Online ISSN: 2515-8260

Keywords : gram staining

Prevalence of Pseudomonas aeruginosa isolates and it’s antibiogram in the rural tertiary care hospital

Dr. Pranavi V, Dr. Anandteerth R Mathad, Dr. Deepti S Patil, Dr. Rohit Ramesh

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 2, Pages 2667-2672

The emergence of Pseudomonas aeruginosa as a multidrug resistant strain through mutations in the chromosomal genes which regulate the resistance genes has resulted in making the existing antibiotics obsolete and hence is considered as the most challenging bacteria to treat there by leading to worldwide increase in rate of morbidity and mortality. The clinical samples received to the department of microbiology from different ward, ICU’s and OPD’s for culture and sensitivity are subjected to standard microbiological procedures including Gram staining, Oxidase and various other biochemical tests and clinical samples are then streaked on MacConkey agar and Blood agar and are then incubated at 37⁰c for 18-24 hours and are then subjected to antibiotic sensitivity testing. In our study Pseudomonas aeruginosa was highly resistant to Gentamicin-15 (36.5%), followed by Ciprofloxacin-12 (29.2%), Amikacin-10 (24.3%), Piperacillin-9 (21.9%). Pseudomonas aeruginosa was least resistant to Ceftazidime-8 (19.5%), Imipenem-8 (19.5%) which can be considered as sensitive.