Online ISSN: 2515-8260

Keywords : Healing

The Role of Platelet-Rich Plasma in Inducing Musculoskeletal Tissue Healing in Chronic Tendinopathies

Dr. Uma Durga Vinod D, Dr Rakesh Chandra M, Dr Jakku Kranthi, Dr Pradeep Chandra Chetamoni

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 1618-1623

Background: Platelet-rich plasma [PRP] has received increasing interest across many
musculoskeletal disciplines and has been widely applied clinically to stimulate tissue
healing in numerous anatomical regions. The known actions of platelet-derived factors
suggest that PRP may have significant potential in the treatment of pathological
conditions of cartilage, tendon, ligament, and muscle.
Purpose: The aim of this manuscript is to review current literature regarding the
biology of PRP and the efficacy of using PRP to augment healing of tendon ligament
and muscle injuries, as well as early osteoarthritis.
Methods: A comprehensive literature review of musculoskeletal applications of PRP
was performed, including basic science and clinical studies such as randomized
controlled trials, case controlled series, and case series.
Results: The most compelling evidence to support the efficacy of PRP is for its
application to tendon damage associated with lateral and medial epicondylitis. Although
some promising studies have been reported supporting the use of PRP in osteoarthritis
and ligament and muscle injuries, it currently remains unknown whether PRP
effectively alters the progression of osteoarthritis or aids the healing of ligament and
muscle tissues.
Conclusion: The rationale for the use of PRP to improve tissue healing is strong, but the
efficacy for many musculoskeletal applications remains unproven. PRP has been shown
to be a safe treatment. A number of questions regarding PRP remain unanswered,
including the optimal concentration of platelets, what cell types should be present, the
ideal frequency of application, or the optimal rehabilitation regimen for tissue repair
and return to full function

Role of Parenteral Amino Acid in Patients of Diabetic Foot Ulcer

Dr. Ankit Mohan Das,Dr. Jagadamba Sharan, Dr. Mohit Biswas

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 2071-2079

Aim: To analyse the role of amino acids among subjects with type 2 diabetes having foot
Material and method: The present study was conducted in the Department of Surgery,
Rohilkhand Medical College and Hospital, Bareilly from February 2020 to March 2021. The
study comprised of 60 patients, aged 18 years and above, suffering from diabetic foot ulcer.
Patients were divided into two groups i.e. group A and B. Patients of group A continued to
take diabetic diet as they were taking before while patients of group B were given IV amino
acid (200ml) on alternate day till 15th day in addition to the diet they were taking before.
Ulcers of all patients were properly cleaned and dressed under aseptic condition with normal
saline and povidone iodine everyday, wound debridement was done as required. Ulcers were
assessed on day 0, 5, 10 and 15.
Results: Mean cholesterol increased in group A while it decreased in group B at different
intervals with statistically insignificant difference as p>0.05. At the end of study, normal
healing was found among 26.67% and 66.67% of the subjects in group A and B respectively.
Major haematoma was reported only in group A and that among 10% of subjects. Hence
healing was observed better among group B subjects.
Conclusion: It can be concluded from the results that among subjects with diabetic foot
ulcers, amino acid administration leads to better healing and satisfaction among the patients.
Therefore, amino acids supplement should be given to diabetic foot ulcer subjects

Comparative study of staplersvs simple interrupted vs sub-cuticular method of skin closure of surgical wounds

Dr. Ankit Mohan Das, Dr. JagadambaSharan, Dr. MohitBiswas

European Journal of Molecular & Clinical Medicine, 2022, Volume 9, Issue 3, Pages 1926-1933

Aim: To compare staplers vs simple interrupted vs sub-cuticular method of skin closure of
surgical wounds.
Material and method:The present randomized prospective study was conducted in the
Department of Surgery at Rohilkhand Medical College and Hospital, Bareilly from January
2020 to October 2021 among 150 subjects who underwent surgical procedures. Equal number
of patients was allotted to 3 groups by random envelope allocation method i.e. Group
A(Stapled skin closure), Group B(Interrupted skin closure) and Group C(Sub cuticular skin
closure). Following the completion of closure, an antiseptic medicated cream was applied
followed with a protective dressing for the first 24–72 h. Subcutaneous Drains were placed in
selective cases according to infection and were kept till the drain content was minimal. All
patients were given IV antibiotics for 5–7 days postoperatively. The closures were removed
after an interval of 10–14 days, first removing the alternate sutures and then the remaining
sutures after few days and pain on removal was recorded using VAS.
Results: Postoperative pain score at discharge was least in sub-cuticular group followed by
stapled and interrupted skin closure group, though statistically there was no difference.
Conclusion: In this we found that time required for skin closure (in seconds) was least in
stapled skin closure group while pain and POSAS score was minimum in sub-cuticular skin
closure group. Wound infection was present maximum and minimum among sub-cuticular
and interrupted skin closure group respectively


Baykulov Azim; Abdullayev Davlat; Narzikulov Rustam; Oripov Rustam; Yermanov Rustam; Mamirov Vasliddin; Islamov Nurali

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 3, Pages 1785-1808

Background: Despite the vast experience of treating burns in surgery, a large number of victims become disabled; therefore they represent a serious medical and social problem.
Methods: The study included 120 white outbred male rats, which were simulated an experimental 3-degree topical burn and were divided into 4 groups according to the type of treatment substance. The burn was simulated by immersing the depilated skin area in boiling water. Studies of wound healing were carried out in three series, respectively, on the third, seventh and tenth days.
Results: We found that the local application of chitosan derivatives at an early stage leads to healing of the wound defect in rats in our experimental model. This was associated with a decrease in endogenous intoxication, lipoperoxidation, and internucleosomal degradation compared to the control group.
Conclusion: Thermal damage is accompanied by increased internucleosomal skin degradation. Compared with levomecol, chitosan derivatives more effectively reduce the degree of internucleosomal degradation, especially on the 10th day of the experiment.

Non-Surgical Endodontic Approach for Management of Periapical Lesions with 6 months follow up: A Case Series.

B. Sravan Kumar; M.S. Rangareddy; Basa Srinivas Karteek; Chavva Lakshmi Charan Reddy; Md Abdul Wahed

European Journal of Molecular & Clinical Medicine, 2021, Volume 8, Issue 2, Pages 1524-1531

The success of root canal treatment depends on thorough disinfection of the root canal system, and to provide fluid impervious seal which in turn halt the progress of the periapical infection. The oral microorganisms from the degenerated pulp tissue are responsible directly or indirectly for most of the periapical lesions. For the treatment of these lesions there are surgical and non-surgical methods. Surgical removal of the periapical lesion without proper root canal disinfection and obturation will result in improper healing of lesions whereas in non-surgical root canal therapy the lesions heal if proper cleaning, shaping and obturation of root canal are done. This clinical case series highlights the follow-up results of three cases demonstrating the resolution of periapical lesion through nonsurgical approach and confirms that periapical lesions respond favorably to non-surgical treatment

Assessment Of Outcome Profile Of Patients With Peri-Apical Surgery- An Observational Study

Dr. Tejveer Singh; Dr. Naresh Kumar; Dr. Rupinder Bansal; Dr. Gursandeep; Dr. Mehak Goyal; Dr. Himanshu Sood

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 8, Pages 3673-3677

Background: The present study was conducted to assess profile of patients undergoing apical surgery.Materials & Methods: 56 patients were selected for peri- apical surgery of both genders. Follow-up was done at 1, 3, 6, and 12 months and every 6 months thereafter. Clinical and radiographic examinations were performed at each recall.
Results: Teeth involved was maxillary incisor in 12, maxillary cuspids in 20, mandibular incisors in 10 and mandibular cuspids in 14 cases. Prostheses were present in 25 and absent in 31 cases. Bony destruction pattern was apical in 45 and apico-marginal in 13 cases. Post was present in 14 and absent in 42 cases. The maximum healing was seen in maxillary incisor in 67% followed by maxillary cuspids in 52%, mandibular incisors in 65% and mandibular cuspids in 54%, 56% in teeth with prostheses, 82% in apical bone destruction and 76% in teeth with post. The difference was significant (P< 0.05).
Conclusion: There was better healing in maxillary incisors, teeth with prostheses present, teeth with apical bone destruction and teeth with post.

Comparative evaluation of healing after periodontal flap surgery using isoamyl 2- cyanoacrylate (bioadhesive material) and silk sutures: A Study Protocol

Dr. Diksha Agrawal; Dr. Priyanka Jaiswal; Dr. Deepika Masurkar

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 2044-2051

Abstract: Background- The main goal in periodontal surgery is the healing by primary
intention because there is less scarring, more rapid healing and reduced discomfort.
Cyanoacrylate is a tissue adhesive which provides benefits to the patient as a faster healing
and less postoperative pain. Flap surgery for periodontal reattachment, demands close
postoperative adaptation for the gingival tissue onto the prepared tooth surface. Objective- To
evaluate the healing after periodontal flap surgery using isoamyl 2‑ cyanoacrylate and silk
sutures. Material and Methods- In this study, 40 patients of systemically healthy will be
selected. Groups will be divided into test and control which consist of 20 defects in each.
Informed written consent will be obtained from all the subjects before the surgery. Clinical
measurements like Plaque index, Papillary bleeding index, Probing pocket depth, Healing
index will be recorded. The test group will be treated by isoamyl 2‑ cyanoacrylate, while the
control group will be treated by Silk suture after periodontal flap surgery. Patients will be
recalled at 1, 3, 6 months post surgically for periodontal re-evaluation. Expected Result- When
all the parameters will be compared at 6 months post-operatively to baseline data, both the
treatment group (test and control) will show significant CAL gain, PPD reduction. Test group
will be found better healing as compared to control. Conclusion- Both the treatment group
(test and control) will show significant CAL gain, PPD reduction. This study will be achieved
effective healing response after periodontal flap surgery with sutures as compared to


Prabhav Kumar Iyer; Dr. Deepika Rajendaran; Dr. Sarvana Dinesh S.P

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 660-676

Hyperglycemia is a condition in which there is an increase in the blood glucose level of the person. It is one of the most common systemic conditions in middle aged Indians. It is important for a dentist and dental students to be aware of the condition especially while performing invasive procedures as delayed wound healing, coma and hypoglycemic shock are some of the most common complications encountered in some patients. Hence, it is important to know about this condition to prevent any kind of medical emergency on a dental chair. The aim of the study was to assess the knowledge and attitude of dental students while managing hyperglycemic patients indicated for extraction. This study was carried out in a self administered questionnaire format among the students of a dental university. The questionnaire was created on an online platform called “Survey Planet” and was filled by 100 students. The questionnaire consisted of the major aspects of knowledge on hyperglycemia and how students will manage the patient before performing the extraction procedure on them. Based on the tabulated results, it was seen that dental students have greater knowledge towards dealing with hyperglycemic patients indicated for extraction. 64% said that they can perform extraction on a hyperglycemic patient. 93% said that they would test an elderly patient to check their blood glucose level before the procedure. 72% were aware of the management of hyperglycemia. 45% of the participants said that they would do the treatment only after the patient consulted a physician. 11% said that they would deny the treatment. 82% of the participants said that they would keep the appointment for the extraction in the morning. Thus we can conclude that dental students have good knowledge and attitude toward managing hyperglycemic patients indicated for extraction