Online ISSN: 2515-8260

Keywords : Gram negative bacteria

Comparison of association of ESBL producing bacteria to the duration of the diabetes: An original research

Dr Raveendran SR, Dr Prathish Kumar, Dr Ashok Kumar, Dr Archana Mukunda, Dr Yogesh TL

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 6, Pages 1494-1502

Introduction: Diabetes is a common disease of the current population and its most common complication is recurrent urinary tract infection. Recurrent infections are treated with recurrent antibiotic therapy which leads to emergence of resistant strains of bacteria like ESBL, which can prove to be fatal.
Materials and Methods: Patients with confirmed cases of diabetes were selected based o inclusion and exclusion criteria. The urine samples of all patients were analyzed for bacterial isolates and further analyzed for ESBL producing organisms. ESBL producing strains were tested for their antibiotic sensitivity   
Results: A total of 164 patients comprising of 82 males patients and 82 female patients of both genders in the age groups of 30-60yrs were selected. Out of this 58 females and 32 males had recurrent UTI. 45.73% urine samples showed gram negative isolates and among them 25.33% were found to be positive for ESBL isolates using combination disc method. The predominant micro organism was found to be E coli (33%) in urine samples of both genders. E coli was the largest producer of EBSL (57.9%) followed by K pneumoniae (21.1%). We found a strong association between increasing years of diabetes with ESBL production. 
Conclusion: We found that recurrent UTI in diabetics was most common in sixth decade of life and was noted to be more in female population. E coli and K pneumoniae were the most common GNB isolates as well as ESBL producers respectively. They were resistant to 3rd generation cephalosporin but were highly sensitive to amaikacin and nitrofurantoin.

Microbiological Profile and Antibiotic sensitivity pattern of pyogenic infections in a Tertiary care hospital

Dr. Kiran Kumar Bollepaka , Dr. Sakru Mudavath , Dr. Yarra Sudhakar, Kudurupka Veerender

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 4, Pages 560-567

Bacterial infections that cause production of pus are called pyogenic infections. Pyogenic
infections are associated with high morbidity, so antimicrobial regimens are recommended to
reduce long term complications.
The study was aimed to detect pyogenic bacteria in clinical pus samples and determine their
antibiotic pattern
The study was conducted in Department of Microbiology, Kakatiya Medical College, Warangal
from July 2021 to December 2021. A total of 525 pus samples were collected. Pus samples were
collected with disposable sterile cotton swab and pus aspirates in syringes under aseptic
precautions, and were transported to microbiology laboratory immediately. The pus samples
were inoculated on Blood agar and Mac conkey aga. The plates were incubated at 370 c for 18-24
hours. Identification of organisms were done by using biochemical reactions. Antibiotic
sensitivity test was done by Kirby Bauer disc diffusion method according to CLSI guidelines.
Among 525 samples, 275 (52.38%) samples were positive for growth. Gram negative organisms
were isolated more than gram positive organisms. Pseudomonas aeuriginosa was isolated
predominantly among 275 positive samples, 67(24.3%) out of them was only Pseudomonas
aeruginosa, followed by staphylococcus aureus 19.6%, Klebsiella pneumonia 17%, Escherichia
coli 11.2%. In our study Gram negative organisms were more sensitive to Meropenem,
Piperacillin/Tazobactam and Imipenem; Gram positive bacteria were more sensitive to
Vancomycin and Linezolid.