Keywords : HPV DNA
COMPARATIVE STUDY OF LIQUID-BASED CYTOLOGY VERSUS HPV DNA FOR SCREENING OF CERVICAL CANCER AT A TERTIARY HOSPITAL
European Journal of Molecular & Clinical Medicine,
2022, Volume 9, Issue 4, Pages 2129-2137
Background: Among Indian women, cervical cancer is the most common form of genital malignancy. Cytology (conventional or liquid-based) & HPV DNA used for diagnostis of cervical cancer. Present study was aimed to compare liquid-based cytology versus HPV DNA for screening of cervical cancer at a tertiary hospital.
Material and Methods: Present study was single-center, prospective, observational study, conducted women between the ages of 21 - 65 years, with white discharge per vaginum, post coital bleeding or irregular bleeding, unhealthy cervix on speculum examination.
Results: In present study, 220 women were evaluated for LBC & HPV DNA testing. Mean age of women was 42.91 ± 6.19 year, mean age at marriage was 17.41 ± 3.63 years & mean duration of sexual life was 26.81 ± 8.63 years. Majority were from lower socioeconomic status (57.27 %). Common chief-complaint was white PV discharge (64.55 %), Post coital bleeding PV (19.55 %) & intermenstrual bleeding PV (15.91 %). On per-speculum examination cervical erosion (40.45 %) was common finding. Common mode of contraception used was tubectomy (49.55 %), while 28.64 % women were not using any contraceptive. In present study, common LBC findings were Inflammatory / reactive changes (48.18 %), Non-specific inflammation (10.91 %), Candida (5.45 %), Trichomonas vaginalis (3.64 %), Bacterial vaginosis (0.91 %), Atrophy (1.36 %). Pathological findings were ASCUS (5.00 %), ASC-H (0.91 %), LSIL (0.45 %), HSIL (0.91 %), HSIL with suspicious of invasion (0.45 %) & Squamous Cell Carcinoma (2.27 %). Normal findings were noted in 18.64 % women while 2 smears were labelled as unsatisfactory smear. HPV DNA was positive in 15.45 % women. HPV DNA was significantly associated with pathological LBC report (low grade, high grade & neoplasia).
Conclusion: HPV testing in comparison to LBC was more effective, but it is costlier for application as population screening tool.