European Journal of Molecular & Clinical Medicine

Red dragon fruit (Hylocereuspolyrhizus) it is an essential source of betacyanin which is responsible for its red purple pigment. The purpose of this study was to determine the betacyanin content of alcoholic H. polyrhizus (red dragon fruit) flesh extract as an alternative counter stain for semen smear. This study utilized an experimental comparative design method. Samples were subjected with 20ml of 100% methanol in every 100 ml of H. polyrhizus flesh extract and to a series of temperatures: 30oC, 60oC, and 100oC for 5 minutes in the water bath to determine its color stability. This is followed by storing it at low temperature (4oC) without light exposure for 48 hours. The results of this study showed that the pigments were stable at 100oC heat treatment for 5 minutes in water bath and it stained excellently with a rate of 2.48 this signified that the standard eosin Y-nigrosin and alcoholic H. polyrhizus flesh extract have the same color affinity in staining sperm cells. Results also showed that the betacyanin obtained from the H. polyrhizus flesh extract heated at 100oC was effective as an alternative counterstain for semen smear.

The present work was aimed to identify phytochemicals in methanol extract P. brevicaule alba Thom - CC200, obtained from C. cristata , by qualitative and TLC analysis and evaluation of α-amylase inhibitory activity in vitro. It was determined the presence in the extract of glycosides, terpenoids, and saponins. The partitioning of the extract by TLC showed that inhibition of enzyme activity at 50,3% and 37,6% is associated with two compounds of saponin nature.

This article highlights the study on the determination of differential heat, isotherm, thermokinetics and entropy of water, carbon dioxide, normal heptane, benzene, methanol, ethanol adsorption on ZSM-5 zeolites, using adsorption-calorimetric method at a temperature of 303 K. The type and number of formed ion-molecular complexes, as well, the mechanism of adsorption of the studied molecules were ascertained. In the zeolite structure, thermokinetics of adsorption and the discovery of the migration laws of exchange cations are considered the adsorption energy of gases and vapors in a flawless zeolite. From the beginning to the saturation, the adsorption isotherm was described by the two- and three-term mathematical equations of STMP.

A series of synthesis of some substituted isoxazole derivatives using efficient ultra sound sonication method in methanol solvent. Firstly the synthesis of different substituted chalcones using substituted aldehydes and substituted acetophenones using triethylamine as base in ethanol solvent under sonication. In second step synthesized substituted chalcones is reacted with two equivalents of hydrochloride salt of ammonium hydroxide, sodium oxalate and methanol under sonication at 30-35OC to afford a substituted isoxazole derivatives in 90-98% of yields. The structures of the synthesized compounds were confirmed on the basis of NMR, and MS analysis. This method has a several advantages over current reaction methodologies, such as shorter reaction times, simple work-up procedure and good percent yields.