Online ISSN: 2515-8260

Keywords : Oral submucous fibrosis

Comparative evaluation of Immunohistochemical expression of MT-1 MMP, TIMP-1, TGF-β1, α-SMA in oral submucous fibrosis and oral submucous fibrosis with coexisting Oral Squamous cell carcinoma

Sharma Nidhi; Gawande Madhuri

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 2, Pages 1994-2002

Abstract: Background : „Oral submucous fibrosis” (OSF) is chronic, progressive, and
irreversible disease but also as oral potentially malignant disorder with high probability of
malignant transformation. From a clinical as well as the histological point of view it
appears to be of major importance to establish and understand the molecular nature of the
mechanism of malignant transformation in OSF .So that these molecules could be targeted
to formulate a standard treatment for high risk cases of OSF patients in need for better
prognosis. Material and Methods: 25 Cases of “oral submucous fibrosis .” and 25 Cases of
oral Submucous Fibrosis with coexisting oral squamous cell carcinoma will be analyzed.4
μm thick sections of formalin fixed paraffin embedded blocks were immunohistochemical
stained with Polyclonal rabbit MT-1 MMP antibody , Monoclonal mouse TIMP-1 antibody
,Monoclonal mouse TGF-β1antibody , Monoclonal mouse α-SMA antibody in each case
Statistical Analysis: Sample size formula based on difference between two proportions:
This study uses the following formula for the sample size n: n = (Zα/2+Zβ)2 * (p1(1-
p1)+p2(1-p2)) / (p1-p2)2,
Discussion: The Incidence of malignant potential in OSF has been estimated to be 7-13%².
After years of persistent efforts and research, satisfactory and definitive treatment modality
still eludes us. These markers could be used on regular basis to assess malignant potential
of OSMF. This will help to formulate and decide the standard treatment plan with a better
regime of target drug therapy


Dr Swetha Bhat; Dr Senthilnathan Periasamy; Dr Murugaiyan Arun

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 927-935
The purpose of this study was to highlight various pharmacotherapeutic treatment modalities and to
compile them to assist in early intervention and management of Oral Submucous Fibrosis
This study was conducted on 92 patients who reported to the Department of Oral Surgery and Oral
Medicine, Saveetha Dental College, Chennai. They were clinically diagnosed with oral submucous with
standard data grading and were given a combination of treatments currently available. Out of 92 cases,
85(92.4%) were males and 7(7.6%) were females which showed a male predominance with a ratio of 12:1
in the prevalence of Oral Submucous Fibrosis. The age group affected the most was 35-50 years (54.28%).
Oral systemic antioxidants was prescribed to 54% of the patients and was the most frequently used
treatment modality in the pharmacotherapeutic management of Oral Submucous Fibrosis. Management of
Oral Submucous Fibrosis has been the subject of controversy ever since it was first stated in 1952. This
study attempts to compile the available treatment and its current status to assist early intervention of the


Nivethitha R; Dr.Deepika Rajendran; Dr. Saravana Dinesh S.P

European Journal of Molecular & Clinical Medicine, 2020, Volume 7, Issue 1, Pages 1221-1236

Oral submucous fibrosis(OSMF) is a potentially malignant disorder of oral mucosa affecting mainly the population in south and southeast Asia. OSMF is precancerous condition caused by areca nut chewing characterised by restricted mouth opening, burning sensation and stiffness and blanching of oral mucosa.Complete regression of the condition had not been achieved in all cases with any of the present treatment regimen.The aim of the study was to assess the knowledge among dental students about the significance of Oral Submucous Fibrosis and it’s management. The questionnaire based study was conducted among 100 participants. A self administered questionnaire was used. The questionnaire based study was conducted through an online forum , google form software. The questions were formed to observe knowledge,attitude,and practical approach of dental students towards treating OSMF patients attending private dental hospitals .The data collected were stored and results were analysed by SPSS software. Out of 100 participants, 55% participants reported that females have an increased prevalence of OSMF compared to male. Majority of participants were aware that betel quid were the most common habits and blanching mucosa were the common features associated with OSMF. 60 % of participants were aware that stopping gutka / pan chewing habits alone will not resolve the problem in patients having trismus. Within the limits of the present study, students showed good knowledge on the various clinical and diagnosis aspects of Oral submucous fibrosis, however the knowledge on the management aspect of oral submucous fibrosis was moderate. A better knowledge of OSMF will endure safer health care services for the population.