Online ISSN: 2515-8260

Keywords : genotype

Analysis of the role 1G / 2G polymorphism Analysis of the role 1G / 2G polymorphism in the MMP1 gene development and clinical course of cervical intraepithelial neoplasia

I.A. Kamilova; J.E. Pakhomova; D.K. Nadjmutdinova

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 850-859

In the present work, the frequency of occurrence of polymorphic variants 1607 1G / 2G (rs 1799750) of the MMP1 gene encoding enzymes of collagen metabolism and its genotypes in patients with cervical intraepithelial neoplasia and healthy women in Tashkent was first investigated. A statistically significant association of 2G allele carriage with a risk of developing CIN (χ² = 15.4; P≤0.001) and unreliable with carriage of the heterozygous genotype 1G / 2G (χ² = 1.36; P≥0.243) were established. In the control group of patients, a statistically significant predominance of the 1G allele (χ² = 29.4; P≤0.001) and the homozygous genotype 1G / 1G (χ² = 19.6; P≤0.001) was found. The highest values of the carriage of the 2G allele and homozygous genotype 2G / 2G were found at CIN and at CIN ΙΙΙ. Thus, the study of polymorphism rs 1799750 of the MMP1 gene is promising for predicting the course of cervical intraepithelial neoplasia and can be used to form risk groups for the development of this pathology.
The frequency distribution of alleles and genotypes of the polymorphic marker rs1799750 of the MMP1 gene in patients with CIN did not correspond to the canonical Hardy-Weinberg distribution due to an increase in the frequencies of homozygous genotypes and a decrease in the frequency of the heterozygous genotype; in the control group, the frequency distribution of alleles and genotypes of the polymorphic marker rs 1799750 of the MMP1 gene corresponded to the canonical Hardy-Weinberg distribution.

Genotype Variants Of Dengue Virus On Dengue Hemorrhagic Fever (Dhf) Suspect: Cross Sectional Study In Health Facilities In Semarang City, Indonesia

Martini Martini; Syahilla Efriana; Rini Fajarini; Ari Udiyono; Retno Hestiningsih; Atik Mawarni; Indranila Kustarini

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 8, Pages 730-740

Semarang has been reported as an endemic area of dengue hemorrhagic fever (DHF), yet continuous data of molecular epidemiology related to dengue fever is not available. The aim of this study was to analyze the characteristics of virus strains, host suspected dengue, and the dynamics of dengue fever in Semarang City. The design of this study was observational description with cross-sectional design. Seventy three DHF suspected patients from two hospitals and seven health centers in Semarang City were sampled during 2017- 2018. Reverse Transcription Polymerase Chain Reaction (RT-PCR) method was used to examine serum suspected dengue and sequence serotypes and genotypes of dengue virus. The results showed that the highest frequency to be exposed by DHF was infant and children; while, the number of male and female with suspected dengue was relatively the same. The dominant serotype was DENV-3, DENV-1, and mix of DENV-2 and DENV-3; while, the circulation of DENV-1 genotype I and DENV-3 genotype I were identified as Indonesian local endemic strain causing epidemics in Indonesia.

Performance Of Fiber Output And Fiber Length In Inter Variety Hybrid Families Of Middle Fiber Cotton

Madartov Bakhrom Quvondiqovich; Kholmurodova Guzal Ruzievna; Ergashev Jakhongir Abduganievich; Juraev Sirojiddin Turdikulovich; Baratova Anisa Razzakovna; Shomonova Gulnoza Erkinovna

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 2383-2389

Purposefulness of using the performed families О-445, О-580, О-455 in the genetic and breeding researches for improving of fiber output and the families of О-580, О-455, О-520 to improve the fiber length on the dependence of their genotypes has been presented in this paper. In general, similarity of a high dominance of varieties in the positive indexes on the fiber output and fiber length in combination of various soil-climatic zones was noted.