Online ISSN: 2515-8260

Keywords : Platelets


Nilam Bhasker, Bharti Parashar, Meeta Agnihotri

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 4, Pages 3349-3358

Introduction: Nowadays, platelet counting is employed as a routine method owing to the emergence of dengue fever from the past few decades. Manual methods yield varying outcomes while sometimes automated methods produce inappropriate outcomes. Hence, the goal of this study is to evaluate the diagnostic accuracy of manual modality of platelet count with respect to automated analyzers. Additionally, this study was also analyzed the correlation between manual and automated analyzer.
Materials and Methods: The current study was carried out in the Department of Pathology, Employee’s State Insurance corporation (ESIC) Hospital, Sarojini Nagar, Lucknow (U.P.) on a group of 600 patients (375 female and 225 male) including both indoor and outdoor settings  from 1st November to 30th November, 2021 in a one month duration. The blood samples were collected in tubes containing K3-EDTA (tri-potassium ethylenediamine-tetra-acetic acid). Manual method of platelet counting was performed by slide method with Leishman stain while automated counting was carried out by using Mindray BC-6200, 5 part auto-hematology analyzer.
Results: A total of 600 patients have studied with the mean platelet count in automated analyzer was 1.16±0.99 ×103 /µL and by manual method with Leishman stain was 1.23±1.03 lacs /mm3. A positive correlation was observed between automated analyzer and manual platelet count (r=0.837, p=0.00).     
Conclusion: When the platelet counts are very low, manual method of platelet counting should be done carefully to exclude clumping or irregular distribution of platelets. 

Relationship Between Platelet, Hematocrit and Leukocyte with Dengue Severity in Bengkulu City, Indonesia

Dessy Triana; Annelin Kurniati; Gayatri Ghea Wirastari

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 10, Pages 2305-2311

Establishing the correct diagnosis of the clinical stage and the severity of dengue patients is very important to determine the prognosis. Examination of platelets, hematocrit and leukocytes for each clinical stage of dengue can help group and manage patients based on their clinical degree. This study aimed to determine the relationship between platelet, hematocrit and leukocyte examination results with the clinical degree of dengue based on WHO. This study used a cross-sectional study design with a sampling technique using consecutive sampling from July-November 2020 in 5 hospitals at Bengkulu City with total sample of 35 patients. The results found a strong relationship between hematocrit levels and the degree of severity of dengue (p = 0.001; r = 0.637). However, there was no significant relationship between the number of platelets (p = 0.826; r = -0.039) and leukocytes (p = 0.554; r = -0.103) with the severity of dengue. Laboratory tests are crucial to establish the clinical degree of dengue and disease’s prognosis.


I.I. Bakhriev .; SH.Yu., Beknazarov; S.J. Khojanazarova; E.A. Eshbayev

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2473-2482

Abstract: It is known that, against the background of the progression of chronic hepatitis,
significant changes in blood elements are occurring. The number of red blood cells, the
level of hemoglobin, the color index, the content of reticulocytes, platelets and leukocytes
in patients with chronic diffuse diseases of the liver are significantly lower than in people
without diseases of the hepatobiliary system, and blood counts decrease as the activity of
inflammation in the liver and the transformation of chronic hepatitis B increase cirrhosis
of the liver. The purpose of this work is to identify the features of the hemogram in patients
with cirrhosis of the liver.