic effect of AuNPs can inhibit reperfusion and neovascularization of photocoagulated blood vessels in an animal model.This study includes 2 groups, each group contains 10 rats, laser group (control group), and AuNPs group. After laser photocoagulation of dorsal blood vessels through the dorsal window chamber fixed on the dorsum of albino rats, the structure of blood vessels and flow dynamics were documented with color digital photography to evaluate photocoagulation and reperfusion. The laser sessions and injection of AuNPs to the epidermal side of the window were twice weekly for 2 weeks. In the laser group (control group), 18 out of 20 photocoagulated blood vessels reperfused within 4-12 days with reperfusion rate 90%. In AuGNPs group 9 out of 22 photocoagulated blood vessels reperfused within 4-12 days with reperfusion rate 40%. Laser activated naked gold nanoparticles have significant antiangiogenic effect, with minimal side effects detected with local use.