Online ISSN: 2515-8260

Keywords : combination disc method

Prevalence of ESBL producing bacteria in recurrent UTI of diabetics: An Original Research

Dr Prathish Kumar, Dr Raveendran SR, Dr Saranya R, Dr Archana Mukunda

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 7, Pages 2458-2467

Introduction: Many Gram negative bacteria are multi resistant as they produce ESBLs which breaks down the ring in the antibiotics there by rendering them ineffective. Infections caused by ESBL can be of great consequences even with common infections like urinary tract infections. Timely detection of drug resistant bacteria is of outmost importance as it plays a role in treatment approach and fatality. 
Materials and methods: 5ml of Clear midline stream urine was collected from 164 patients. The urine samples were careened for bacterial isolates and the presence of ESBL producers were confirmed by combination disc method and tested for antibiotic sensitivity and resistance.
Results: A total of 164 urine samples from 82 males and females were collected. We found that 58 females and 32 males had recurrent UTI. 43 and 38 isolates of ESBL producing bacteria were found by combination disc method in urine samples of females and males respectively. Out of 81 isolates of ESBL producers 43 were from E coli and 20 were from K pneumoniae.    
Conclusion: We found that E coli is the largest producer of ESBL in urine samples of patients with diabetes. ESBL isolates were found more in female urine samples than in males. There was a strong positive association of levels of diabetes with ESBL production. The ESBL were highly sensitive to amaikacin and nitrofurantoin but resistant to 3rd generation cephalosporin. 

Comparison of association of ESBL producing bacteria to the duration of the diabetes: An original research

Dr Raveendran SR, Dr Prathish Kumar, Dr Ashok Kumar, Dr Archana Mukunda, Dr Yogesh TL

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 6, Pages 1494-1502

Introduction: Diabetes is a common disease of the current population and its most common complication is recurrent urinary tract infection. Recurrent infections are treated with recurrent antibiotic therapy which leads to emergence of resistant strains of bacteria like ESBL, which can prove to be fatal.
Materials and Methods: Patients with confirmed cases of diabetes were selected based o inclusion and exclusion criteria. The urine samples of all patients were analyzed for bacterial isolates and further analyzed for ESBL producing organisms. ESBL producing strains were tested for their antibiotic sensitivity   
Results: A total of 164 patients comprising of 82 males patients and 82 female patients of both genders in the age groups of 30-60yrs were selected. Out of this 58 females and 32 males had recurrent UTI. 45.73% urine samples showed gram negative isolates and among them 25.33% were found to be positive for ESBL isolates using combination disc method. The predominant micro organism was found to be E coli (33%) in urine samples of both genders. E coli was the largest producer of EBSL (57.9%) followed by K pneumoniae (21.1%). We found a strong association between increasing years of diabetes with ESBL production. 
Conclusion: We found that recurrent UTI in diabetics was most common in sixth decade of life and was noted to be more in female population. E coli and K pneumoniae were the most common GNB isolates as well as ESBL producers respectively. They were resistant to 3rd generation cephalosporin but were highly sensitive to amaikacin and nitrofurantoin.