Keywords : Cholesterol oxidase
BIOCHEMICAL ASSESSMENT OF SERUM PARAOXONASEAND SERUM LIPID PROFILE AS PREDICTOR OF CARDIOVASCULAR DISEASES IN PATIENTS OF PSORIASIS
European Journal of Molecular & Clinical Medicine,
2022, Volume 9, Issue 4, Pages 1222-1232
Background:Psoriasis is the most prevalent chronic inflammatory disease, with an approximate worldwide prevalence of 2-3% . The etiological factor is yet to be known but the genetic factor, trauma, skin infections, drugs, emotional stress like anxiety, alcohol, smoking etc greatly impact the clinical evolution of Psoriasis . Oxidative stress which is induced by the excessive production of reactive oxygen species and inflammatory cytokines that play a significant role in the evolution of severe psoriatic plaques. Serum Paraoxonase is synthesized by the liver and is related with HDL particles.
Materials and Methods: Sample Collection– After 12 hours of fasting, a morning sample of 5 ml of venous blood has been collected from the antecubital vein from each subject under aseptic conditions. Parameters Serum Paraoxonase using paranitrophenol substrate by spectrophotometric method. Serum Lipidprofile Serum totalcholesterol by CHOD-PAP Enzymatic photometric method. Serumtriglycerides by GPO-POD method. Serum Hdl- cholesterol by Direct Enzymatic Cholesterol- DL estimation in the presence of detergent. Serum LDL-Cholesterol estimated by Friedewald formula. Serum VLDL- Cholesterol is estimated automatically by dividing triglyceride by5.
Results: The [mean ± SD] age of the cases was 43.67±11.069 and in the controls 42.8±11.679 years. Statistical analysis showed that there was no statistical difference in age between the two groups (as the P-value is > 0.005). The [mean±SD] basal PON activity in cases was 69.08±13.03 and in controls was 74.46±9.79. There is statistical significance in the basal PON activity between the two groups. The mean serum total cholesterol is increased incases (p value is 0.630) when compared with controls, but it is not statistically significant. there is a high significance in the serum high densitylipo protein (HDL-C) between the two groups: 1. The mean increase in the levels of serum low densitylipo protein (LDL-C) cases are statistically significant when compared with controls. There was a high statistical significance in the serum triglycerides levels between the two groups.
Conclusion: This study was undertaken to study the levels of serum Paraoxonaseandserum lipid profile in 40 patients who are diagnosed with psoriasis to assess the irrole in causing cardiovascular diseases. 2. Inpsoriasis, the elevated levels of triglycerides, low densitylipo protein and very low-density lipoprotein are due to lowered levels of hepatic lipase at acute phase reaction, results in elevation of triglycerides in serum. Decreased levels of HDL-C levels are observed because the systemic Inflammation caused by cytokines. As PON is associated with HDL. The PON levels are affected by the decrease in HDL levels and results in lipidperoxidation along with formation of Oxidised LDL. This OX-LDL accumulates in the smooth muscle of artery and causes atherosclerosis.