Online ISSN: 2515-8260

Keywords : Enzyme


EVALUATION OF A BREEDING MATERIAL FOR RESISTANCE TO VERTICILLIUM DAHLIAE USING MARKER PHYTIMMUNITY ENZYMES

DSc. Kurbonov A.Y .; prof.Avtonomov V.A .; prof. Axunov A.A .; DSc.HashimovaN .

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 1044-1051

In the world's cotton-growing countries, cotton is cultivated on the territory of 89 countries, on a total area of more than 30 million hectares, from which more than 22.4 million tons of cotton fiber are obtained. Today, there are problems in the production of high-quality cotton fiber yield. One of these problems is pathogens that cause significant damage to cotton production, with losses in the world amounting to 12-15%. In the world, considerable attention is paid to the study and control of the pathogen Verticillium dahliae Klebahn, which affects cotton.
The problem of breeding wilt-resistant varieties of cotton is complicated by the search for new methods and donors of resistance to the pathogen. It is necessary to improve the method of selection of parent pairs during hybridization and qualitative assessment of interspecific hybrids at the early stages of the breeding process in order to increase the efficiency of breeding, speed up the process of introducing new varieties of cotton into production.The selection must be carried out on the basis of physiology and biochemistry of signs of resistance of the initial breeding material. The initial stage of selection should be based on test signs of resistance, which are associated with the catalytic activity of some enzymes involved in the formation of phytoimmunity against fungal infections (peroxidase, phenylalanine ammonialyase, polyphenol oxidase).The greatest interest of researchers is attracted by protective mechanisms, including the processes of lignification of cell walls and the biosynthesis of phenolic phytoalexins. These mechanisms simultaneously create a mechanical and chemical barrier to the penetration of fungal structures into the cell, preventing the spread of the pathogen.

The Role Of Biologically Active Substances In The Blood In Increasing The Productivity Of Sheep

Shavkat Mukhitdinov; Dilmurod Aliyev; Komil Ismoilov; Gulnora Mamurova

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 2704-2710

Physiological, immunogenetic, selection, technological, biotechnological, and other methods to increase the productivity of karakul sheep are used to increase their productivity, as well as biologically active substances that increase the productivity of karakul sheep.

Influence Of Karate On The Activity Of Enzymes Of The Anti-Oxidizing System Of Rat Liver Protection And Ways Of Their Correction

Parida Mirkhamidova; Dilfuza Tuychieva; Dilnoza Bobokhonova; Mashkhura Parpieva

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 3, Pages 3757-3765

As a result of research, it has been established that synthetic karate pyrethroid causes functional changes in antioxidant enzymes in microsomes and mitochondria. An important aspect of our study was the comparison of the taxation rate when animals were poisoned with a karate pesticide and when animals were protected from poisoning with an antioxidant of plant origin. The results obtained indicate that the plant antioxidant factor (PAF) has protective properties and accelerates the restoration of enzyme activity.

Palm Kernel Cake Reaction of Fermentation Results of Mannanase Activity Bacillus Subtilis ATCC 6633

Cashin Japlin; Achmad Toto Poernomo; Isnaeni .

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 3940-3946

Mannanase is an enzyme which is capable of breaking down mannan substrate by hydrolyzing the β-1.4-manosidic linkage between mannose and mannose. Mannanase can be applied to feed, paper, pharmaceutical, food, detergent, oil and gas industries. In the pharmaceutical industry, mannanase can be used for controlling drug release from composed matrix from cross-linked galactomannansThe objective of this study is to discover the activity of the bacterium Bacillus subtilis ATCC 6633 in producing mannanase through a fermentation process using PKC mediumThis kind of research is true experimental research with replications.The research results recommend to increase the utilization of palm kernel cake (PKC) as a medium and Bacillus subtilis ATCC 6633 as a source of mannanase, after production optimization, either in laboratory, pilot or commercial scale, by changing various factors which affect the productivity of Bacillus subtilis ATCC 6633 to determine the optimal time of fermentation, so that the result produces maximum mannanase activity.Bacillus subtilis ATCC 6633 is capable of producing mannanase through the fermentation process using PKC as a medium. Furthermore, the mannanase Bacillus subtilis fermented result activity ATCC 6633 was using 0.25% of PKC as a medium produced a mannanolytic index of 4.0 ± 0.98.