Online ISSN: 2515-8260

Keywords : endophytic fungi


Phytochemical analysis of α-amylase- inhibiting secondary metabolites of endophyte Penicillium brevicaule alba Thom

Gulyamova T.G.; Nasmetova S.M.; Ruzieva D.M.; Mukhammedov I.I,; Kadyrova G.Kh.; Karimova F. A.

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 829-836

The present work was aimed to identify phytochemicals in methanol extract P. brevicaule alba Thom - CC200, obtained from C. cristata , by qualitative and TLC analysis and evaluation of α-amylase inhibitory activity in vitro. It was determined the presence in the extract of glycosides, terpenoids, and saponins. The partitioning of the extract by TLC showed that inhibition of enzyme activity at 50,3% and 37,6% is associated with two compounds of saponin nature.

ISOLATION OF Α-AMYLASE INHIBITORS FROM METHANOL FRACTION OF THE ENDOPHYTIC FUNGUS PENICILLIUM BREVICAULE ALBA THOM

Saodat Nasmetova; Tashkhan Gulyamova; Dilorom Ruzieva; Iqbol Mukhammedov; Lilia Abdulmyanova

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2174-2181

Abstract. Postprandial hyperglycemia is a significant risk factor involved in type 2
diabetes. The elevated level of postprandial hyperglycemia is caused by carbohydratehydrolyzing
enzymes, α-amylase, and α-glucosidase. Inhibition of these enzymes appears to
be an effective means for diabetes management. Recent studies suggested that endophytic
fungi of some medicinal plants produce α-glucosidase and α-amylase inhibitors. The
endophytic fungus P. brevicaule alba-CC 200, which exposes inhibition of α-amylase, was
isolated from the Celosia cristata plant. This article presents data on the purification of
inhibitory compounds from the methanolic extract of Penicillium brevicaule alba Thom-
CC200. Fraction of G-7 with the highest inhibitory activity of 76.5% isolated by adsorption
column chromatography. The yield of dry samples was more than 18 weight % (w/w) with
more than 60% purity. The HPLC and LC-MS of fraction G-7 indicated that the inhibitory
activity is these fractions associated with the triterpene saponins.