Online ISSN: 2515-8260

Keywords : extract


RESEARCH TECHNOLOGY OF PRODUCTION OF HERBAL AND NATURAL PRESERVES

Mashhura Zokirova; Dilorom Saribaeva; Sohiba Xojieva

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 325-333

This research work is devoted to the technology of production of dietary and herbal natural preservatives for people with diabetes. Scientific work on hydrolysis of inulin from Jerusalem artichoke with citric acid, acid-rich fruit juices was carried out. The hydrolyzate was purified, transformed into syrup, and used as a sugar substitute in the preparation of canned sugar.

DETERMINATION OF THE NUMBER OF BIOLOGICALLY ACTIVE SUBSTANCES PREPARING THE MEDICINE PROPERTIES OF TREES FOR CORRECTION OF ANIMAL POISONED PESTICIDES

Parida Mirkhamidova; Dilnoza Babakhanova; Mavjuda Rahmatullayeva; Rano Alimova; Gafurjan Mukhamedov

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 886-898

The total amount of biologically active compounds - antioxidants, flavonoids, and vitamin C in different trees for example in Morusnigra L., Prunuspersica L., Serasus Vulgaris L., and Juglansregia L. leaves were found at different seasons. The results showed that the highest concentration of antioxidants in spring was found in walnuts and peaches than in the summer season. The number of walnut leaves was about 9.105 ± 0.05 mg/g, and in peach, leaves were about 9.082 ± 0.075 mg/g. In spring, the content of flavonoids in the leaves of grapes was high and amounted to 4721 ± 0.521 mg/g, in the summer their number was 3825 ± 0.255 mg/g, and in the fall there was a decrease in flavanoids to 1.460 ± 0.2253 mg/g. In the spring, in the stalks of grapes, flavonoids were 4.386 ± 0.046 mg/g, in cocoons 0.348 ± 0.023 mg/g.

ISOLATION OF Α-AMYLASE INHIBITORS FROM METHANOL FRACTION OF THE ENDOPHYTIC FUNGUS PENICILLIUM BREVICAULE ALBA THOM

Saodat Nasmetova; Tashkhan Gulyamova; Dilorom Ruzieva; Iqbol Mukhammedov; Lilia Abdulmyanova

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2174-2181

Abstract. Postprandial hyperglycemia is a significant risk factor involved in type 2
diabetes. The elevated level of postprandial hyperglycemia is caused by carbohydratehydrolyzing
enzymes, α-amylase, and α-glucosidase. Inhibition of these enzymes appears to
be an effective means for diabetes management. Recent studies suggested that endophytic
fungi of some medicinal plants produce α-glucosidase and α-amylase inhibitors. The
endophytic fungus P. brevicaule alba-CC 200, which exposes inhibition of α-amylase, was
isolated from the Celosia cristata plant. This article presents data on the purification of
inhibitory compounds from the methanolic extract of Penicillium brevicaule alba Thom-
CC200. Fraction of G-7 with the highest inhibitory activity of 76.5% isolated by adsorption
column chromatography. The yield of dry samples was more than 18 weight % (w/w) with
more than 60% purity. The HPLC and LC-MS of fraction G-7 indicated that the inhibitory
activity is these fractions associated with the triterpene saponins.

Quantitative And Qualitative Analysis Of A Liquid Extract Obtained On The Basis Of Wormwood

Yuldasheva Shahlo Khabibullaevna; Tukhtaev Khakim Rakhmonovich

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 3336-3345

The work is devoted to the development of the technology of composition and the study of the chemical composition of the liquid extract based on bitter wormwood - Artemisia Absinitum L. grown in the territory of Uzbekistan. The addition of tansy and pumpkin seeds to the composition of wormwood makes it possible to obtain liquid dosage forms with an enhanced anthelmintic effect. The study used methods of spectrophotometry, thin layer chromatography, qualitative chemical analysis, ionization plasma analysis, etc. The liquid extract was standardized by quantitative determination of flavonoids. A number of factors have been identified that influence the optimal technology for obtaining a liquid extract from plant materials. A method has been developed for the quantitative determination of the amount of flavonoids using spectrophotometry in the wavelength range of 350-450 nm. The content of the sum of flavonoids in extracts varies in terms of a standard sample of rutin in the range from 1.2860 to 1.5871% (mg / ml). For elemental analysis, the method of inductively coupled plasma mass spectrometry was used, on the basis of which important macro- and microelements were found in the composition of the liquid extract.
The studied indicators of the microbiological purity of the extract comply with the requirements of the State Pharmacopoeia XI issue 2.